Simultaneous presence of bovine papillomavirus and bovine leukemia virus in different bovine tissues: in situ hybridization and cytogenetic analysis

Abstract

ABStRACt. Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate, especially when the DNA sequences are detected in tissues that are not specifically targeted by the virus. BPV and bovine leukemia virus (BLV) are clastogenic, causing chromosome aberrations in peripheral blood lymphocytes. In the present study, we investigated the simultaneous presence of DNA sequences of both viruses and the possibility of vertical transmission and compared the types of chromosome aberrations related to viral action. BPV 1, 2, and 4 DNA sequences were found in three females of the herd and in their offspring. BLV DNA sequences were not detected in their progeny. A newborn calf that was negative for BLV infection showed specific chromosome rearrangements possibly related to the effect of infection with BPV.

Can established cultured papilloma cells harbor bovine papillomavirus?

“…BPV DNA can occur in circulating lymphocytes of cattle, and latent BPV infection of lymphocytes was induced in experimental cattle (Stocco dos Santos et al, 1998). We have previously described the presence of BPV DNA in different tissues, not only epithelia, with evidence of virus activity (Yaguiu et al, 2006(Yaguiu et al, , 2008.…”

“…These primers are complementary to the L2 and L1 regions of the BPV genomes, for BPV1 and BPV2, respectively, and to the E7 region of BPV4, amplifying fragments of 301, 164 and 170 bp, respectively. The PCR protocol was according to Yaguiu et al (2008) using appropriate controls. As a control of the quality of DNA for PCR, the bovine β-globin gene was amplified using specific primers (Fw: 5'-AAC CTC TTT GTT CAC AAC CAG -3' and Rv: 5' -CAG ATG CTT AAC CCA CTG AGC -3') that yielded a 430-bp fragment, according to a protocol described by Yaguiu et al (2008).…”

“…Often, immunosuppression in cattle results from exposure to bracken fern: some animals become unable to reject the infection and succumb to widespread cutaneous or mucosal involvement (Borzacchiello and Roperto, 2008). These stages of papillomatosis are problematic leading to economic losses (Yaguiu et al, 2006(Yaguiu et al, , 2008.…”

“…Recently, the simultaneous presence of more than two viral agents infecting a single individual in the same tissue has been described (Yaguiu et al, 2006(Yaguiu et al, , 2008. The co-existence of different pathogens in the same tissue can result in deleterious interactions with the host cells and with the host chromatin (Yaguiu et al, 2008).…”

“…The co-existence of different pathogens in the same tissue can result in deleterious interactions with the host cells and with the host chromatin (Yaguiu et al, 2008).…”