Simultaneous double cationic and anionic molecule separation from herring milt hydrolysate and impact on resulting fraction bioactivities

Durand, Rachel; Fraboulet, Erwann; Marette, André; Bazinet, Laurent

doi:10.1016/j.seppur.2018.08.017

Cited by 37 publications

(46 citation statements)

References 37 publications

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“…This could be explained by the presence of inhibitory molecules and/or the use of an insufficient dose for the decrease of inflammation in cellular models. Moreover, a previous study has shown that HMH2 separation by EDUF improved the glucose uptake in cultured cells by the concentration of bioactive compounds and the removal of inhibitors [43]. The interaction between peptides and PUFA found in these hydrolysates may also improve this bioactivity [43].…”

Section: Discussionmentioning

confidence: 96%

“…Moreover, a previous study has shown that HMH2 separation by EDUF improved the glucose uptake in cultured cells by the concentration of bioactive compounds and the removal of inhibitors [43]. The interaction between peptides and PUFA found in these hydrolysates may also improve this bioactivity [43]. Ouellet et al highlighted that PUFA consumed in the form of fish are more available than those added to the diet by showing that the interaction between cod protein and PUFA allowed an improvement of insulin sensitivity in insulin-resistant individuals [16].…”

Section: Discussionmentioning

confidence: 98%

“…HMH2 and HMH3 are the herring milt hydrolysate retentate after their separation by an ultrafiltration membrane, while HMH1 is the permeate. Hence, HMH1 is mostly composed of small-weight peptides and free amino acids, while HMH2 and HMH3 are composed of higher-weight peptides and lipids [22,43]. These differences in composition may explain the higher efficiencies of HMH2 and HMH3 treatments.…”

Section: Discussionmentioning

confidence: 99%

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Animal and Cellular Studies Demonstrate Some of the Beneficial Impacts of Herring Milt Hydrolysates on Obesity-Induced Glucose Intolerance and Inflammation

et al. 2020

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The search for bioactive compounds from enzymatic hydrolysates has increased in the last few decades. Fish by-products have been shown to be rich in these valuable molecules; for instance, herring milt is a complex matrix composed of lipids, nucleotides, minerals, and proteins. However, limited information is available on the potential health benefits of this by-product. In this context, three industrial products containing herring milt hydrolysate (HMH) were tested in both animal and cellular models to measure their effects on obesity-related metabolic disorders. Male C57Bl/6J mice were fed either a control chow diet or a high-fat high-sucrose (HFHS) diet for 8 weeks and received either the vehicle (water) or one of the three HMH products (HMH1, HMH2, and HMH3) at a dose of 208.8 mg/kg (representing 1 g/day for a human) by daily oral gavage. The impact of HMH treatments on insulin and glucose tolerance, lipid homeostasis, liver gene expression, and the gut microbiota profile was studied. In parallel, the effects of HMH on glucose uptake and inflammation were studied in L6 myocytes and J774 macrophages, respectively. In vivo, daily treatment with HMH2 and HMH3 improved early time point glycemia during the oral glucose tolerance test (OGTT) induced by the HFHS diet, without changes in weight gain and insulin secretion. Interestingly, we also observed that HMH2 consumption partially prevented a lower abundance of Lactobacillus species in the gut microbiota of HFHS diet-fed animals. In addition to this, modulations of gene expression in the liver, such as the upregulation of sucrose nonfermenting AMPK-related kinase (SNARK), were reported for the first time in mice treated with HMH products. While HMH2 and HMH3 inhibited inducible nitric oxide synthase (iNOS) induction in J774 macrophages, glucose uptake was not modified in L6 muscle cells. These results indicate that milt herring hydrolysates reduce some metabolic and inflammatory alterations in cellular and animal models, suggesting a possible novel marine ingredient to help fight against obesity-related immunometabolic disorders.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Animal and Cellular Studies Demonstrate Some of the Beneficial Impacts of Herring Milt Hydrolysates on Obesity-Induced Glucose Intolerance and Inflammation

