Simultaneous Determination of Tocotrienols, Tocopherols, Retinol, and Major Carotenoids in Human Plasma

Lee, Bee‐Lan; New, Ai-Li; Ong, Choon Nam

doi:10.1373/clinchem.2003.022681

Cited by 98 publications

(96 citation statements)

References 36 publications

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“…So the conclusion is that the sample of rice bran treated with HCL gives the best results regarding unsaturated fatty acids. The above results reveal the findings of Lee, Randall and Lakkakula et al, [22][23][24] (Figure 5). …”

Section: Free Fatty Acid (Ffa) Status Of Rice Bransupporting

confidence: 88%

Inactivation of Lipase Enzyme by Using Chemicals to Maximize Rice Bran Shelf Life and its Edible Oil Recovery

Akhter¹,

Afzal²,

Haider³

et al. 2015

J Nutr Food Sci

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Rice bran is the by-product of the rice milling industries so in view of growing need and consciousness about the nutritional and functional properties the rice bran is very important co-product. In this research article the methods were described regarding different chemicals with different concentration in controlling the activity of lipase enzyme and ultimately to maximize its oil recovery from the rice bran during 60 days of storage in a room temperature. The use of hydrochloric acid at concentration of about 30 ml/Kg helps greatly in controlling the lipase enzyme activity and reduces the % of FFA while the other chemicals used in this study (Phosphoric acid, Acetic acid, Sodium metabisulphite) failed to control in the rise of free fatty acid contents. The chemicals can be applied easily by sprinkling or spraying. This operation done on small rice bran lots through manual hand mixing. This method of chemical stabilization of rice bran is really a useful method in the rice mills where there is shortage of electricity or steaming facility.

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Section: Free Fatty Acid (Ffa) Status Of Rice Bransupporting

confidence: 88%

Inactivation of Lipase Enzyme by Using Chemicals to Maximize Rice Bran Shelf Life and its Edible Oil Recovery

Akhter¹,

Afzal²,

Haider³

et al. 2015

J Nutr Food Sci

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“…Serum levels of g-tocotrienol were measured using the Waters Alliance 2695 HPLC system (Waters, Milford, MA) as described (20). Data were pooled and analyzed using the naive pooled modeling approach (21).…”

Section: Pharmacokinetics Analysis Of G-tocotrienolmentioning

confidence: 99%

Vitamin E Isoform γ-Tocotrienol Downregulates House Dust Mite–Induced Asthma

Peh

Chang

et al. 2015

The Journal of Immunology

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Inflammation and oxidative damage contribute to the pathogenesis of asthma. Although corticosteroid is the first-line treatment for asthma, a subset of patients is steroid resistant, and chronic steroid use causes side effects. Because vitamin E isoform γ-tocotrienol possesses both antioxidative and anti-inflammatory properties, we sought to determine protective effects of γ-tocotrienol in a house dust mite (HDM) experimental asthma model. BALB/c mice were sensitized and challenged with HDM. Bronchoalveolar lavage (BAL) fluid was assessed for total and differential cell counts, oxidative damage biomarkers, and cytokine levels. Lungs were examined for cell infiltration and mucus hypersecretion, as well as the expression of antioxidants and proinflammatory biomarkers. Sera were assayed for IgE and γ-tocotrienol levels. Airway hyperresponsiveness in response to methacholine was measured. γ-Tocotrienol displayed better free radical–neutralizing activity in vitro and inhibition of BAL fluid total, eosinophil, and neutrophil counts in HDM mouse asthma in vivo, as compared with other vitamin E isoforms, including α-tocopherol. Besides, γ-tocotrienol abated HDM-induced elevation of BAL fluid cytokine and chemokine levels, total reactive oxygen species and oxidative damage biomarker levels, and of serum IgE levels, but it promoted lung-endogenous antioxidant activities. Mechanistically, γ-tocotrienol was found to block nuclear NF-κB level and enhance nuclear Nrf2 levels in lung lysates to greater extents than did α-tocopherol and prednisolone. More importantly, γ-tocotrienol markedly suppressed methacholine-induced airway hyperresponsiveness in experimental asthma. To our knowledge, we have shown for the first time the protective actions of vitamin E isoform γ-tocotrienol in allergic asthma.

