Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles

Wang, Jiuhai; Ali, Zeeshan; Wang, Nianyue; Liang, Wenbiao; Liu, Hongna; Fu, Li; Yang, Haowen; He, Lei; Nie, Libo; He, Nongyue; Li, Zhiyang

doi:10.1007/s11426-015-5483-x

Cited by 36 publications

(15 citation statements)

References 42 publications

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“…The application of magnetic nanoparticles for DNA and RNA isolation, also known Copyright: American Scientific Publishers as magnetic nucleic acids separation technology, has been widely accepted as a reliable method. [23][24][25][26][27][28][29][30][31][32] Automations based on this method had gained much attention. 17 20 33-35 However, those existing robotic solutions based on this technology are still far from perfect.…”

Section: Discussionmentioning

confidence: 99%

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Wu¹,

Hui²,

Zhu³

et al. 2016

j nanosci nanotechnol

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The use of magnetic nanoparticles (MNPs) in sample preparation has gained much attention. Easyto-handle property of MNPs has provided a great promising solution for lab automation. Laboratory robotics and workstations based on magnetic separation are in rapid development process, and increasing efforts are debited for their practical applications in clinical methods. To increase the processing throughput and reduce the risk of cross contamination, we developed a multifunctional module to optimize the workflow of magnetic nucleic acid separation in automated workstation. Three functions (magnetic separation, heating, and shaking) were elaborately combined into this device, by which the 96-well plate-based sample preparation can be completed on site without transferring the plate to other auxiliary modules. Test experiments were carried out on this platform. It was shown that this multifunctional device could cooperate well with our pipetting system, and this new platform exhibited its ability to meet the demands for high-throughput sample preparation.

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Section: Discussionmentioning

confidence: 99%

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Wu¹,

Hui²,

Zhu³

et al. 2016

j nanosci nanotechnol

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“…Each manufacturer, however, modifies their kit components to alter the binding and subsequent release of viral nucleic acid. This creates considerable variability in terms of binding capacity, contaminant removal and overall extraction efficacy [11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of five viral nucleic acid extraction kits for the efficient extraction of viral DNA and RNA from cell-free samples

Suliman¹

2019

Trends Med

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Extraction of viral nucleic acids from serum samples is widely used in diagnostic pathology tests. However, the heterogeneous nature of non-serum samples may contribute to variations in the yields of viral nucleic acids with different extraction methods and specimen types. Five different nucleic acid extraction methods were compared for optimal extraction of viral DNA or RNA from a prepared cell-free specimen. The viruses used were hepatitis B, hepatitis C. The specimen used was DNase and RNase free normal saline spiked with predefined quantities of viral DNA or RNA. The extraction protocol was carried out according to each manufacturer's recommendations. The extracted nucleic acids from each of the samples were amplified by PCR and compared to the original un-extracted standard control. The RTA kit as well as the Qiagen kit was shown to yield the highest amounts of viral nucleic acid.

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“…We therefore decorated the MWNTs with Fe 3 O 4 NPs to ensure a strong magnetic responsiveness and provide the targeted property for various applications. [44][45][46][47] Apart from providing stability, the key function of magnetic multi-walled carbon nanotubes (Fe 3 O 4 /MWNTs nanocomposites) is to enhance the transport of the CHE to the cancerous tissues through effectively passive targeting, and also to increase the sensitivity of various anticancer agents to multidrug resistance in tumors. Moreover, it's necessary to prepare various surface coated hydrophilic polymers in prolonging the in vivo circulation of drug when using intravenous injectable formulation.…”

Section: Introductionmentioning

confidence: 99%

Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

Cao¹,

Liang²,

Zhao³

et al. 2016

j biomed nanotechnol

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The work focused on manufacturing improved drug loaded multifunctional magnetic nanoparticles that can overcome the relative non-specificity and potential side-effects of some chemotherapeutic drugs to healthy tissues. A new drug delivery system, Chelerythrine (CHE) and Fe3O4 loaded multi-walled carbon nanotubes (Fe3O4/MWNTs-CHE nanocomposites) that can target hepatocytes when treating malignant tumors, was prepared through a simple adsorption method. The formulation and structure of the Fe3O4/MWNTs-CHE nanocomposites were characterized by vibrating sample magnetometer (VSM), Fourier Transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The cytotoxicity and anti-proliferation effect from the prepared nanocomposites were in vitro tested on human hepatocarcinoma HepG2 and normal liver LO2 cell lines. The results showed the saturated magnetization of Fe3O4/MWNTs-CHE nanocomposites could reach to 45.4O3 emu/g, and the in vitro CHE release behavior exhibited a biphasic release pattern. Moreover, the in vitro cytotoxicity studies revealed that the Fe3O4/MWNTs-CHE nanocomposites showed an efficient inhibition rate to HepG2 cell line and exhibited a lower cytotoxicity to LO2 cell line in comparison to the native CHE. Therefore, the multifunctional Fe3O4/MWNTs-CHE nanocomposites should be a useful and promising candidate for treatment of malignant tumors.

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Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles

Cited by 36 publications

References 42 publications

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Comparison of five viral nucleic acid extraction kits for the efficient extraction of viral DNA and RNA from cell-free samples

Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

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Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles

Cited by 36 publications

References 42 publications

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

Comparison of five viral nucleic acid extraction kits for the efficient extraction of viral DNA and RNA from cell-free samples

Chelerythrine and Fe3O4 Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

Contact Info

Product

Resources

About

Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy