Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds

Hwang, Ju Tae; Oh, Hyun-Mee; Kim, Mi Hwa; Jeong, Hyung Jae; Lee, Chul; Jang, Hyun Jae; Lee, Seung Woong; Park, Chan Sun; Rho, Mun Chual

doi:10.3390/molecules190710309

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…Mostly, HPLC detection coupled with different extraction methods and detection sources has been used for the analysis of oleanolic and ursolic acids in medicinal herbs and plants. [5][6][7][8][9][10] Some studies describe the application of gas 3,4 and liquid chromatography 11 coupled with a mass spectrometric detector, high-performance thin-layer chromatography (HPTLC), 1,12 supercritical uid chromatography with an evaporative light scattering detector (SFC-ELSD) 13 and a reversed phase-ultra ow liquid chromatography (RP-UFLC) 14 method for the determination of these triterpenoic acids.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric procedure for the determination of oleanolic and ursolic acids in plant extracts

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Voltammetric procedure for the determination of oleanolic and ursolic acids in plant extracts

et al. 2015

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“…Hovewer, cytotoxic effect against human monocyte THP1‐Blue of this compound has defined in the literature (Hwang et al . ). The anticancer activity of linarin which has several biological activities such as antianalgesic and anti‐inflammatory (Jordon‐Thaden and Louda ; Lim et al .…”

Section: Resultsmentioning

confidence: 97%

“…4B, the isolated compounds were shown not to have any considerable activity against Stigmasterol has not shown antiproliferative activity against C6 and HeLa cells. Hovewer, cytotoxic effect against human monocyte THP1-Blue of this compound has defined in the literature (Hwang et al 2014). The anticancer activity of linarin which has several biological activities such as antianalgesic and anti-inflammatory (Jordon-Thaden and Louda 2003;Lim et al 2008) was determined to be inactive against C6 and HeLa cells.…”

Section: Antiproliferative Activities Of Extracts and Isolated Compoundsmentioning

confidence: 99%

Studies on the Antioxidant and Antiproliferative Potentials ofCirsium arvensesubsp.vestitum

Demirtaş

Tüfekçi

Yağlıoğlu

et al. 2016

Journal of Food Biochemistry

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The antioxidant (DPPH, superoxide anion radical scavenging and FRAP) and antiproliferative activities against Vero cells (african green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) of Cirsium arvense (CA) of all parts [root (Kinghorn et al. 2004), stem (CAS), flower (CAF) and leaves] and isolated compounds were investigated in vitro. CAR was shown to have the highest antiproliferative activity against HeLa and C6 cell lines as compared to other parts. A flavon glucoside (linarin), a steroid glucoside (daucosterin), a steroid (stigmasterol) and a new benzymidazole molecule (E−6‐(3‐(5,6‐dimethoxy‐1H‐benzo[d] imidazol‐2‐yl)allyloxy)−5‐(hydroxymethyl)‐tetrahydro‐2H‐pyran‐2,3,4‐triol) were isolated from the CAR. The structures of these molecules were determined by the 13C (carbon) and 1H (proton)‐ Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FT‐IR), and Gas Chromatography‐Mass Spectrometry (GC‐MS) techniques. CAR for C6 cell line, CAS for HeLa and Vero cell line were observed the highest antiproliferative activity. In addition, CAR showed the stronger DPPH activity than α‐tocopherol and BHT at high concentrations. The FRAP and superoxide anion radical scavenging activities of CAR (Fr‐7) were found to be higher than BHT. Practical Applications Cirsium species are considered as agricultural weed among villagers in Turkey and are used as alternative medicinal plant for years. Studies have shown that these species are rich with regard to bioactive seconder metabolites. There is no phytochemical/bioactivity study about this plant until now. Our findings showed that the extracts of this plant possessed potent antioxidant activities. Four compounds, one of which is a new one to literature, were isolated and characterized. Finally, Cirsium arvense, being considered as an invader plant in agricultural fields, can be evaluated as a good source of antioxidant products.

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“…TLR signaling pathways include activation of NF-κB and MAPK signaling. Its inhibitory effect on NF-κB and MAPK signaling could inhibit TLR signaling; however, only TLR4 inhibitory effect was reported [27]. Based on the experimental results, synthetic compounds, such as the ssRNA (single-stranded RNA) mimic poly(I), were used to induce TLR3 activity and NF-κB-mediated signal transduction pathway activity to identify a therapeutic agent for TLR-mediated diseases.…”

Section: Discussionmentioning

confidence: 99%

Oleanolic Acid Acetate Exerts Anti-Inflammatory Activity via IKKα/β Suppression in TLR3-Mediated NF-κB Activation

et al. 2019

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Oleanolic acid acetate (OAA), a major triterpenoid compound of Vigna angularis (azuki bean, V. angularis), has been shown to downregulate inflammatory responses in macrophages. Here, we show the molecular basis for the effect of OAA on Toll-like receptor (TLR) downstream signaling. OAA treatment significantly inhibited the secretion of embryonic alkaline phosphatase (SEAP) induced by polyinosinic acid (poly(I), TLR3 ligand) in a dose-dependent manner and without cytotoxicity in THP1-XBlue cells. In addition, OAA downregulated the gene expression of poly(I) induced pro-inflammatory cytokines and chemokines genes such as MCP-1, IL-1β, IL-8, VCAM-1 and ICAM-1. Furthermore, we found that the inhibition activity of OAA was accompanied by decreased activation of not only nuclear factor-kappa B (NF-κB) signaling but also mitogen-activated protein kinase (MAPK) signaling upon stimulation with the TLR3 agonist. Interestingly, the interaction of OAA with IκB kinase α/β (IKKα/β) strongly attenuated the production of certain proteins and inflammatory cytokines in the TLR3 signaling pathway, such as nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkBα), extracellular regulated kinases (ERK), and p38, in an in vitro model. The action of OAA was regulated by TLR3, demonstrating that TLR3 plays a critical role in mediating the physiologically-relevant anti-inflammatory action of OAA and that the interaction with IKKα/β is modulated through TLR3. These results reveal new insight into the understanding of the regulatory mechanisms of the downstream TLR3 signaling pathway and consequent inflammatory responses that are involved in the development and progression of inflammatory diseases.

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Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds

Cited by 8 publications

References 12 publications

Voltammetric procedure for the determination of oleanolic and ursolic acids in plant extracts

Voltammetric procedure for the determination of oleanolic and ursolic acids in plant extracts

Studies on the Antioxidant and Antiproliferative Potentials ofCirsium arvensesubsp.vestitum

Oleanolic Acid Acetate Exerts Anti-Inflammatory Activity via IKKα/β Suppression in TLR3-Mediated NF-κB Activation

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