Skeletal muscles respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days

Abstract

Results of this study indicate that a TSAA deficiency affects muscle properties in a muscle-dependent manner increasing the oxidative capacity of RM and reducing growth and glycolytic metabolism of LM.

Splanchnic tissues respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days

“…We reported previously that the relative weight, protein mass and protein synthesis of skeletal muscles were, respectively, 11, 17 and 47 % lower in pigs receiving a TSAA-deficient diet [19]. Using the same animals, no change in the weights of the splanchnic tissues was observed in the present study, which agrees with observations indicating that a greater priority for nutrient utilization is given to (or taken by) the intestines than to other tissues [5,17].…”

“…The supply of TSAA affected performance with lower weight gain in pigs receiving TSAA− diet than those receiving TSAA+ diets (292 vs. 375 g/day, respectively; P < 0.01; [19]). …”

“…The experimental procedures have been described in detail in a study on the effect of TSAA supply on the composition and metabolism of skeletal muscles [19]. The same piglets were used in both studies.…”

“…The concentration of the reduced form of glutathione (GSH) was calculated by subtracting 2 × GSSG from the total glutathione concentration. Tissues were analyzed in duplicate for dry matter, crude protein, and AA concentrations [19]. Whole-tissue AA contents included both protein-bound and free AA and were expressed as g/16 g of N (which corresponds to 100 g tissue protein).…”

“…Tissues were analyzed for the fractional rate and efficiency of protein synthesis (amount of protein synthesized per milligram of RNA), RNA/protein ratio (an indicator of the cellular potential for protein synthesis), activities of proteasome and calpain, and glutathione concentrations in the reduced (GSH) and oxidized (GSSG) forms according to the procedures described previously [19]. Briefly, the fractional rate of tissue protein synthesis (percentage of protein mass synthesized per day) was determined using the flooding dose procedure [10,21] using the following equation:…”