Single Cell Analysis by Using ICP-MS

Miyashita, Shin-ichi; Fujii, Shin-ichiro; Shigeta, Kaori; Inagaki, Kenji

doi:10.1007/978-4-431-56463-8_5

Cited by 9 publications

(12 citation statements)

References 56 publications

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“…High sampling resolution and quasi-simultaneous detection of a wide range of m/z values accomplished with ICP-ToF instrumentation are the key features. The price to be paid is a worse sensitivity in comparison to quadrupole or high resolution ICP instrumentation [49]. In fact, relevance and potential of this strategy has resulted in the development of specialized ICP-ToF instruments for this application [50], and instrumental upgrades and developments are being carried out in order to provide more robust and sensitive technology [48,49].…”

Section: Icp-ms As Immunoassays Detector: Size (Nano) Mattersmentioning

confidence: 99%

A reflection on the role of ICP-MS in proteomics: Update and future perspective

Calderón-Celis

Encinar

2019

Journal of Proteomics

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Mass spectrometry is the technique of reference for the identification and quantification of proteins. Whereas ESI and MALDI ionization sources are inherently not quantitative being highly influenced by the chemical nature of the analyte and the matrix, ICP-MS uses a hard ionization source that destroys proteins into its atoms and measures the elemental signal, which is independent of its chemical environment. As a consequence, ICP-MS turns up as an excellent technique for the screening, mapping and quantification of peptides and proteins in a sample through elemental detection (any element but C, H, N, or O) once they have been previously separated by chromatography. In this time, great efforts have been put in developing instrumentation and new methodologies that enable a better, more efficient, and more useful analysis of proteins with ICP-MS. Moreover, quantitative capabilities but lack of molecular information of ICP has led to a synergic relationship both with identifying capabilities of ESI-MS, or the use of protein-specific antibodies carrying an elemental label. Journal SignificanceWe are delighted to participate in this special issue and have the chance to congratulate Journal of Proteomics for its 10 th Anniversary, and wish for many further successful anniversaries. During this last decade, Journal of Proteomics has been a clear promotor of works integrating ICP-MS for proteomics analysis. In fact, already in 2009, a review was published by invitation of the editor in chief focused on the established and potential role of ICP-MS in different areas of the proteomics analysis at the time: "The emerging role of ICP-MS in proteomics" [1]. Even though ICP-MS is not fully known or acknowledged in the proteomics world yet, its impact was significant as demonstrated by the really high interest in such publication (over 150 citations). Since then, several excellent papers relating to ICP-MS applications in proteomics have been published in this journal.Following the trend, we expect through this personal view of the current standing of ICP-MS in proteomics to enlighten the readers of Journal of Proteomics with a vision of the full present and future potential of ICP-MS in proteomics.

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Section: Icp-ms As Immunoassays Detector: Size (Nano) Mattersmentioning

confidence: 99%

A reflection on the role of ICP-MS in proteomics: Update and future perspective

Calderón-Celis

Encinar

2019

Journal of Proteomics

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“…Among the synchrotron-based techniques XANES is even able to discriminate between different elemental species [ 37 ] ( Table 1 A) and can thus potentially discriminate between intact NPs internalized from the external environment versus NPs precipitated in situ from internalized Ag + ions. The emerging method of single cell SP-ICP-MS [ 38 ] may in the future provide a bridge between quantification and localization, and single cell LA-ICP-MS [ 39 ] is already making strides in this direction although its spatial resolution (~2.5 μm) is not sufficient to identify individual NPs.…”

