Single-cell Protein and Xylitol Production by a Novel Yeast Strain Candida intermedia FL023 from Lignocellulosic Hydrolysates and Xylose

Abstract

Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol production by a novel yeast strain, Candida intermedia FL023, from lignocellulosic hydrolysates and xylose. This strain efficiently assimilated hexose, pentose, and cellubiose for cell mass production with the crude protein content of 484.2 g kg−1 dry cell mass. SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0.86 g L−1 h−1 and the yield of 0.40 g g−1 sugar. SCP was also produced using NaOH-pretreated Miscanthus sinensis straw and corn steep liquor as the carbon and nitrogen sources through simultaneous saccharification and fermentation with the dry cell productivity of 0.23 g L−1 h−1 and yield of 0.17 g g−1 straw. C. intermedia FL023 was tolerant to 0.5 g L−1 furfural, acetic acid, and syringaldehyde in xylitol fermentation and produced 45.7 g L−1 xylitol from xylose with the productivity of 0.38 g L−1 h−1 and the yield of 0.57 g g−1 xylose. This study provides feasible methods for feed and food additive production from the abundant lignocellulosic bioresources.

Genomic and transcriptomic analysis of Candida intermedia reveals the genetic determinants for its xylose-converting capacity

“…b, c Reciprocal read mapping, where b displays the CBS 141442 genome reads coloured according to which chromosome they belong to in the CBS 141442 assembly, and mapped to the PYCC 4715 chromosomes, and c marked reads from PYCC 4715 mapped to the CBS 141442 assembly. *Breakpoints confirmed with PCR previously for C. intermedia PYCC 4715 and FL023 [20,43]. The specific growth rate was only slightly affected in 200 g L −1 of glucose (0.33 h −1 ) ( Fig.…”

Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass

“…Both W. anomalus and B. adeninivorans grew faster and achieved a much higher biomass concentration after 24 h, in particular B. adeninivorans which reached a CDW of 44.0 g/L (as compared to 29.7 g/L and 18.0 g/L for W. anomalus and C. jadinii, respectively). This is in the same range as fed-batch cultures of C. intermedia growing on corncob hydrolysates which reached a CDW of 34.6 g/L after 40 h of incubation [26]. In another study, C. jadinii grown on rice polishing in batch reached a CDW of 50 g/L after 65 h [27].…”

“…C. jadinii and W. anomalus used most of the glucose, showed good biomass productivity, amounting to 2.51 and 2.61 g/L/h, respectively. This is clearly higher than productivities of 1.15 and 0.86 g/L/h achieved for other candida strains produced on rice polishing [27] and corncob hydrolysates [26], respectively. For continuous cultures of C. jadinii using molasses as carbon source, a maximum productivity of 2.15 g/L/h has been reported [31].…”

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

“…In recent years, yeasts isolated from various natural habitats have been found to havepotential as effective producers of various biotechnological products worldwide. Various wild yeast species have been reported to have a vital role in modern industrial biotechnology, for example, for the production of citric acid [51], single cell proteins [52,53], ethanol [54,55], microbial oils [56][57][58], indole-3-acetic acid (IAA) [59], itaconic acid [60], glycolipid biosurfactants [49], arabitol [61], xylitol [62], erythritol and mannitol [63]. In this study, we isolated 217 yeast strains of 10 species in the Ascomycota and 35 species in the Basidiomycota.…”

“…Some of these species have been reported to have industrial biotechnological potential. Candida intermedia, Candida tropicalis and Pichia kudriavzevii have been reported to produce ethanol and single-cell protein [52][53][54][55] whereas Wickerhamomyces anomalus has been found to have the ability to produce ethanol and xylitol [54,62]. Meyerozyma caribbica has exhibited potential for xylitol production [64].…”