Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Zhao, Xue; Jin, Xindi; Lin, Zhang; Guo, Qi; Liu, Bin; Yuan, Yahong; Yue, Tianli; Zhao, Xubo

doi:10.3390/foods10112738

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…Compared to the native product, the reduction in A w values therefore represent one of the main parameters of ensuring the inhibition of the growth of organisms, consequently giving stability to the food from both a microbiological, enzymatic, and chemical point of view. Foodstuffs of vegetable origin such as cereals and derivatives, seeds, dried fruit, coffee, cocoa, herbs, and spices are easily attacked by molds, which in particular conditions of temperature and humidity, can produce secondary potentially dangerous metabolites such as mycotoxins [ 14 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Mineral and Microbiological Analysis of Spices and Aromatic Herbs

Cicero

Gervasi

Durazzo³

et al. 2022

Foods

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Spices and aromatic herbs have always had great historical importance in human nutrition. Their use has been documented for centuries as a rich source of bioactive compounds; they have been used for their health benefits and also for flavoring or coloring food. However, despite the many health properties linked to the use of spices and aromatic herbs, these can represent biological hazards and can contain chemical substances of concern. Certainly, monitoring potential health hazards in spices and aromatic herbs includes microbiological safety and also the content of inorganic substances: both represent a key step. This research aims at monitoring the compliance of various spices and aromatic herbs from a non-European country market (namely: black cumin seeds, Iranian Tokhme Sharbati, clove buds, Shahjeera, Abbaszadeh saffron, organic fenugreek, whole black pepper, cinnamon, Abthul Ahmar (Asario), Ajwan seeds, whole coriander seeds, black sesame seeds, Sabja seeds) with the current European Union (EU) and WHO regulations, when available, regarding mineral and microbiological parameters. In particular, microbiological assays using rapid and conventional methods, and trace mineral determination by inductively coupled plasma mass spectrometry (ICP-MS) were performed. Results show the safety of the tested spices, given that the microbiological parameters were within the legal microbiological criteria set by the European Commission Regulation (EC) No. 2073/2005 and its amendment Regulation (EC) No. 1441/2007. With reference to potentially toxic Cd, Pb, As, Hg, these were within the limits set by the European Commission Regulation (EC) No. 1881/2006 and its amendments, Regulation (EU) No. 1317/2021 and Regulation (EU) No. 1323/2021, and WHO. According to EU regulations, for Pb content, 2 samples out of 16 showed values different from the set limits.

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Section: Introductionmentioning

confidence: 99%

Mineral and Microbiological Analysis of Spices and Aromatic Herbs

Cicero

Gervasi

Durazzo³

et al. 2022

Foods

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“…Regarding OTA analysis, our results highlighted contamination levels exceeding the upper limits set by EOSQC in spices and Gramineae. Typically, tropical settings with high temperatures, humidity, and rainfall conditions used to grow spices are ideal for the growth of microorganisms (Zhao et al 2021 ). These conditions are also crucial factors in producing OTA during the time of harvest, drying, and storage of Gramineae and cereals (Li et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of ELISA and immunoaffinity fluorometric analytical tools of four mycotoxins in various food categories

Boshra,

El-Housseiny,

Farag

et al. 2023

AMB Expr

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Mycotoxins (MTs) are secondary toxic metabolites that can contaminate food, impacting quality and safety, leading to various negative health effects and serious pathological consequences conferring urgent need to evaluate and validate the currently standard methods used in their analysis. Therefore, this study was aimed to validate ELISA and VICAM immunoaffinity fluorometric, the two common methods used to monitor the level of MTs according to the Egyptian Organization for Standardization and Quality Control. A total of 246 food samples were collected and tested for Aflatoxins (196 samples), Ochratoxin A (139), Zearalenone (70), and Deoxynivalenol (100) using both analytical methods. Results showed that aflatoxins exceeded limits in 42.9, 100, and 13.3% of oily seeds, dried fruits, and chili and spices, respectively. For ochratoxin A, 3.9% of Gramineae and 8% of spices and chili (locally sourced) exceeded the limits, while 17.6% of imported pasta and noodles exceeded the limits for deoxynivalenol. Significant differences for the aflatoxins and ochratoxin A detection among different categories of chocolate, dried fruits, and oily seeds (p-value < 0.05). No zearalenone contamination was detected in the exported, imported, and locally sourced categories. No deoxynivalenol contamination was detected in the tested Gramineae category. In contrast, for pasta and noodles, the imported samples exhibited the highest contamination rate (above the upper limit of 750 µg/kg) with 17.6% of the samples testing positive for deoxynivalenol with no significant difference among different sample categories of Gramineae, pasta, and noodles (p-value > 0.05). In conclusion, our study found no significant differences between the ELISA and immunoaffinity fluorometric analysis in the detection of the respective MTs in various food categories and therefore, they can substitute each other whenever necessary. However, significant differences were observed among different food categories, particularly the local and imported ones, highlighting the urgent need for strict and appropriate control measures to minimize the risk of MTs adverse effects.

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“…There are some reports that introduced lateral flow assay (LFAs)-based optical biosensors (Anfossi et al, 2012;Anfossi et al, 2013;Moon et al, 2013;Zhou et al, 2016b;Jiang et al, 2017;Ren et al, 2018;Velu and DeRosa, 2018;Oh et al, 2019;Hao et al, 2021;Zhao et al, 2021).…”

Section: Ii) Optical Aptasensorsmentioning

confidence: 99%

Aptamer based detection and separation platforms for ochratoxin A: A systematic review

TORABI¹,

Rezvanipour²,

GOUVARCHINGHALEH³

et al. 2022

Biocell

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Ochratoxin A (OTA), one of the most dangerous mycotoxins for human health, has been subjected to numerous studies for separation and detection in minimal amounts. Aptamers as novel recognition elements have been employed to fabricate ultrasensitive biosensors for the detection of OTA and designing delicate analytical tools. This review attempted to comprehensively examine all reported aptamer-based detection and separation platforms for ochratoxin. The most relevant databases were considered to discover all specific aptamers for dealing with OTA.Aptamer-based detection and separation devices specified for OTA were searched for, analyzed, discussed, and classified based on their specifications. The optical aptasensors have gathered a higher interest than electrochemical aptasensors, which can achieve a lower limit of detections. Moreover, some extraction platforms based on these aptamers were also found. However, aptamer-based devices seem to have some challenges in their application.

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Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Cited by 14 publications

References 25 publications

Mineral and Microbiological Analysis of Spices and Aromatic Herbs

Mineral and Microbiological Analysis of Spices and Aromatic Herbs

Evaluation of ELISA and immunoaffinity fluorometric analytical tools of four mycotoxins in various food categories

Aptamer based detection and separation platforms for ochratoxin A: A systematic review

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