Section: Analyses Of Analytical Techniques For Quantification Of Nmentioning

confidence: 99%

The Biological Fate of Silver Nanoparticles from a Methodological Perspective

et al. 2018

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We analyzed the performance and throughput of currently available analytical techniques for quantifying body burden and cell internalization/distribution of silver nanoparticles (Ag NPs). Our review of Ag NP biological fate data shows that most of the evidence gathered for Ag NPs body burden actually points to total Ag and not only Ag NPs. On the other hand, Ag NPs were found inside the cells and tissues of some organisms, but comprehensive explanation of the mechanism(s) of NP entry and/or in situ formation is usually lacking. In many cases, the methods used to detect NPs inside the cells could not discriminate between ions and particles. There is currently no single technique that would discriminate between the metals species, and at the same time enable localization and quantification of NPs down to the cellular level. This paper serves as an orientation towards selection of the appropriate method for studying the fate of Ag NPs in line with their properties and the specific question to be addressed in the study. Guidance is given for method selection for quantification of NP uptake, biodistribution, precise tissue and cell localization, bioaccumulation, food chain transfer and modeling studies regarding the optimum combination of methods and key factors to consider.

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“…In order to fully understand a biological system such as a cell, it is necessary to determine the trace metals within the system, due to their essential roles in biological or physiological functions. In contrast with stochastic average analysis by bulk measurements, the quantitative analysis of individual cells leads to a more sensitive representation of cell-to-cell variations of content of elements (Miyashita et al 2017). In this context, single-cell elemental analysis is considered one of the major research fields in metallomics in the next decade (Haraguchi 2017a).…”

Section: Detection and Quantitative Distribution Of Metals And Metal Nanoparticles At The Single-cell Levelmentioning

confidence: 99%

“…In recent years, time-resolved ICP-MS, known as single-cell ICP-MS (SC-ICP-MS), has attracted a great deal of interest for multielemental analysis of single cells as an alternative of classical bulk analysis of large amounts of cells with a lysis, extraction, or digestion (Miyashita et al 2014a(Miyashita et al , 2017Mueller et al 2014;Wang et al 2017a). This technique is able to detect and quantify metallic elements in individual cells with high sensitivity, with the advantage of obtaining information about cellto-cell variations instead of the average information from large cell populations obtained by conventional bulk measurements.…”

Section: Time Resolved Icp-ms Analysismentioning

confidence: 99%

“…Briefly, a cell suspension is introduced into the plasma (via conventional concentric nebulizers or other dedicated introductions systems as it will be discussed later) where droplets containing cells are vaporized and the intrinsic metals inside the cell are atomized and ionized, producing an ion plume that is detected by the ICP-MS as a short transient signal or a spike signal (also called cell event). Each individual cell produces its own ion plume with a typical duration in the range of 100-500 μs (Miyashita et al 2017). If the acquisition time (i.e., dwell time) of the detector is longer than the duration of the ion plume generated, signals corresponding to individual cells can be detected as high spikes, whose signal is proportional to the amount of metals in the cell.…”

Section: Time Resolved Icp-ms Analysismentioning

confidence: 99%

See 1 more Smart Citation

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

Jiménez-Lamana

Szpunar

Łobiński

2018

Advances in Experimental Medicine and Biology

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Single cells represent the basic building units of life, and thus their study is one the most important areas of research. However, classical analysis of biological cells eludes the investigation of cell-to-cell differences to obtain information about the intracellular distribution since it only provides information by averaging over a huge number of cells. For this reason, chemical analysis of single cells is an expanding area of research nowadays. In this context, metallomics research is going down to the single-cell level, where high-resolution high-sensitive analytical techniques are required. In this chapter, we present the latest developments and applications in the fields of single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS), mass cytometry, laser ablation (LA)-ICP-MS, nanoscale secondary ion mass spectrometry (nanoSIMS), and synchrotron X-ray fluorescence microscopy (SXRF) for single-cell analysis. Moreover, the capabilities and limitations of the current analytical techniques to unravel single-cell metabolomics as well as future perspectives in this field will be discussed.

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Single Cell Analysis by Using ICP-MS

Cited by 9 publications

References 56 publications

A reflection on the role of ICP-MS in proteomics: Update and future perspective

A reflection on the role of ICP-MS in proteomics: Update and future perspective

The Biological Fate of Silver Nanoparticles from a Methodological Perspective

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

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