The automated assessment and analysis of employee activity in a manufacturing enterprise, operating in accordance with the concept of Industry 4.0, is essential for a quick and precise diagnosis of work quality, especially in the process of training a new employee. In the case of industrial solutions, many approaches involving the recognition and detection of work activity are based on Convolutional Neural Networks (CNNs). Despite the wide use of CNNs, it is difficult to find solutions supporting the automated checking of work activities performed by trained employees. We propose a novel framework for the automatic generation of workplace instructions and real-time recognition of worker activities. The proposed method integrates CNN, CNN Support Vector Machine (SVM), CNN Region-Based CNN (Yolov3 Tiny) for recognizing and checking the completed work tasks. First, video recordings of the work process are analyzed and reference video frames corresponding to work activity stages are determined. Next, work-related features and objects are determined using CNN with SVM (achieving 94% accuracy) and Yolov3 Tiny network based on the characteristics of the reference frames. Additionally, matching matrix between the reference frames and the test frames using mean absolute error (MAE) as a measure of errors between paired observations was built. Finally, the practical usefulness of the proposed approach by applying the method for supporting the automatic training of new employees and checking the correctness of their work done on solid fuel boiler equipment in a manufacturing company was demonstrated. The developed information system can be integrated with other Industry 4.0 technologies introduced within an enterprise.