Single-walled carbon nanotube, multi-walled carbon nanotube and Fe<sub>2</sub>O<sub>3</sub> nanoparticles induced mitochondria mediated apoptosis in melanoma cells

Naserzadeh, Parvaneh; Esfeh, Fatemeh Ansari; Kaviani, Mahboubeh; Ashtari, Khadijeh; Kheirbakhsh, Raheleh; Salimi, Ahmad; Pourahmad, Jalal

doi:10.1080/15569527.2017.1363227

Cited by 30 publications

(18 citation statements)

References 35 publications

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“…Exposure to SWCNT and MWCNT caused severe OS (102), inflammation, and histopathological alterations in various organs and cells, including the lungs (103), kidneys (15), liver (104), human skin HaCat cells (105), melanoma cells (106), lung cells (107), human microvascular endothelial cells (108), human embryonic kidney (HEK293) cells (15), human neuroblastoma cells (109), human mesothelial cells (110), and human keratinocytes (42). The increased contents of reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS), severe disruption in biomembranes, and disturbed mitochondrial redox state are well-described events involved in the mechanisms of CNT-mediated cytotoxicity (14,105,108,111).…”

Section: Discussionmentioning

confidence: 99%

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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“…Since the cytotoxic effects of ALA-PDT depend on targeting of mitochondria [39], in this study we aimed to specifically evaluate mitochondrion-affecting parameters including MMP and ROS generation [40]. A change in trans-membrane potential of the mitochondrion is one of the earliest changes occurs during apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Akbarzadeh

Khoshgard

Arkan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Selective accumulation of photosensitizers (PSs) into cancerous cells is one of the most important factors affecting photodynamic therapy (PDT) efficacy. 5-Aminolevulinic acid (5-ALA) is precursor of a strong PS, protoporphyrin-IX (Pp-IX); but it has poor permeability in lipophilic membrane of the cells due to its hydrophilic property. Therefore, establishment of an improved delivery strategy could highly affect on treatment outcome. Moreover, folate receptors (FRs) are overexpressed on the surface of many tumor cells. In the present work, targeting ligand folic acid (FA) and 5-ALA conjugated bismuth oxide nanoparticles (FA-5ALA-Bi 2 O 3 NPs) were synthesized; and used in PDT against human nasopharyngeal carcinoma cells (KB cell line). The KB cells incubated with the synthesized NPs for 2 h; then illuminated using a custom-made red light LED lamp at the light dose of 26 J/cm 2. MTT and caspase-3 activity assays were performed to evaluate the efficacy of treatment. Results showed that FR targeting ligand enables selective endocytosis of FA-5-ALA-conjugated NPs into KB cells. Improved internalization of 5-ALA into cells decreased the cell viability to about 50%, 65%, and 85% in the groups receiving FA-5ALA-Bi 2 O 3 NPs, 5ALA-Bi 2 O 3 NPs, free 5-ALA and subsequent PDT, respectively. Therefore, FA-5ALA-Bi 2 O 3 NPs can significantly increase the cell killing effect of PDT.

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“…Nevertheless, some deviation from this model should be considered. Indeed, different reports have shown that in several experimental models, different stimuli that increase intracellular ROS elicit markers of intrinsic apoptotic pathways that can be inhibited by CsA, including mitochondrial proapoptotic protein release, phosphatidylserine exposure, and DNA fragmentation [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48]. This suggests that, at least under selected experimental conditions, submaximal MPT might represent an alternative path for apoptosis activation.…”

Section: Involvement Of Mpt In Rcd Subroutinesmentioning

confidence: 99%

Physiopathology of the Permeability Transition Pore: Molecular Mechanisms in Human Pathology

et al. 2020

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Mitochondrial permeability transition (MPT) is the sudden loss in the permeability of the inner mitochondrial membrane (IMM) to low-molecular-weight solutes. Due to osmotic forces, MPT is paralleled by a massive influx of water into the mitochondrial matrix, eventually leading to the structural collapse of the organelle. Thus, MPT can initiate outer-mitochondrial-membrane permeabilization (MOMP), promoting the activation of the apoptotic caspase cascade and caspase-independent cell-death mechanisms. The induction of MPT is mostly dependent on mitochondrial reactive oxygen species (ROS) and Ca2+, but is also dependent on the metabolic stage of the affected cell and signaling events. Therefore, since its discovery in the late 1970s, the role of MPT in human pathology has been heavily investigated. Here, we summarize the most significant findings corroborating a role for MPT in the etiology of a spectrum of human diseases, including diseases characterized by acute or chronic loss of adult cells and those characterized by neoplastic initiation.

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Single-walled carbon nanotube, multi-walled carbon nanotube and Fe₂O₃ nanoparticles induced mitochondria mediated apoptosis in melanoma cells

Abstract: Our promising results provide a potential approach for the future therapeutic use of SWCNT, MWCNT, and IONPs in melanoma through mitochondrial targeting.

Cited by 30 publications

References 35 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Physiopathology of the Permeability Transition Pore: Molecular Mechanisms in Human Pathology

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Single-walled carbon nanotube, multi-walled carbon nanotube and Fe2O3 nanoparticles induced mitochondria mediated apoptosis in melanoma cells

Abstract: Our promising results provide a potential approach for the future therapeutic use of SWCNT, MWCNT, and IONPs in melanoma through mitochondrial targeting.

Cited by 30 publications

References 35 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Physiopathology of the Permeability Transition Pore: Molecular Mechanisms in Human Pathology

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Single-walled carbon nanotube, multi-walled carbon nanotube and Fe₂O₃ nanoparticles induced mitochondria mediated apoptosis in melanoma cells