Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry

Abstract

Pentodeuterated 4-hydroxymethamphetamine (HMAMP-d5), 1-(4-hydroxyphenyl)-2-(methyl-d3-amino)-propane-1,2-d2 of high quality was prepared and proved to be a most useful internal standard for quantitative analyses of 4-hydroxymethamphetamine (HMAMP), a main metabolite of methamphetamine (MAMP). Highly reliable results were obtained by gas chromatography/mass spectrometry. Simultaneously we determined also MAMP, amphetamine (AMP), 4-hydroxyamphetamine (HAMP), and HMAMP in body fluids, using two internal standards, HMAMP-d5 for HMAMP and HAMP and pentodeuterated methamphetamine, 1-phenyl-2-(methyl-d3-amino)-propane-1,2-d2, for MAMP and AMP.

Rapid GC–MS analysis of methamphetamine and its metabolites in urine—application of a short narrow-bore capillary column to GC–MS☆

“…Amphetamine (AMP) sulfate was obtained as official standards for criminal drug testing from the National Institute of Health Sciences in the Ministry of Health, Labour and Welfare of Japan. 4-hydroxymethamphetamine (4-HMAMP) was prepared as described in our previous report [7]. Phentermine (␣, ␣-dimethylphenethylamine) hydrochloride as an internal standard (IS) and heptafluoro-n-butyryl (HFB) chloride were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA).…”