Skeletal muscle histochemistry in male and female Andalusian and Arabian horses of different ages

Rivero, José‐Luis L.; Galisteo, A. M.; Agüera, Estrella I.; Miró, F.

doi:10.1016/0034-5288(93)90051-g

Cited by 51 publications

(30 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar sex-related difference has also been documented for skeletal muscles. 22,23 The significant correlations between body weight and LV mass, LVIDd, and MWT, with larger horses having larger hearts, were consistent with previous findings. 6,7 Although valvular regurgitation increased in prevalence during the study period (up to 87% for the horses that were racing), no increase in severity of regurgitation was observed.…”

Section: Discussionsupporting

confidence: 90%

Echocardiographic evaluation of changes in left ventricular size and valvular regurgitation associated with physical training during and after maturity in Standardbred trotters

Buhl¹,

Ersbøll²

2012

javma

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Echocardiographic evaluation of changes in left ventricular size and valvular regurgitation associated with physical training during and after maturity in Standardbred trotters

Buhl¹,

Ersbøll²

2012

javma

View full text Add to dashboard Cite

show abstract

“…After birth, the percentage of Type-I fibers increased in several skeletal muscles of pig and dog, which is likely the consequence of a pronounced changeover from developmental to adult MHC isoforms (Suzuki and Cassens, 1980;Lefaucheur et al, 1995;Š trbenc et al, 2006). Late-age effect on the transformation of adult MHC isoforms was described in different horse breeds by Roneus et al (1991) and Rivero et al (1993) using histochemistry. They established that the percentage of MHC-I and -IIa fibers increased and that of MHCIIx decreased in horse gluteus medius muscle up to the age of 6 years.…”

Section: Discussionmentioning

confidence: 94%

Enzyme‐ and immunohistochemical aspects of skeletal muscle fibers in brown bear (Ursus arctos)

Smerdu¹,

Cehovin

Štrbenc

et al. 2008

Journal of Morphology

View full text Add to dashboard Cite

To further elucidate the pattern of MHC isoform expression in skeletal muscles of large mammals, in this study the skeletal muscles of brown bear, one of the largest mammalian predators with an extraordinary locomotor capacity, were analyzed. Fiber types in longissimus dorsi, triceps brachii caput longum, and rectus femoris muscles were determined according to the myofibrillar ATPase (mATPase) histochemistry and MHC isoform expression, revealed by a set of antibodies specific to MHC isoforms. The oxidative (SDH) and glycolytic enzyme (alpha-GPDH) capacity of fibers was demonstrated as well. By mATPase histochemistry five fiber types, i.e., I, IIC, IIA, IIAX, IIX were distinguished. Analyzing the MHC isoform expression, we assume that MHC-I, -IIa, and -IIx are expressed in the muscles of adolescent bears. MHC-I isoform was expressed in Type-I fibers and coexpressed with presumably -IIa isoform, in Type-IIC fibers. Surprisingly, two antibodies specific to rat MHC-IIa stained those fast fibers, that were histochemically and immunohistochemically classified as Type IIX. This assumption was additionally confirmed by complete absence of fiber staining with antibody specific to rat MHC-IIb and all fast fiber staining with antibody that according to our experience recognizes MHC-IIa and -IIx of rat. Furthermore, quite high-oxidative capacity of all fast fiber types and their weak glycolytic capacity also imply for MHC-IIa and -IIx isoform expression in fast fibers of bear. However, in adult, full-grown animal, only MHC-I and MHC-IIa isoforms were expressed. The expression of only two fast isoforms in bear, like in many other large mammals (humans, cat, dog, goat, cattle, and horse) obviously meets the weight-bearing and locomotor demands of these mammals.

show abstract

“…The muscle fibre types in horses change with age and spontaneous activity from birth until " 6 y (Rivero et al 1993). The relative frequency of mATPase IIA fibres in the gluteus muscle of Andalusian horses increases over the first 3 y of age and that of IIX high-oxidative fibres decreases after this age.…”

Section: mentioning

confidence: 99%

Distribution of fast myosin heavy chain‐based muscle fibres in the gluteus medius of untrained horses: mismatch between antigenic and ATPase determinants

1999

View full text Add to dashboard Cite

The distribution of muscle fibres classified on the basis of their content of different myosin heavy chain (MHC) isoforms was analysed in muscle biopsies from the gluteus medius of adult untrained horses by correlating immunohistochemistry with specific anti-MHC monoclonal antibodies and standard myofibrillar ATPase (mATPase) histochemistry. Percutaneous needle biopsies were taken at 3 depths (20, 40 and 60 mm) from 4 4-y-old Andalusian stallions. The percentage of ' pure ' I MHC fibres increased whereas that for pure IIX MHC fibres decreased from the most superficial to the deepest sampling site. Within the fast fibres, types IIA and IIAX MHC-classified fibres were proportionately more abundant in the deepest sampling site than in the superficial region of the muscle. The immunohistochemical and histochemical characterisation of a large number of single fibres (n l 1375) was compared and correlated on a fibre-to-fibre basis. The results showed that 40 % of the fibres analysed were pure type I (expressing only MHC-I) ; they showed correct matching between their antigenic and mATPase determinants. In contrast, within the fast fibres, a considerable proportion of fibres were found showing a mismatch between their immunohistochemical and mATPase profiles. The most common mismatched fibre phenotypes comprised fibres displaying coexpression of both fast MHCs when analysed by immunocytochemistry, but showing an mATPase profile similar to typical IIX fibres (moderate mATPase reaction after preincubation at pH 4.4). Considered altogether, the total mismatched fibres represented only 4.2 % of the whole fast fibre population in the superficial region of the muscle, but their proportion increased to 15.6 % and 38.4 % in the middle and deep regions, respectively, of gluteus medius. It is concluded that a considerable number of hybrid fast MHC IIAX fibres are present in the gluteus medius of untrained horses, suggesting that equine type II fibres have probably been misclassified in numerous previous publications based on the use of histochemistry alone. This has important implications in attempts to study the physiological properties of fast fibre types adequately in horses.

show abstract

Skeletal muscle histochemistry in male and female Andalusian and Arabian horses of different ages

Cited by 51 publications

References 26 publications

Echocardiographic evaluation of changes in left ventricular size and valvular regurgitation associated with physical training during and after maturity in Standardbred trotters

Echocardiographic evaluation of changes in left ventricular size and valvular regurgitation associated with physical training during and after maturity in Standardbred trotters

Enzyme‐ and immunohistochemical aspects of skeletal muscle fibers in brown bear (Ursus arctos)

Distribution of fast myosin heavy chain‐based muscle fibres in the gluteus medius of untrained horses: mismatch between antigenic and ATPase determinants

Contact Info

Product

Resources

About