Skeletal regeneration for segmental bone loss: Vascularised grafts, analogues and surrogates

Dalisson, Benjamin; Charbonnier, Baptiste; Aoude, Ahmed; Gilardino, Mirko S.; Harvey, Edward J.; Makhoul, Nicholas; Barralet, Jake E.

doi:10.1016/j.actbio.2021.09.053

Cited by 40 publications

(26 citation statements)

References 285 publications

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“…However, when the length of a bone defect reaches 1.5 times of the diameter of the shaft, it exceeds the critical size of autologous repair, thereby resulting in bone resorption and nonunion 12,13 . Therefore, how to use tissue engineering technology to repair bone tissue defects becomes the focus of surgeons 14,15 . Tissue engineering technology mainly includes seed cells, scaffold materials, and other stimulating factors.…”

Section: Introductionmentioning

confidence: 99%

“… 12 , 13 Therefore, how to use tissue engineering technology to repair bone tissue defects becomes the focus of surgeons. 14 , 15 Tissue engineering technology mainly includes seed cells, scaffold materials, and other stimulating factors. MSCs, an important member of the stem cell family, are mainly derived from the early mesoderm and a small part of the ectoderm.…”

Section: Introductionmentioning

confidence: 99%

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MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

et al. 2022

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Objectives: Delivery systems that provide time and space control have a good application prospect in tissue regeneration applications, as they can effectively improve the process of wound healing and tissue repair. In our experiments, we constructed a novel micro-RNA delivery system by linking framework nucleic acid nanomaterials to micro-RNAs to promote osteogenic differentiation of mesenchymal stem cells. Materials and Methods:To verify the successful preparation of tFNAs-miR-26a, the size of tFNAs-miR-26a were observed by non-denaturing polyacrylamide gel electrophoresis and dynamic light scattering techniques. The expression of osteogenic differentiation-related genes and proteins was investigated by confocal microscope, PCR and western blot to detect the impact of tFNAs-miR-26a on ADSCs. And finally, Wnt/β-catenin signaling pathway related proteins and genes were detected by confocal microscope, PCR and western blot to study the relevant mechanism.Results: By adding this novel complex, the osteogenic differentiation ability of mesenchymal stem cells was significantly improved, and the expression of alkaline phosphatase (ALP) on the surface of the cell membrane and the formation of calcium nodules in mesenchymal stem cells were significantly increased on days 7 and 14 of induction of osteogenic differentiation, respectively. Gene and protein expression levels of ALP (an early marker associated with osteogenic differentiation), RUNX2 (a metaphase marker), and OPN (a late marker) were significantly increased. We also studied the relevant mechanism of action and found that the novel nucleic acid complex promoted osteogenic differentiation of mesenchymal stem cells by activating the canonical Wnt signaling pathway.Conclusions: This study may provide a new research direction for the application of novel nucleic acid nanomaterials in bone tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

et al. 2022

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“…Several alternatives to autologous bone grafting have emerged, such as allografts, xenografts, and synthetic grafts. These alternatives are available in various forms and in large quantities, but each has specific indications and limitations, and mixed results have been obtained 5,6 . Therefore, there is an urgent need for the development of a new generation of bone substitutes.…”

Section: Introductionmentioning

confidence: 99%

“…These alternatives are available in various forms and in large quantities, but each has specific indications and limitations, and mixed results have been obtained. 5,6 Therefore, there is an urgent need for the development of a new generation of bone substitutes. Tissue-engineered bone substitutes consisting of cells, biomaterials, and bioactive molecules have become a viable approach for bone defect reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Engineering hypertrophic cartilage grafts from lipoaspirate for critical‐sized calvarial bone defect reconstruction: An adipose tissue‐based developmental engineering approach

Huang

Yan

et al. 2022

Bioengineering & Transla Med

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Developmental engineering of living implants from different cell sources capable of stimulating bone regeneration by recapitulating endochondral ossification (ECO) is a promising strategy for large bone defect reconstruction. However, the clinical translation of these cell-based approaches is hampered by complex manufacturing procedures, poor cell differentiation potential, and limited predictive in vivo performance.We developed an adipose tissue-based developmental engineering approach to overcome these hurdles using hypertrophic cartilaginous (HyC) constructs engineered from lipoaspirate to repair large bone defects. The engineered HyC constructs were implanted into 4-mm calvarial defects in nude rats and compared with decellularized bone matrix (DBM) grafts. The DBM grafts induced neo-bone formation via the recruitment of host cells, while the HyC pellets supported bone regeneration via ECO, as evidenced by the presence of remaining cartilage analog and human NuMApositive cells within the newly formed bone. However, the HyC pellets clearly showed superior regenerative capacity compared with that of the DBM grafts, yielding more new bone formation, higher blood vessel density, and better integration with adjacent native bone. We speculate that this effect arises from vascular endothelial growth factor and bone morphogenetic protein-2 secretion and mineral deposition in the HyC pellets before implantation, promoting increased vascularization and bone formation upon implantation. The results of this study demonstrate that adipose-derived HyC constructs can effectively heal large bone defects and present a translatable therapeutic option for bone defect repair.

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“…Bone defects resulting from serious injury, trauma, or medical surgery 1,2 have become one of the most prevalent clinical issues. Bone defects have limited scale self-healing capability in most cases, but critical-sized bone defects require surgery interventions.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia inducible factor-1 signaling pathway in macrophage involved angiogenesis in materials-instructed osteo-induction

et al. 2022

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Skeletal regeneration for segmental bone loss: Vascularised grafts, analogues and surrogates

Cited by 40 publications

References 285 publications

MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

Engineering hypertrophic cartilage grafts from lipoaspirate for critical‐sized calvarial bone defect reconstruction: An adipose tissue‐based developmental engineering approach

Hypoxia inducible factor-1 signaling pathway in macrophage involved angiogenesis in materials-instructed osteo-induction

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