Sister chromatid exchanges and structural chromosome aberrations in relation to age and sex

Hedner, Karin; Högstedt, Benkt; Kolnig, A.-M.; Mark-Vendel, E.; Strömbeck, Bodil; Mitelman, Felix

doi:10.1007/bf00304544

Cited by 80 publications

(18 citation statements)

References 27 publications

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“…Literature and personal data suggest an increase in spontaneous chromosome abnormalities frequency in healthy controls with age. 27,28 Therefore, for the statistical analysis we consider two groups of control subjects. In the first group (age 5 -49), the frequency of cells with aberrations varies between 0 and 9.1% with a mean value of 5.4% and a standard deviation (SD) of 2.9, while in the second group (age 60 -90), the frequency of cells with abnormalities ranges between 4 and 14% with a mean value of 9.2% and an SD of 3.5.…”

Section: Resultsmentioning

confidence: 99%

“…It has been demonstrated that in normal subjects, the level of induced apoptosis depends on the age of the donors 30 and that the frequency of chromosome alterations in peripheral blood lymphocytes increases with age. 27,28 A decrement with the age in the ability of programmed death for cells with chromosome abnormalities could explain both the high frequency of chromosome rearrangements present in healthy donors over 60 and the higher risk to develop a cancer with increasing age. Chromosome instability in NBS patients' parents and relatives has been rarely investigated: some authors report no chromosome abnormalities, 31 -33 while others describe the presence of chromosome rearrangements.…”

Section: Discussionmentioning

confidence: 99%

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Chromosome instability and nibrin protein variants in NBS heterozygotes

et al. 2003

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The frequency of spontaneous chromosome abnormalities in peripheral blood lymphocytes and the X-ray G2 sensitivity in lymphoblastoid cell lines (LCL) have been evaluated in heterozygous subjects from three unrelated Nijmegen Breakage Syndrome (NBS) families, characterised by different mutations in the NBS1 gene. In all the 13 NBS heterozygotes analysed, we found spontaneous chromosome instability consisting in chromosome and chromatid breakages and rearrangements, while radiosensitivity was similar to that of control LCLs in seven out of eight tested NBS heterozygotes. The densitometric analysis of nibrin by immunoblotting indicated only a slight reduction in some of the LCLs from NBS carriers, whereas the immunoprecipitation assay appears a more reliable tool to detect NBS carriers. By means of immunoprecipitation, we investigated two homozygous and four heterozygous subjects. In the cells of the NBS patient 668, with the mutation 900del25, an alternative form of nibrin with a molecular weight of approximately 55 kDa has been detected. This variant protein, together with the normal p95, was also found in the LCL 34 established from a carrier of the same family. Signals of nibrin with a molecular weight lower than 95 kDa, but higher than that observed in LCLs 668 and 34, were detected also in three carriers from the family with mutation 835del4.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chromosome instability and nibrin protein variants in NBS heterozygotes

et al. 2003

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“…Esses achados estão de acordo com os observados por vários autores (Bender et al, 1988;Galloway et al, 1986;Hedner et al, 1982;Margolin & Shelby, 1985), enquanto que outros autores não encontraram nenhum efeito com relação ao sexo (Anderson et al, 1991).…”

Section: Discussionunclassified

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Pacheco

Hackel

2002

Cad. Saúde Pública

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“…Previous studies [10,14] have reported age as a significant factor affecting chromosome aberrations. Our results indicate that not only age but also sex may be involved; i.e.…”

Section: Discussionmentioning

confidence: 99%

Chromosome Aberrations in Lymphocytes of Residents Living in Buildings Constructed with Radioactively Contaminated Rebars

Wang-Wuu¹,

Tai

Wuu

et al. 2001

Journal of Biomedical Science

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Using the G-banding technique, we examined lymphocytes from 90 individuals (43 males and 47 females, median age 31 years) living in buildings constructed with radioactively contaminated rebars. Forty-five nonexposed control subjects (22 males and 23 females, median age 30 years), matched to the radiation-exposed individuals by sex and age, were selected for comparison. At least 500 metaphases were checked for each individual. All recognizable structural aberrations of chromosomes or chromatids were recorded. After adjusting for age and smoking status, both the percentage of cells with aberrant chromosomes (PCAC) and the number of aberrant chromosomes per 100 cells (NAC) were found to be significantly higher in the radiation-exposed females than in the control females (p < 0.05 for PCAC and NAC). This difference, however, was not observed in the comparison of radiation-exposed and control males. This suggests a possible interaction between sex and radiation exposure in their effects on chromosome aberrations.

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Sister chromatid exchanges and structural chromosome aberrations in relation to age and sex

Cited by 80 publications

References 27 publications

Chromosome instability and nibrin protein variants in NBS heterozygotes

Chromosome instability and nibrin protein variants in NBS heterozygotes

Instabilidade cromossômica induzida por agroquímicos em trabalhadores rurais na região de Passo Fundo, Rio Grande do Sul, Brasil

Chromosome Aberrations in Lymphocytes of Residents Living in Buildings Constructed with Radioactively Contaminated Rebars

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