Simultaneous dual-targeted monitoring of breast cancer circulating miRNA via surface-enhanced Raman spectroscopy

A flap endonuclease 1-assisted universal viral nucleic acid sensing system using surface-enhanced Raman scattering

“…Electrochemical deposition of silver nanopillars was performed in a three-electrode system consisting of a platinum counter electrode and Ag/AgCl (1 M KCl) reference electrode at −2.0 V until −200 mC cm −2 of charge was passed. 40 After coating with platinum, the morphology of the formed nanopillar structures was observed using field-emission scanning electron microscopy at 10–15 kV (JSM 7610F-plus, JEOL, Tokyo, Japan).…”

“…39 Here, we present an optical biosensing platform based on FEN1-assisted cleavage activity and electrochemically deposited silver nanopillar structures for the highly sensitive and multiplexed detection of an avian influenza A (H9N2) virus. 40,41 We adopted a nanostructured solid platform with electrochemically grown silver nanopillars as a solid-state SERS substrate, displaying gaps of several nanometers between each adjacent pillar. With this SERS substrate, we could directly apply the sample solution to the substrate and obtain reproducible and uniform signals while ensuring easy substrate handling and storage compared to colloidal plasmonic nanoparticle dispersion.…”

Ratiometric Fluorescent Biosensor Based on Self-Assembled Fluorescent Gold Nanoparticles and Duplex-Specific Nuclease-Assisted Signal Amplification for Sensitive Detection of Exosomal miRNA

“…Therefore, researchers turned to developing novel detection strategies that are sensitive, selective, convenient (without complicated total RNA extraction steps), and easy to operate. These novel strategies are based on various functional nanomaterials and different principles such as fluorescence, surface-enhanced Raman spectroscopy, colorimetry, surface plasmon resonance, electrochemistry, photoelectrochemistry, and electrochemiluminescence. − Fluorescence-based methods are most commonly used for the determination of miRNAs due to their high sensitivity and simple operation . Various nanomaterials with fluorescence emission or quenching functions such as gold nanoparticles (Au NPs), Ti 3 C 2 T x , MoS 2 , g-C 3 N 4 , black phosphorus, graphene oxide, MnO 2 nanosheets, magnetic nanoparticles, metal-organic frameworks, and covalent organic frameworks have been explored for the detection of miRNAs. − Among them, Au NPs can effectively quench the fluorescence of fluorescent molecules through an energy transfer phenomenon.…”

Potential utility of miRNAs for liquid biopsy in breast cancer

“…Kim et al. successfully monitored the expression levels of miR-21 and miR-155 in the urinary samples of mice injected with BC cells ( 21 ). Hence, in this review, we summarize recent and relevant data on circulating miRNAs (c-miRNAs) for their potential role in the diagnostics of BC to become a part of the LB approach in clinics.…”

“…Additionally, Li et al have studied miRNAs in BC patients and demonstrated that 13 miRNAs were found to be differentially expressed in patients with metastatic BC (20). Kim et al successfully monitored the expression levels of miR-21 and miR-155 in the urinary samples of mice injected with BC cells (21). Hence, in this review, we summarize recent and relevant data on circulating miRNAs (c-miRNAs) for their potential role in the diagnostics of BC to become a part of the LB approach in clinics.…”