Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets

Mu, Qingxin; Su, Gaoxing; Liu, Liwen; Gilbertson, Ben O.; Yu, Lam H.; Zhang, Qiu; Sun, Ya‐Ping; Yan, Bing

doi:10.1021/am300253c

Cited by 341 publications

(331 citation statements)

References 33 publications

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“…GO has smoother edges and hydrophilic properties, and seems to be less potent in terms of penetrating cell compartments and interacting with DNA, resulting in the absence of genotoxic effects. Furthermore, it has been demonstrated that GO particles smaller than 500 nm may be internalized via clathrin-mediated 34 On the other hand, Yue et al 35 showed that 350 nm GO are wrapped by active filopodia of macrophages, while 2 µm GO enter cells nearly perpendicularly. This mechanism may trap GO inside vesicles, making it impossible for GO to penetrate into the nucleus and interact directly with DNA.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Hinzmann¹,

Jaworski²,

Kutwin³

et al. 2014

IJN

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The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised of pristine graphene, reduced graphene oxide, graphene oxide, graphite, and ultradispersed detonation diamond in a U87 cell line. The scope of the work consisted of structural analysis of the nanoparticles using transmission electron microscopy, evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 μg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.

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Section: Discussionmentioning

confidence: 99%

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Hinzmann¹,

Jaworski²,

Kutwin³

et al. 2014

IJN

View full text Add to dashboard Cite

show abstract

“…However, the underlying mechanisms of cellular internalization of GBMs remain enigmatic and several pathways have been proposed. The two main working hypotheses include phagocytosis and clathrin-mediated endocytosis 30,31 but the possibility of membrane translocation through a "piercing effect" has also been revealed from computational studies 32,33 . Remarkably, photothermal effect due to the ability of graphene to absorb NIR light was suggested to enhance the transfection efficiency thanks to induced heating which locally disrupts the organization of the lipid bilayer cell membrane, hence rendering it more permeable and facilitating endosomal escape 34 .…”

Section: What Can Gbms Offer As Gene Delivery Platforms?mentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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“…Almost no inhibition of cell proliferation was found at doses up to 100 µg/mL. 166 The in vitro hemocompatibility and genotoxicity of GO with human primary blood components remains a hotly …”

Section: Cytotoxicitymentioning

confidence: 99%

Current applications of graphene oxide in nanomedicine

Wu¹,

An²,

Hulme³

2015

IJN

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Graphene has attracted the attention of the entire scientific community due to its unique mechanical and electrochemical, electronic, biomaterial, and chemical properties. The water-soluble derivative of graphene, graphene oxide, is highly prized and continues to be intensely investigated by scientists around the world. This review seeks to provide an overview of the currents applications of graphene oxide in nanomedicine, focusing on delivery systems, tissue engineering, cancer therapies, imaging, and cytotoxicity, together with a short discussion on the difficulties and the trends for future research regarding this amazing material.

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Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets

Cited by 341 publications

References 33 publications

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Graphene materials as 2D non-viral gene transfer vector platforms

Current applications of graphene oxide in nanomedicine

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