Single-Walled Carbon Nanohorn (SWNH) Aggregates Inhibited Proliferation of Human Liver Cell Lines and Promoted Apoptosis, Especially for Hepatoma Cell Lines

Zhang, Jinqian; Huang, Rui

doi:10.5772/61109

Cited by 1 publication

(1 citation statement)

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“…There are already reports in the literature about the toxicity of pristine SWCNHs during tests both in vitro [ 6 , 7 , 8 ], on different cell lines, and in vivo during respiratory, oral, and skin or eye sensitization [ 9 , 10 ]. It is also proven that SWCNHs are getting inside cells [ 7 , 11 , 12 , 13 ], but no cancerogenic/mutual activity was observed [ 9 ]. However, after modification, the toxicity can change and it is hard to predict the direction of change.…”

Section: Introductionmentioning

confidence: 99%

Nitric-Acid Oxidized Single-Walled Carbon Nanohorns as a Potential Material for Bio-Applications—Toxicity and Hemocompatibility Studies

et al. 2021

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The results of in vitro studies of single-walled carbon nanohorn (SWCNH) oxidized materials’ cytotoxicity obtained by the cell membrane integrity (Neutral Red Uptake (NRU)) and metabolic activity (by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)) on A549 and human dermal fibroblasts (HDF) cell lines are presented. We also present hemocompatibility studies on human and porcine blood, and an erythrocyte concentrate to prove that the obtained samples will not interfere with blood components. Characterization of the materials is supplemented by ζ-potential measurements, Transmission Electron Microscope (TEM) imaging, and thermogravimetric studies (TG). The presented results show the correlation between the specific surface area of materials and the platelet aggregation, when the ID/IG ratio determined from Raman spectra correlates with hemoglobin release from the erythrocytes (in whole blood testing). A plausible mechanism explaining the observed correlations is given. The cytotoxicity and hemocompatibility studies prove that the studied materials are acceptable for use in biomedical applications, especially a sample SWCNH-ox-1.5 with the best application potential.

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