Single‐Use Bioreactors – An Overview

Jossen, Valentin; Eibl, Regine; Eibl, Dieter

doi:10.1002/9781119477891.ch4

Cited by 24 publications

(24 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of single‐use bioreactors [79–81] for the production of PCTC‐based food ingredients is only beneficial for research and development due to size limitations and the high prices of the pharma‐grade plastic vessels or bags. Due to their homogeneous energy dissipation, wave‐mixed bioreactors [82], such as the BIOSTAT RM (Sartorius), the Wave Bioreactor (Cytiva, formerly GE Healthcare), the HyPerforma Rocker Bioreactor (ThermoFisher) or the CELL‐tainer (Celltainer Biotech) are often used.…”

Section: Commercial Food Ingredients Based On Plant Cell Culture Techmentioning

confidence: 99%

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

Gubser

Vollenweider

Eibl

et al. 2021

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Climate change and an increasing world population means traditional farming methods may not be able to meet the anticipated growth in food demands. Therefore, alternative agricultural strategies should be considered. Here, plant cell and tissue cultures (PCTCs) may present a possible solution, as they allow for controlled, closed and sustainable manufacturing of extracts which have been or are still being used as colorants or health food ingredients today. In this review we would like to highlight developments and the latest trends concerning commercial PCTC extracts and their use as food ingredients or even as food. The commercialization of PCTC‐derived products, however, requires not only regulatory approval, but also outstanding product properties or/and a high product titer. If these challenges can be met, PCTCs will become increasingly important for the food sector in coming years.

show abstract

Section: Commercial Food Ingredients Based On Plant Cell Culture Techmentioning

confidence: 99%

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

Gubser

Vollenweider

Eibl

et al. 2021

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…The growing acceptance of single-use bioreactor systems made of plastics, which are increasingly used as alternatives (Simaõ et al 2016) The vulnerability of bacteria, yeast and algae depends on the production process (e.g., biofuel production processes have a relatively low vulnerability in comparison to biopharmaceutical production processes). For reasons of clarity, the literature sources have been summarized below the table to gold standard stainless steel systems, especially in the biopharmaceutical industry (Eibl et al 2018;Jossen et al 2019;Werner et al 2019), can be explained by the technical requirements and durations of cell culture processes. In mammalian cell culture processes, a good hygienic design concept and the avoidance of potential (cross-)contamination are essential, which can be achieved more easily by using single-use bioreactors.…”

Section: Reusable Vs Single-usementioning

confidence: 99%

“…The growing acceptance of single-use bioreactor systems made of plastics, which are increasingly used as alternatives to gold standard stainless steel systems, especially in the biopharmaceutical industry (Eibl et al 2018 ; Jossen et al 2019 ; Werner et al 2019 ), can be explained by the technical requirements and durations of cell culture processes. In mammalian cell culture processes, a good hygienic design concept and the avoidance of potential (cross-)contamination are essential, which can be achieved more easily by using single-use bioreactors.…”

Section: Reusable Vs Single-usementioning

confidence: 99%

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Schirmer

Maschke

Pörtner

et al. 2021

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

No matter the scale, stirred tank bioreactors are the most commonly used systems in biotechnological production processes. Single-use and reusable systems are supplied by several manufacturers. The type, size, and number of impellers used in these systems have a significant influence on the characteristics and designs of bioreactors. Depending on the desired application, classic shaft-driven systems, bearing-mounted drives, or stirring elements that levitate freely in the vessel may be employed. In systems with drive shafts, process hygiene requirements also affect the type of seal used. For sensitive processes with high hygienic requirements, magnetic-driven stirring systems, which have been the focus of much research in recent years, are recommended. This review provides the reader with an overview of the most common agitation and seal types implemented in stirred bioreactor systems, highlights their advantages and disadvantages, and explains their possible fields of application. Special attention is paid to the development of magnetically driven agitators, which are widely used in reusable systems and are also becoming more and more important in their single-use counterparts.Key Points• Basic design of the most frequently used bioreactor type: the stirred tank bioreactor• Differences in most common seal types in stirred systems and fields of application• Comprehensive overview of commercially available bioreactor seal types• Increased use of magnetically driven agitation systems in single-use bioreactors

show abstract

“…The distinctive feature of SUBs is the use of disposable bag-like containers, as vessels made from multi-layer plastics [1]. Many different SUBs are currently available on the market, with rocking bioreactors being the most popular [2]. The liquid phase inside a bag-like container is agitated by the oscillatory movement of the bioreactor platformthis motion results in wave propagation on the liquid surface.…”

Section: Introductionmentioning

confidence: 99%

“…shear-sensitive mammalian cells cultures and integrated forms of biomass, e.g. hairy roots, and bubble-free aeration without sparging equipment [2,3].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of the sensor method for the determination of mixing time in a rocking single-use bioreactor

2021

Chemical Technology and Engineering. Proceedings

View full text Add to dashboard Cite

The aim of the study was to determine the characteristics of mixing time in a rocking singleuse bioreactor equipped with a 2-litre bag-like container by the sensor method. Results have shown that the frequency of rocking movement has the biggest influence on the values of mixing time.Кеуwordssingle-use bioreactor, wave-type agitation, rocking motion, mixing time, sensor method, design of experiments.

show abstract

Single‐Use Bioreactors – An Overview

Cited by 24 publications

References 64 publications

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Adaptation of the sensor method for the determination of mixing time in a rocking single-use bioreactor

Contact Info

Product

Resources

About