Simultaneous Fluorescence Sensing of Cys and GSH from Different Emission Channels

Abstract

A chlorinated coumarin-hemicyanine dye with three potential reaction sites was exploited as fluorescent probe for biothiols. The Cys-induced substitution-rearrangement-cyclization, Hcy-induced substitution-rearrangement, and GSH-induced substitution-cyclizatioin cascades lead to the corresponding amino-coumarin, amino-coumarin-hemicyanine, thiol-coumarin with distinct photophysical properties, enabling Cys and GSH to be selectively detected from different emission channels at two different excitation wavelengths.

Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress

“…The integration of multiresponse to multi-species by a single probe can demonstrate the redox homeostasis process more clearly. [32][33][34][35][36][37][38][39] Moreover, the multiresponse probes can benefit from inaccurate calibration. And they can also avoid potobleaching rates of individual probes, uneven probe loading, nonhomogeneous distribution uncontrollable localization, larger invasive effects, metabolisms, and interference of spectral overlap.…”

Detection of biological thiols based on a colorimetric method

“…Several methods based on chromatography, electrophoresis, and fluorescent technology for biothiol detection have been reported, especially nanomaterial-based methods much facilitated by the development of the underlying nanotechnology (Lee et al, 2008;Hong et al, 2009;Yi et al, 2009;Shiu et al, 2010;Liu et al, 2014;Zhang et al, 2014;Zhu et al, 2014). Each method has its own advantage; however, pretreatments of the materials or expensive equipments are often necessary to obtain a good performance.…”

Selective fluorescence swing from cysteine to glutathione by switchover from solid to in situ probe in 100% water and bio-imaging studies for living species

“…Considering its basic physiological resemblance with higher animals including humans, it is the most suitable living species for bio-imaging [44]. Conventionally different cell lines are used to detect individual/total biothiols [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][31][32][33]. Here for the first time [51], we used multi-cellular organism C. elegans for sensing individual biothiols.…”

“…Moreover, interference due to high level of intercellular GSH often limits the precise detection of Cys and Hcy in biological systems [8][9][10][11]. Consequently, among a large number of biothiol sensors reported so far [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], only a few are capable of discriminating Cys, Hcy and GSH from one another and with exceptions [27][28][29], most of them remain associated with serious limitations, including the use of biotoxic organic/water mixed medium, poor biocompatibility and delayed response. Hence, there still remains a http://dx.doi.org/10.1016/j.snb.2014.…”