Skin cancer

Craythorne, Emma; Al-Niami, Firas

doi:10.1016/j.mpmed.2017.04.003

Cited by 45 publications

(36 citation statements)

References 3 publications

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“…37 The application of LFU, regardless of the application mode, resulted in a greater penetration of ZnPc in the dermis (Table 5). As non-melanoma and melanoma tumors are originally sited in the epidermis layer; 60,61 higher penetration of ZnPc up to the basal layer of the epidermis is the minimum requirement to secure drug uptake by the target cells and increase sonodynamic activation of the drug for the treatment of skin cancer. Therefore, for in vitro penetration studies, the greater amount of drug accumulated in deeper layers of the skin (ie in the dermis), the better evidence that the drug was capable of spreading throughout the viable epidermis and reaching tumor cells at high concentrations.…”

Section: Dovepressmentioning

confidence: 99%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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Purpose: Sonodynamic therapy (SDT) is a new therapeutic modality for the noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Topical SDT requires the development of delivery systems to properly transport the sonosensitizer, such as zinc phthalocyanine (ZnPc), to the skin. In addition, the delivery system itself can participate in sonodynamic events and influence the therapeutic response. This study aimed to develop ZnPc-loaded micelle to evaluate its potential as a topical delivery system and as a cavitational agent for low-frequency ultrasound (LFU) application with the dual purpose of promoting ZnPc skin penetration and generating reactive oxygen species (ROS) for SDT. Methods: ZnPc-loaded micelles were developed by the thin-film hydration method and optimized using the Quality by Design approach. Micelles' influence on LFU-induced cavitation activity was measured by potassium iodide dosimeter and aluminum foil pits experiments. In vitro skin penetration of ZnPc was assessed after pretreatment of the skin with LFU and simultaneous LFU treatment using ZnPc-loaded micelles as coupling media followed by 6 h of passive permeation of ZnPc-loaded micelles. The singlet oxygen generation by LFU irradiation of the micelles was evaluated using two different hydrophilic probes. The lipid peroxidation of the skin was estimated using the malondialdehyde assay after skin treatment with simultaneous LFU using ZnPc-loaded micelles. The viability of the B16F10 melanoma cell line was evaluated using resazurin after treatment with different concentrations of ZnPc-loaded micelles irradiated or not with LFU. Results: The micelles increased the solubility of ZnPc and augmented the LFU-induced cavitation activity in two times compared to water. After 6 h ZnPc-loaded micelles skin permeation, simultaneous LFU treatment increased the amount of ZnPc in the dermis by more than 40 times, when compared to non-LFU-mediated treatment, and by almost 5 times, when compared to LFU pretreatment protocol. The LFU irradiation of micelles induced the generation of singlet oxygen, and the lipoperoxidation of the skin treated with the simultaneous LFU was enhanced in three times in comparison to the non-LFU-treated skin. A significant reduction in cell viability following treatment with ZnPc-loaded micelles and LFU was observed compared to blank micelles and non-LFU-treated control groups. Conclusion: LFU-irradiated mice can be a potential approach to skin cancer treatment by combining the functions of increasing drug penetration and ROS generation required for SDT.

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Section: Dovepressmentioning

confidence: 99%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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“…Depending on their subtype BCCs are slow‐growing tumors but can be locally aggressive. They rarely metastasize, and the main morbidity arises from ignored or treated lesions that have recurred (Craythorne & al‐Niami, 2017). They usually arise in head and neck area.…”

Section: Introductionmentioning

confidence: 99%

Multimodal imaging of skin lesions by using methylene blue as cancer biomarker

Malik

Andleeb

Ullah

2020

Microscopy Res & Technique

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This study aimed the optical imaging of malignant and normal skin tissues with multimodal wide-field fluorescence polarization imaging (WF) technique, by using methylene blue as fluorescence dye. We present optical imaging of skin tissues by different techniques, including reflectance, fluorescence, and polarization imaging for early detection of skin cancer. We collected the reflectance confocal images at 390 and 500 nm. For wide-field fluorescence images, specimens were stimulated at 640 nm and images were collected between 670 and 710 nm. The correlation of the regarded optical modalities with histopathology (H&E), their potentials, capabilities, and limitations to detect skin lesions are discussed. The advantages of multimodal imaging of skin tissues are analyzed to divulge possibilities for precise tumor boundary detection and their classification for malignant and nonmalignant skin tissues. Prior to imaging, the cells were stained in aqueous MB (a dye approved by FDA).

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“…El riesgo de desarrollar cáncer de piel se debe a la combinación de factores genéticos y ambientales [3]. La causa más común es la exposición prolongada a la luz ultravioleta [4].…”

Section: Introductionunclassified

“…El cáncer de piel se divide en melanoma maligno y cáncer de piel no-melanoma (NMSC por sus siglas en inglés) [3,4,5]. El melanoma representa el 4 % de los casos de cáncer y es responsable directo del 75 % de todas las muertes causadas por cáncer de piel [6].…”

Section: Introductionunclassified

“…Existen diversos parámetros para diagnosticar melanoma. Algunos de estos son: Asimetría, irregularidad en los bordes, variación de color y diámetro, esté ultimo es controversial puesto que existen melanomas que son menores a 6 mm de diámetro [4]. Sin embargo, estudios recientes demuestran que sigue siendo un parámetroútil para diferenciarlos [11].…”

Section: Introductionunclassified

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