Simultaneous Removal of the Antimicrobial Activity and Toxicity of Sulfamethoxazole and Trimethoprim by White Rot Fungi

Araújo, Caroline Aparecida Vaz de; Maciel, Giselle Maria; Rodrigues, Elidiane Andressa; Silva, Larissa Lachi; Oliveira, Roselene Ferreira; Brugnari, Tatiane; Peralta, Rosane Marina; Souza, Cristina Giatti Marques de

doi:10.1007/s11270-017-3525-z

Cited by 18 publications

(8 citation statements)

References 38 publications

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“…In contrast to what was often observed in pollutant exposed microbial communities and/or populations (e.g., da Silva Coelho et al, 2010; Artigas et al, 2017; de Araujo et al, 2017; Singh et al, 2017), the laccase activity decreased in the presence of nicosulfuron, showing a reduction of about 60% activity, whatever the lifestyle (Figure 5). The obtained results highlight that laccase activity responses to xenobiotic contamination are probably molecule-specific (Baldrian, 2006).…”

Section: Discussioncontrasting

confidence: 65%

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

et al. 2018

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Nicosulfuron is a selective herbicide belonging to the sulfonylurea family, commonly applied on maize crops. Its worldwide use results in widespread presence as a contaminant in surface streams and ground-waters. In this study, we isolated, for the first time, the Plectosphaerella cucumerina AR1 nicosulfuron-degrading fungal strain, a new record from Alnus leaf litter submerged in freshwater. The degradation of nicosulfuron by P. cucumerina AR1 was achieved by a co-metabolism process and followed a first-order model dissipation. Biodegradation kinetics analysis indicated that, in planktonic lifestyle, nicosulfuron degradation by this strain was glucose concentration dependent, with a maximum specific degradation rate of 1 g/L in glucose. When grown on natural substrata (leaf or wood) as the sole carbon sources, the Plectosphaerella cucumerina AR1 developed as a well-established biofilm in 10 days. After addition of nicosulfuron in the medium, the biofilms became thicker, with rising mycelium, after 10 days for leaves and 21 days for wood. Similar biofilm development was observed in the absence of herbicide. These fungal biofilms still conserve the nicosulfuron degradation capacity, using the same pathway as that observed with planktonic lifestyle as evidenced by LC-MS analyses. This pathway involved first the hydrolysis of the nicosulfuron sulfonylurea bridge, leading to the production of two major metabolites: 2-amino-4,6-dimethoxypyrimidine (ADMP) and 2-(aminosulfonyl)-N,N-dimethyl-3-pyridinecarboxamide (ASDM). One minor metabolite, identified as 2-(1-(4,6-dimethoxy-pyrimidin-2-yl)-ureido)-N,N-dimethyl-nicotinamide (N3), derived from the cleavage of the C-S bond of the sulfonylurea bridge and contraction by elimination of sulfur dioxide. A last metabolite (N4), detected in trace amount, was assigned to 2-(4,6-dimethoxy-pyrimidin-2-yl)-N,N-dimethyl-nicotinamide (N4), resulting from the hydrolysis of the N3 urea function. Although fungal growth was unaffected by nicosulfuron, its laccase activity was significantly impaired regardless of lifestyle. Leaf and wood surfaces being good substrata for biofilm development in rivers, P. cucumerina AR1 strain could thus have potential as an efficient candidate for the development of methods aiming to reduce contamination by nicosulfuron in aquatic environments.

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Section: Discussioncontrasting

confidence: 65%

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

et al. 2018

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“…In this respect, the use of white rot fungi and their ligninolytic enzymes has emerged as an interesting alternative to bacterial biodegradation (Čvančarová et al 2015;Lucas et al 2016;Navada and Kulal 2019). Several biotechnological approaches have been successfully tested to determine the biodegradation of sulfonamides by white rot fungi (Rodríguez-Rodríguez et al 2012;de Araujo et al 2017), using free or immobilized laccase enzymes (Schwarz et al 2010;Rahmani et al 2015;García-Delgado et al 2018;Alharbi et al 2019) or using spent mushroom substrate (SMS) (Chang et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

An assessment of Pleurotus ostreatus to remove sulfonamides, and its role as a biofilter based on its own spent mushroom substrate

