Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells

Li, Yang; Sun, Lei; Jin, Minghua; Du, Zhongjun; Liu, Xiaomei; Guo, Caixia; Li, Yanbo; Huang, Peili; Sun, Zilong

doi:10.1016/j.tiv.2011.05.003

Cited by 171 publications

(115 citation statements)

References 39 publications

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“…1,2 The size and surface area of materials are crucial characteristics from a toxicological perspective. [3][4][5] Because nanomaterials have an increased surface area compared with bulk materials, nanomaterials are expected to be more reactive and have more interaction with living organisms.…”

Section: Introductionmentioning

confidence: 99%

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Yoo¹,

Yoon²,

Kwon³

et al. 2012

IJN

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Background: Titanium dioxide (TiO 2 ) has been widely used in many areas, including biomedicine, cosmetics, and environmental engineering. Recently, it has become evident that some TiO 2 particles have a considerable cytotoxic effect in normal human cells. However, the molecular basis for the cytotoxicity of TiO 2 has yet to be defined. Methods and results:In this study, we demonstrated that combined treatment with TiO 2 nanoparticles sized less than 100 nm and ultraviolet A irradiation induces apoptotic cell death through reactive oxygen species-dependent upregulation of Fas and conformational activation of Bax in normal human cells. Treatment with P25 TiO 2 nanoparticles with a hydrodynamic size distribution centered around 70 nm ) together with ultraviolet A irradiation-induced caspase-dependent apoptotic cell death, accompanied by transcriptional upregulation of the death receptor, Fas, and conformational activation of Bax. In line with these results, knockdown of either Fas or Bax with specific siRNA significantly inhibited TiO 2 -induced apoptotic cell death. Moreover, inhibition of reactive oxygen species with an antioxidant, N-acetyl-L-cysteine, clearly suppressed upregulation of Fas, conformational activation of Bax, and subsequent apoptotic cell death in response to combination treatment using TiO 2 P25-70 and ultraviolet A irradiation. Conclusion:These results indicate that sub-100 nm sized TiO 2 treatment under ultraviolet A irradiation induces apoptotic cell death through reactive oxygen species-mediated upregulation of the death receptor, Fas, and activation of the preapoptotic protein, Bax. Elucidating the molecular mechanisms by which nanosized particles induce activation of cell death signaling pathways would be critical for the development of prevention strategies to minimize the cytotoxicity of nanomaterials.

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Section: Introductionmentioning

confidence: 99%

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Yoo¹,

Yoon²,

Kwon³

et al. 2012

IJN

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show abstract

“…The cytotoxicity of 1.4 nm Au particles was sixfold higher than the cytotoxicity of 0.8, 1.2, and 1.8 nm [15]. Studying Ag nanoparticles of 10, 80, and 113 nm, Park et al [71] found in increase in cytotoxicity linked to a decrease in size, whereas Li et al [72] reported an increase in cellular toxicity when the particle size of amorphous silica nanoparticle decreased from 68 to 19 nm.…”

Section: Determinants Of Human Inhalation Hazards Of Persistent Enginmentioning

confidence: 97%

Human health hazards of persistent inorganic and carbon nanoparticles

Reijnders

2012

J Mater Sci

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Persistent inorganic and carbon nanoparticles are increasingly engineered for applications and may also be present in conventional materials such as carbon black. Furthermore, they may originate from conventional non particulate materials by processes such as wear and tear. Persistent inorganic and carbon nanoparticles can be hazardous to humans. Relatively much research regards the hazards of inhaled nanoparticles. These may give rise to respiratory disease and to negative effects on other organs, including the cardiovascular system. Determinants of risk of inhaled nanoparticles include: number, size, surface characteristics, shape, structure, and the formation of assemblages. These determinants should preferentially be considered in exposure metrics. A major molecular mechanism underlying the inhalation hazard of nanoparticles is the generation of reactive oxygen species, but other mechanisms such as interactions with proteins and DNA may also contribute. Health hazards may also be linked to ingestion of persistent inorganic and carbon nanoparticles, dermal exposure and exposure of the eye. Standards for workplace exposure to persistent inorganic and carbon are currently emerging and there are options for hazard reduction by elimination and substitution of hazardous nanoparticles and by engineering controls.

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“…However, in the CCK-8 assay, which contains WST-8, viable cells can convert WST-8 to a watersoluble formazan, thus avoiding the solubilization step and enabling the simplification of assay performance. 55 In our preset study, both the MTT and CCK-8 assay were used and combined in order to measure the cytotoxicity of nanoporous silica against Caco-2 cells. The results of MTT and CCK-8 assays in Figure 10 indicate that both 3DOM silica and SBA-15 silica exhibited negligible cytotoxicity on Caco-2 cells at tested concentrations.…”

Section: In Vitro Cell Cytotoxicity Evaluationsmentioning

confidence: 99%

Ordered nanoporous silica as carriers for improved delivery of water insoluble drugs: a comparative study between three dimensional and two dimensional macroporous silica

Wang¹,

Zhao²,

Hu³

et al. 2013

IJN

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Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells

Cited by 171 publications

References 39 publications

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation

Human health hazards of persistent inorganic and carbon nanoparticles

Ordered nanoporous silica as carriers for improved delivery of water insoluble drugs: a comparative study between three dimensional and two dimensional macroporous silica

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