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“…Tocopherol (α, γ and δ) analysis was performed by HPLC following the method described by Lee et al (2003). An HPLC (Sykam GmbH, Kleinostheim, Germany) equipped with an S-1121 dual piston solvent delivery system and S-3210 UV/Vis diode array detector was used.…”

Section: Tocopherol Contentmentioning

confidence: 99%

Effect of salinity on yield and quality of <i>Moringa oleifera</i> seed oil

Anwar¹,

Hussain²,

Ashraf³

et al. 2006

Grasas y Aceites

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RESUMENEfecto de la salinidad en la producción y calidad del aceite de semilla de Moringa oleifera.Ha sido examinada la variación en la producción y composición del aceite de semilla de Moringa oleifera extraído a partir de semillas cultivadas en Pakistan con diferente concentración salina en el cultivo. La producción obtenida por extracción con hexano fue de 33.50% y 32.79% respectivamente para las semillas cultivadas en áreas con alto y bajo contenido salino. El análisis de la varianza (ANOVA) reveló la inexistencia de diferencias significativas entre las caracterís-ticas físicas (índice de refracción a 40 °C, color y gravedad específica) y las químicas (índice de yodo, acidez libre, índi-ce de peróxidos, materia insaponificable, índice de saponificación, valores de dienos y trienos conjugados e índice de p-anisidina) de los aceites procedentes de ambas áreas. En el área de mayor salinidad las concentraciones de C 18:1 y C 16:0 de los aceites de semilla de Moringa oleifera fueron significativamente mayores (P< 0.001), mientras que el de C 14:0 fue significativamente menor. El análisis de tocoferoles demostró que las concentraciones de α-y δ-tocoferol eran superiores significativamente (P< 0.001) en los aceites procedentes de las áreas con alta salinidad, mientras que el contenido en γ-tocoferol fue superior significativamente (P< 0.001) en los procedentes del área de baja salinidad. Los resultados de este estudio muestran que la salinidad no afecta sobre el contenido en aceite de estas semillas. Sin embargo, puede afectar al contenido en tocoferoles y el perfil de ácidos grasos. PALABRAS-CLAVE:Calidad de aceite -Caracterización -Composición de ácidos grasos -Moringa oleifera -Salinidad -Tocoferoles. SUMMARY Effect of salinity on yield and quality of Moringa oleifera seed oilVariation in the yield and composition of Moringa oleifera (M. oleifera) seed oil from two differently adopted (non-saline and saline) provinces of Pakistan was examined. Hexaneextracted M. oleifera seeds from saline and non-saline areas contained 33.50% and 32.79% oil yield, respectively. The analysis of variance (ANOVA) revealed no significant differences in the physical (refractive index (40 o C), color and specific gravity (24 °C) p-anisidine value) characteristics of the oils obtained from both areas. The concentration of C 18:1 and C 16:0 was significantly (P < 0.001) higher whereas, that of C 14:0 was lower in M. oleifera seed oils from the saline area. A tocopherol analysis demonstrated the concentration of α-and δ-tocopherol of Moringa seed oils to be significantly (P < 0.001) higher from the saline area. Whereas, the contents of γ-tocopherol was found to be significantly (P < 0.001) higher in the Moringa seed oils native to the non-saline area.Results from the present study revealed that salinity did not affect the oil content of M. oleifera seeds. Nevertheless, it might affect the tocopherol and fatty acid profiles of M. oleifera seed oil.

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Simultaneous Determination of Tocotrienols, Tocopherols, Retinol, and Major Carotenoids in Human Plasma

Cited by 98 publications

References 36 publications

Inactivation of Lipase Enzyme by Using Chemicals to Maximize Rice Bran Shelf Life and its Edible Oil Recovery

Inactivation of Lipase Enzyme by Using Chemicals to Maximize Rice Bran Shelf Life and its Edible Oil Recovery

Vitamin E Isoform γ-Tocotrienol Downregulates House Dust Mite–Induced Asthma

Effect of salinity on yield and quality of <i>Moringa oleifera</i> seed oil

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