Simultaneous Spectrophotometric Determination of Tramadol and Acetaminophen in Pharmaceutical Formulations Using H-Point Standard Addition Method

Zarei, Ali Reza; Mardi, Kobra; Chalavi, Soheila

doi:10.12816/0000362

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Fluoroquinolones were determined in the environmental water samples [ 9 ], plasma, and urine [ 10 ]. CPE was also applied to determine cetylpyridinium chloride in the pharmaceutical formulations [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The Chemometric Evaluation of the Factors Influencing Cloud Point Extraction for Fluoroquinolones

Michałowska,

Kupczyk,

Czyrski

2023

Pharmaceutics

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This study aimed to analyze the factors that impact the cloud point extraction of ciprofloxacin, levofloxacin, and moxifloxacin. The following independent variables were analyzed: Triton X-114 concentration, NaCl concentration, pH, and incubation temperature. The dependent variable studied was recovery. A central composite design model was used. The applied quantitation method was HPLC. The method was validated for linearity, precision, and accuracy. The results underwent ANOVA® analysis. The polynomial equations were generated for each analyte. The response surface methodology graphs visualized them. The analysis showed that the factor most affecting the recovery of levofloxacin is the concentration of Triton X-114, while the recovery of ciprofloxacin and moxifloxacin is most affected by pH value. However, the concentration of Triton X-114 also plays an important role. The optimization resulted in the following recoveries: for ciprofloxacin, 60%; for levofloxacin, 75%; and for moxifloxacin, 84%, which are identical to those estimated with regression equations—59%, 74% and 81% for ciprofloxacin, levofloxacin, and moxifloxacin, respectively. The research confirms the validity of using the model to analyze factors affecting the recovery of the analyzed compounds. The model allows for a thorough analysis of variables and their optimization.

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Section: Introductionmentioning

confidence: 99%

The Chemometric Evaluation of the Factors Influencing Cloud Point Extraction for Fluoroquinolones

Michałowska,

Kupczyk,

Czyrski

2023

Pharmaceutics

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“…The drug selectively blocks a variant of the COX enzyme that is different from the known variant COX-1 and COX-2. Literature review reveals that simultaneous determination of PCM and TH content in tablets can be achieved by spectrophotometry, [3][4][5][6][7][8][9] high performance thin layer chromatography [10][11][12][13] and reverse phase high performance liquid chromatography [14][15][16][17][18]. Among the different analytical equipments and techniques, Reverse Phase High Performance Liquid Chromatography (RP-HPLC) stands out due to its resolution, specificity, accuracy, precision and cost effectiveness.…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC method for estimation of tramadol hydrochloride and paracetamol in pharmaceutical formulation

Hemant¹,

Krishna²,

Varaprasad³

et al. 2019

GSC Biol. and Pharm. Sci.

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A simple, rapid and sensitive RP-HPLC method was developed for the quantitative determination of tramadol hydrochloride and paracetamol in combined tablet dosage form. The chromatographic analysis was carried out on enable C18G column (250 x 4.6 mm, 5 μm) with mobile phase containing 1 % glacial acetic acid: acetonitrile (50:50 v/v). The flow rate of mobile phase was 1.0 mL/min and effluents were monitored at 272 nm. The retention times of tramadol hydrochloride and paracetamol were 2.032 min and 2.711 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. The method was found to simple, rapid and sensitive and was successfully applied to the estimation of tramadol hydrochloride and paracetamol in combined dosage form.

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“…HPSAM transforms the incorrigible error to a constant systematic error, which can then be estimated and corrected. Previous studies highlight the high versatility and applicability of the method [26][27][28][29][30][31][32][33][34]. In this study, a detailed attempt has been made to simultaneously determine BHA and BHT using HPSAM in a simple, sensitive, and highly selective manner.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

Özgür

Kalaycıoğlu

Dülger

2017

Maced. J. Chem. Chem. Eng.

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A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point standard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spectra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4–20 μgml–1(r = 0.9981) for BHA and 20–100 μgml–1(r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml–1for BHA and 0.72 and 2.41 μgml–1for BHT, respectively. The percentage recovery ranges were 100.44–102.50 % for BHA and 96.45–100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values (< 2 %). The results obtained using HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.

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Simultaneous Spectrophotometric Determination of Tramadol and Acetaminophen in Pharmaceutical Formulations Using H-Point Standard Addition Method

Cited by 3 publications

References 19 publications

The Chemometric Evaluation of the Factors Influencing Cloud Point Extraction for Fluoroquinolones

The Chemometric Evaluation of the Factors Influencing Cloud Point Extraction for Fluoroquinolones

RP-HPLC method for estimation of tramadol hydrochloride and paracetamol in pharmaceutical formulation

Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method

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