et al. 2020

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“…Due to the important increase in fish consumption, by-products generated by industries have known an important increase over the last twenty years [1]. One major way of valorizating these by-products is based on their hydrolysis through the use of chemicals or enzymes.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Performance of Electrodialysis in the Removal of the Most Potent Odor-Active Compounds of Herring Milt Hydrolysate: Focus on Ion-Exchange Membrane Fouling and Water Dissociation as Limiting Process Conditions

et al. 2020

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Herring milt hydrolysate (HMH), like many fish products, presents the drawback to be associated with off-flavors. As odor is an important criterion, an effective deodorization method targeting the volatile compounds responsible for off-flavors needs to be developed. The potential of electrodialysis (ED) to remove the 15 volatile compounds identified, in the first part of this work, for their main contribution to the odor of HMH, as well as trimethylamine, dimethylamine and trimethylamine oxide, was assessed by testing the impact of both hydrolysate pH (4 and 7) and current conditions (no current vs. current applied). The ED performance was compared with that of a deaerator by assessing three hydrolysate pH values (4, 7 and 10). The initial pH of HMH had a huge impact on the targeted compounds, while ED had no effect. The fouling formation, resulting from electrostatic and hydrophobic interactions between HMH constituents and ion-exchange membranes (IEM); the occurrence of water dissociation on IEM interfaces, due to the reaching of the limiting current density; and the presence of water dissociation catalyzers were considered as the major limiting process conditions. The deaerator treatment on hydrolysate at pH 7 and its alkalization until pH 10 led to the best removal of odorant compounds.

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“…It is established that EDUF processes are highly effective in separating large molecules with biological activity[30,46,62]. Further, the approach uses no solvents or other chemicals that can add to the environmental footprint of the operation[63]. Hence, there is an incentive to further develop the process to make it suitable for the separation/fractionation of charged molecules of large size.…”

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confidence: 99%

Isolation of lactoferrin and immunoglobulins from dairy whey by an electrodialysis with filtration membrane process

Wang

Chen

Kentish

2020

Separation and Purification Technology

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Isolation of high value minor proteins such as lactoferrin and immunoglobulins from crude dairy streams is a major challenge for the dairy industry. Here we investigate an electrodialysis with filtration membrane (EDFM) approach to separate lactoferrin (LF) and immunoglobulins (Ig) from other dairy proteins. A polyvinyl alcohol (PVA) membrane is prepared using phase inversion in a coagulation bath with 80% ethanol to serve as the filtration membrane. A range of treatment times and two electric field strengths (38.5 and 77 V/cm) were then investigated within the electrical cell. The results show that the filtration membrane prepared in-house can offer strong rejection for LF and Ig while allowing a high flux of other proteins. The separation of LF and Ig from a simulated whey solution can be achieved. The use of a partially demineralised whey reduces protein loss, but there is a tradeoff between the energy consumption and the protein removal rate as the extent of this demineralisation is increased.

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Simultaneous double cationic and anionic molecule separation from herring milt hydrolysate and impact on resulting fraction bioactivities

Cited by 37 publications

References 37 publications

Animal and Cellular Studies Demonstrate Some of the Beneficial Impacts of Herring Milt Hydrolysates on Obesity-Induced Glucose Intolerance and Inflammation

Animal and Cellular Studies Demonstrate Some of the Beneficial Impacts of Herring Milt Hydrolysates on Obesity-Induced Glucose Intolerance and Inflammation

Assessment of the Performance of Electrodialysis in the Removal of the Most Potent Odor-Active Compounds of Herring Milt Hydrolysate: Focus on Ion-Exchange Membrane Fouling and Water Dissociation as Limiting Process Conditions

Isolation of lactoferrin and immunoglobulins from dairy whey by an electrodialysis with filtration membrane process

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