Simultaneous Separation and Quantitative Determination of Monosaccharides, Uronic Acids, and Aldonic Acids by High Performance Anion-Exchange Chromatography Coupled With Pulsed Amperometric Detection in Corn Stover Prehydrolysates

Wang, Xing; Xu, Yong; Li, Fan; Yong, Qiang; Yu, Shiyuan

doi:10.15376/biores.7.4.4614-4625

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…The presence of spontaneous COL assembly with GlucA is potentially due to an interaction between the positively charged COL and the negatively charged carboxylate group of GlucA. [51][52][53] This electrostatic interaction may also account for the shorter lag time for 0.5 M compared with 0.25 M GlucA, as well as the lessened effect on delaying the kinetics. Although these aggregates may be seeding COL nucleation, the rate of kinetic assembly was still delayed compared with the COL-only condition, and the steady-state absorbance was still increased.…”

Section: Influence Of Monosaccharides On Col Polymerizationmentioning

confidence: 99%

A Role for Monosaccharides in Nucleation Inhibition and Transport of Collagen

et al. 2020

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Background: Collagenous tissues are composed of precisely oriented, tightly packed collagen fibril bundles to confer the maximal strength within the smallest volume. While this compact form benefits mobility, it consequentially restricts vascularity and cell density to a minimally viable level in some regions. These tissues reside in a homeostatic state with an unstable equilibrium, where perturbations to structure or molecular milieu cause descension into a long-term compromised state. Several studies have shown that glycosaminoglycans are key molecules required for healthy tissue maintenance. Our long-term goal is to determine if glycosaminoglycans serve a critical function of stabilizing soluble monomeric collagen in the interstitial fluid that bathes tissue for immediate availability in tissue development and repair in vivo. Materials and Methods: To test glycosaminoglycan and collagen interactions at the most fundamental level, we have explored the effect of the monosaccharides that populate the glycosaminoglycans of the extracellular matrix on collagen assembly kinetics, pre-established matrix stability, and collagen incorporation into a preassembled matrix. Results: Results showed that monosaccharides increased the threshold concentration required for spontaneous polymerization by at least three orders of magnitude. When the monosaccharides were introduced to a pre-existing collagen network, fibrillar dissociation was undetectable. Fluorescent-labeling studies illustrated that in the presence of the saccharide solution, soluble collagen maintains the functional capacity to integrate into a pre-existing network. Conclusion: This work demonstrates a feasible role for glycosaminoglycans in supporting tissue remodeling and highlights the potential importance of age-related deterioration of glycosaminoglycan biosynthesis in reference to the homeostasis of collagen-based tissues.

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Section: Influence Of Monosaccharides On Col Polymerizationmentioning

confidence: 99%

A Role for Monosaccharides in Nucleation Inhibition and Transport of Collagen

et al. 2020

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“…The mobile phase consisted of degassed solution A (200 mmol L −1 NaOH) and solution B (water) at flow rate of 0.5 mL min −1 . The following gradient elution program was used: at t = 0 min, 10:90 (solution A:solution B); at t = 10 min, 100:0; at t = 25 min, 100:0; t = 25.1 min, 10:90; t = 30 min, 10:90 …”

Section: Identification Of Fa By Gc‐msmentioning

confidence: 99%

Gluconobacter oxydans (ATCC 621H) catalyzed oxidation of furfural for detoxification of furfural and bioproduction of furoic acid

Zhou

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND The bacterial strain Gluconobacter oxydans (ATCC 621H) was investigated for its potential biodetoxification properties through conversion of furfural to 2‐furoic acid (FA). Furfural is a common byproduct of lignocellulose feedstock pretreament processing and it is a putative inhibitor of several important microbial pathways. RESULTS Both furfural and furfuryl alcohol exhibit significant substrate inhibition to biooxidation. To overcome this inhibition, a fed‐batch bioprocess was developed to convert furfural and furfuryl alcohol to the low‐toxic FA product. The optimized fed‐batch bioprocess gave FA concentrations exceeding 40 g L−1 with a productivity of 0.167 g L−1 h−1 and a yield of nearly 100%. In order to improve productivity, the whole cell catalysis was carried out in a compressed oxygen supply‐sealed and stirred tank reactor (COS‐SSTR). Similar to the flask studies, 38.18 g L−1 FA was obtained in 24 h. CONCLUSION Gluconobacter oxydans was found to oxidize both furfural and furfuryl alcohol to a single product, FA, with a yield close to 100%. Unlike other microorganisms, this strain does not have a metabolic system to further degrade FA, demonstrating its potential to produce this useful product from renewable resources. © 2016 Society of Chemical Industry

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“…2). At the same time, there are various columns routinely suitable for determination of xylose and glucose on the HPAEC-PAD system, e.g., the columns of CarboPac PA1, PA10, and PA20 (Wang et al 2012). In addition, the HPLC system is also commonly used for xylose and glucose detection on the column of Bio-Rad HPX-87P or 87H.…”

Section: Simultaneous Separation and Determination Of The Xos And Cosmentioning

confidence: 99%

Simultaneous Separation and Quantification of Linear Xylo- and Cello-Oligosaccharides Mixtures in Lignocellulosics Processing Products on High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

Xu¹,

Fan²,

Wang³

et al. 2013

BioResources

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A simple one-step method was developed for rapid separation and quantification of the linear xylo-oligosaccharides (XOS) and cellooligosaccharides (COS) mixtures by using high-performance anionexchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). By exploiting small ion-exchange behavioral differences of various oligosaccharide components on the CarboPac PA200 column, a two-stage binary gradient elution program of NaOAc-NaOH solution was established. Subsequently, nine linear oligomers were separated simultaneously and readily within 30 min, in the order of: xylobiose, cellobiose, xylotriose, xylotetraose, cellotriose, xylopentaose, cellotetraose, xylohexaose, and cellopentaose. The method was applied successfully in the analysis and determination of different lignocellulosics processing products. The system provides a convenient and powerful analytical tool for technical research and development on polysaccharide components bioconversion in lignocellulosic biomass processing.

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Simultaneous Separation and Quantitative Determination of Monosaccharides, Uronic Acids, and Aldonic Acids by High Performance Anion-Exchange Chromatography Coupled With Pulsed Amperometric Detection in Corn Stover Prehydrolysates

Cited by 8 publications

References 19 publications

A Role for Monosaccharides in Nucleation Inhibition and Transport of Collagen

A Role for Monosaccharides in Nucleation Inhibition and Transport of Collagen

Gluconobacter oxydans (ATCC 621H) catalyzed oxidation of furfural for detoxification of furfural and bioproduction of furoic acid

Simultaneous Separation and Quantification of Linear Xylo- and Cello-Oligosaccharides Mixtures in Lignocellulosics Processing Products on High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

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