Single ether group in a glycol-based ultra-thin layer prevents surface fouling from undiluted serum

Abstract

Through systematic structural modification, it is shown that the internal, single oxygen atom of simple monoethylene glycol-based organic films is essential for radically altering the fouling behaviour of quartz against undiluted serum, as characterized by the electromagnetic piezoelectric acoustic sensor. The synergy is strongest with distal hydroxyls.

A true theranostic approach to medicine: Towards tandem sensor detection and removal of endotoxin in blood

“…4. In comparison, plasma adsorption on bare quartz caused a much greater shift in resonant frequency of Δf $ À28 kHz, close to the value recorded for full serum (Δf ¼ À30.9 73.7 kHz) (Sheikh et al, 2012). Of higher importance for LPS biosensing is the entirely different specific response profile obtained with plasma samples spiked with high dose of LPS (1000 pg/mL), an endotoxemia level of the order of magnitude that can be found in patients in dire need of treatment (Opal et al, 1999) such as those admitted to intensive care units (Marshall et al, 2002).…”

“…SI-4). With respect to hemocompatibility, the presence of MEG-OH background areas -formed during PMB attachment (vide supra) -is certainly beneficial since this type of coating has been shown to display remarkable antithrombogenicity (Fedorov et al, 2014), in addition to pronounced antifouling properties (Sheikh et al, 2012) ( Fig. SI-3).…”

“…(Note: in the process, longer (dark grey) MEG-TFA diluent residues are converted into shorter (light grey) MEG-OH chains (Sheikh et al, 2012;Fedorov et al, 2014). Bottom/right panel: chemical structures of OEG-TTTA cross-linker, MEG-TFA diluent, PMB receptor probe and LPS target analyte.…”

“…As shown schematically in Fig. 2, PMB immobilization in an aqueous medium results in a surface chemistry, wherein OEG-TTTA cross-linking residues are PMB-derivatized while, upon cleavage of their labile trifluoroacetyl terminal moiety (Sheikh et al, 2012;Fedorov et al, 2014), MEG-TFA diluent residues are converted into shorter MEG-OH chains ║-OSi(OH) 2 -(CH 2 ) 3 -O-(CH 2 ) 2 -OH that all together produce/provide an antifouling background (Sheikh et al, 2012).…”

Sacrificial BSA to block non‐specific adsorption on organosilane adlayers in ultra‐high frequency acoustic wave sensing

“…Sensing relies on the acoustic wave physics of our electromagnetic piezoelectric acoustic sensor (EMPAS), a highly sensitive flow-injection device able to perform measurements in a real-time and label-free manner at tunable frequency. [14,[18][19][20][21][22][23] Biosensing properties are evaluated and discussed in terms of avidin specific and non-specific adsorption (NSA). Sacrificial bovine serum albumin (BSA) is used to block NSA.…”