Mayans

Camacho-Arévalo

García‐Delgado

et al. 2020

Environ Sci Pollut Res

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A double strategy based on the removal of sulfonamide antibiotics by Pleurotus ostreatus and adsorption on spent mushroom substrate was assessed to reclaim contaminated wastewater. P. ostreatus was firstly tested a in liquid medium fortified with five sulfonamides: sulfamethoxazole, sulfadiazine, sulfathiazole, sulfapyridine and sulfamethazine, to evaluate its capacity to remove them, to test for any adverse effects on fungal growth and for any reduction in residual antibiotic activity. P. ostreatus was effective in removing sulfonamides up to 83 to 91 % of the applied doses over 14 days. The antibiotic activity of the sulfonamide residues was reduced by 50 %. Sulfamethoxazole transformation products by laccase were identified and the degradation pathway was proposed. In addition, P. ostreatus growth on a semi-solid medium of spent mushroom substrate and malt extract agar was used to develop a biofilter for the removal of sulfonamides from real wastewater. The biofilter was able to remove more than 90% of the sulfonamide concentrations over 24 hours by combining adsorption and biodegradation mechanisms.

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“…Similarly, Cvancarova et al (2015) reported the elimination of ciprofloxacin, ofloxacin and norfloxacin by Irpex lacteus and T. versicolor. Pleurotus ostreatus degraded oxytetracycline (Migliore et al, 2012), sulfamethoxazole, and trimethoprim (De Araujo et al, 2017). However, under author knowledge, the biotransformation of IP by WRF has not been reported.…”

mentioning

confidence: 94%

“…A novel alternative to physical and chemical treatments are biological processes using white-rot fungi (WRF) and their non-specific and extracellular ligninolytic enzymes. This method, which could be implemented in WWTPs as secondary or tertiary treatments, appears as a viable option for the removal of antibiotics (Čvančarová et al, 2015;De Araujo et al, 2017;Tortella et al, 2013). In addition to high catabolic degradative potential, processes based on WRF are a low cost and an environmentally friendly method (Osma et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal

Copete-Pertuz

Plácido

Serna-Galvis

et al. 2018

Science of The Total Environment

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In this work, Leptosphaerulina sp. (a Colombian native fungus) significantly removed three Isoxazolyl-Penicillin antibiotics (IP): oxacillin (OXA, 16000 μg L), cloxacillin (CLX, 17500 μg L) and dicloxacillin (DCX, 19000 μg L) from water. The biological treatment was performed at pH 5.6, 28 °C, and 160 rpm for 15 days. The biotransformation process and lack of toxicity of the final solutions (antibacterial activity (AA) and cytotoxicity) were tested. The role of enzymes in IP removal was analysed through in vitro studies with enzymatic extracts (crude and pre-purified) from Leptosphaerulina sp., commercial enzymes and enzymatic inhibitors. Furthermore, the applicability of mycoremediation process to a complex matrix (simulated hospital wastewater) was evaluated. IP were considerably abated by the fungus, OXA was the fastest degraded (day 6), followed by CLX (day 7) and DCX (day 8). Antibiotics biodegradation was associated to laccase and versatile peroxidase action. Assays using commercial enzymes (i.e. laccase from Trametes versicolor and horseradish peroxidase) and inhibitors (EDTA, NaCl, sodium acetate, manganese (II) ions) confirmed the significant role of enzymatic transformation. Whereas, biomass sorption was not an important process in the antibiotics elimination. Evaluation of AA against Staphylococcus aureus ATCC 6538 revealed that Leptosphaerulina sp. also eliminated the AA. In addition, the cytotoxicity assay (MTT) on the HepG2 cell line demonstrated that the IP final solutions were non-toxic. Finally, Leptosphaerulina sp. eliminated OXA and its AA from synthetic hospital wastewater at 6 days. All these results evidenced the potential of Leptosphaerulina sp. mycoremediation as a novel environmentally friendly process for the removal of IP from aqueous systems.

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Simultaneous Removal of the Antimicrobial Activity and Toxicity of Sulfamethoxazole and Trimethoprim by White Rot Fungi

Cited by 18 publications

References 38 publications

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

Nicosulfuron Degradation by an Ascomycete Fungus Isolated From Submerged Alnus Leaf Litter

An assessment of Pleurotus ostreatus to remove sulfonamides, and its role as a biofilter based on its own spent mushroom substrate

Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal

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