Simultaneous quantitation of metformin and sitagliptin from mouse and human dried blood spots using laser diode thermal desorption tandem mass spectrometry

Swales, John G.; Gallagher, Richard T.; Denn, Mark; Peter, Raimund M.

doi:10.1016/j.jpba.2011.02.030

Cited by 68 publications

(44 citation statements)

References 12 publications

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“…6 A survey of the literature revealed some HPLC and LC-MS/MS methods for analysis of MET alone [7][8][9][10][11][12][13] and in combination with other drugs. However, quite a few HPLC methods have been reported for simultaneous determination of MET with SIT [14][15][16][17][18][19][20][21][22] or VLD [23][24][25][26][27][28] or LIN 29,30 in formulations, but, no simple and rapid method is yet established for simultaneous determination of Metformin, Sitagliptin, Vildagliptin and Linagliptin using single analytical procedure. Development of single analytical procedure has an advantage of analyzing all drugs alone or in combination using same column and single mobile phase.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

IJPER

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Objective: A simple, rapid and validated liquid chromatographic method has been developed for the simultaneous determination of three novel Gliptins namely Vildagliptin (VLD), Sitagliptin (SIT) and Linagliptin (LIN) in their binary mixture with Metformin (MET). Methodology: The separation was performed on fast monolithic column using isocratic, mobile phase consisting of mixture of sodium dihydrogen phosphate, Sodium dedosyl sulphate and acetonitrile. The flow rate was 2.5 mL/min and UV detection for MET, VLD and SIT was conducted at 208 nm, whereas, that of MET and LIN was at 228 nm. The incipiently developed method was subjected for validation according to ICH guidelines. Results: The calibration curves of analytes showed good correlation (r2>0.999) over a concentration range 10-100 μg/mL and 50-400 μg/mL for Metformin, 1-10 μg/mL for Vildagliptin & Sitagliptin and 0.25-2.0 μg/mL for Linigliptin. Conclusion: All three tablet formulations were assayed with accuracy and precision and without interference from excipients. The method is also stability indicating with respect to Linagliptin.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

IJPER

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“…Literature review revealed the determination of sitagliptin in dosage form either alone or combined form by UV spectrophotometry [13][14][15] , RP-HPLC 5,16-21 , UPLC 22 , tandem mass spectrometry 23,24 and capillary electrophoresis. 25 However, UV spectrophotometry is the simplest technique but it is deficient in accuracy and precision and needs relatively higher amount of analytes for detection.…”

Section: Sitagliptin Chemically [(R)mentioning

confidence: 99%

Quantitation of Sitagliptin in Drug Product by Validated Reversed Phase Liquid Chromatographic Technique

Sultana

Hossain

Islam

et al. 2018

Dhaka Univ. J. Pharm. Sci

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ABSTRACT:A novel reversed phase ultra-high performance liquid chromatographic (RP-UHPLC) method was developed for the estimation of sitagliptin in pharmaceutical dosage form. Separation was done by a X-bridge C18 column (4.6 i.d.× 150 mm, 5 μm particle size) with a flow rate of 1 ml/min using phosphate buffer (pH 6) and acetonitrile (70:30, v/v) as mobile phase at 268 nm using photodiode array plus (PDA+) detector. The retention time was found at 4.607 min. The developed method was validated as per the requirements of ICH-Q2B guidelines for specificity, system suitability, linearity, precision, accuracy, sensitivity and robustness. The linear regression analysis data for the linearity plot showed correlation coefficient values of 0.999 with LOD value of 0.06 µg/ml and LOQ of 0.225 µg/ml. The relative standard deviation (%RSD) for inter-day and intra-day precision was not more than 2.0%. The method was found to be accurate with percentages recovery of 98.50±0.03 to 99.70±0.05 and the % RSD was less than 2. The results showed that the proposed method is highly convenient for routine analysis of sitagliptin.

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“…Chemically it is known as (2R) , RP-HPLC 9-15 HPTLC [16][17] and LC-MS [18][19][20] alone or in combination with other agents. Because of cost-effective and minimal maintenance, UV spectrophotometry is always preferred at small scale industries.…”

Section: -3mentioning

confidence: 99%

Uv Spectrophotometric Method Development and Validation for Estimation of Sitagliptin Phosphate in Bulk and Tablet Dosage Form by Absorption Ratio and Area Under the Curve Method

Chavhan¹,

Kadam²

2013

J Biol Sci Opin

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To develop two simple UV spectrophotometric methods for simultaneous estimation of Sitagliptin INTRODUCTIONSitagliptin phosphate (STG) is the first of a new class of drugs i.e. oral dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of type II diabetes which improves glycaemic control by inhibiting DPP-4 inactivation of the increatin hormones glucagon-like peptide-1 (GLP-1) and glucosedependent insulin tropic polypeptide (GIP). This increases active increatin and insulin levels and decreases glucagon levels and post-glucose-load glucose excursion.

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Simultaneous quantitation of metformin and sitagliptin from mouse and human dried blood spots using laser diode thermal desorption tandem mass spectrometry

Cited by 68 publications

References 12 publications

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Quantitation of Sitagliptin in Drug Product by Validated Reversed Phase Liquid Chromatographic Technique

Uv Spectrophotometric Method Development and Validation for Estimation of Sitagliptin Phosphate in Bulk and Tablet Dosage Form by Absorption Ratio and Area Under the Curve Method

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