Single-step ELISA capillary sensor based on surface-bonded glucose oxidase, antibody, and physically-adsorbed PEG membrane containing peroxidase-labeled antibody

Sensitive “capillary ELISA” via vapor-phase surface modification

“…6(b)). Previously a square capillary ELISA reported LOD of less than 1.0 ng/mL for IgG [18]. However, the capillary tube was processed by a complex surface treatment to equip chemically linked biomolecules and physically adsorbed peroxidase in polyethyelene glycol membrane.…”

“…Also in the POC applications, various versions of capillary tube-based immunoassay systems were developed taking advantage of its simple structural feature [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Rectangular-shaped capillary tubes were used to perform enzyme immunoassay for various types of biomarkers [18,[22][23][24]. When the well-known surface chemistry based on 3-aminoprolytriethoxysilane (APTES) and glutaraldehyde (GA) were used to install antibody in the capillary tube, the immunoassay results produced 10 ng/mL level detection capability [24].…”

“…When the well-known surface chemistry based on 3-aminoprolytriethoxysilane (APTES) and glutaraldehyde (GA) were used to install antibody in the capillary tube, the immunoassay results produced 10 ng/mL level detection capability [24]. When the inner surface of the rectangular glass was equipped with multilayered functional coatings, high degree of detectability was able to be produced with limit of detection (LOD) of 0.1 ng/mL [18]. However this method required complex steps to prepare multi-layered functional coatings.…”

Fabrication and packaging of a mass-producible capillary-assembled microchip for simple and multiplexed bioassay

“…We have developed various glass capillary biosensors to be integrated into CAs-CHIP, to measure various analytes such as proteins [25][26][27][28], ions [29,30], enzymes [30,31], and metabolites [32,33] Among many capillary biosensors developed, dry reagentrelease capillary (dRRC) which allows spontaneous dissolution of analytical reagents and selective reaction to give fluorescence response by sample introduction via capillary action, played an important role for the simplicity in capillary biosensor preparation and biosensing [30]. However, both the first (CAs-CHIP-1G) [23,30,31] and second (CAs-CHIIP-2G) [35] generations of CAs-CHIP requires the meticulous one-by-one capillary embedding onto a PDMS microchannel.…”

Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary-Isoelectric Focusing with Rhodamine 110-based Substrates

“…[12][13][14][15][16][17][18][19][20][21][22][23][24][25] Especially, for enzyme activity assays, 18-23 the inner surface of a capillary is covered with a soluble coating containing reagents for the enzyme activity assay. Introducing a sample solution containing enzymes into the RRCs by a capillary action allows for the spontaneous release of the reagents and subsequent mixing with the solution.…”