Skin regeneration effect of the Glycosaminoglycans from Liparis tessellatus eggs

Lee, Jae‐Joon; Ticar, Bernadeth F.; Rohmah, Zuliyati; Park, Si-Hyang; Kang, Dawon; Kang, Seok-Joong; Choi, Byeong-Dae

doi:10.1016/j.ijbiomac.2017.08.182

Cited by 3 publications

(1 citation statement)

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“…The proliferation rate of cells was measured using the Cell Titer 96 ® Aqueous One Solution Cell Proliferation Assay purchased from Promega Co. (Madison, WI, USA). The absorbance was measured at 490 nm using the Spectramax M2 Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Hair Growth-Promoting Activities of Glycosaminoglycans Extracted from the Tunics of Ascidian (Halocynthia roretzi)

et al. 2022

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Throughout the ages, hair has had psychological and sociological importance in framing the personality and general appearance of an individual. Despite efforts to solve this problem, no groundbreaking measures have been proposed. Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in homeostatic maintenance and regenerative processes of the skin. However, little is known about the role of these molecules in the regulation of the hair follicle cycle. Three fractions (F1, F2 and F3) were obtained after separation and purification of GAGs from ascidian tunics. F1 was observed to contain a small amount of amino sugar while high contents of galactose and N-acetylglucosamine were noted in F2 and F3. 2-acetamido-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose (∆Di-6S) and 2-acetamido-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose (∆Di-4S) were the main disaccharide components. F3 exhibited the highest proliferation activity on human follicle dermal papilla (HFDP) cells. In addition, mixed samples (FFM) of F2 and F3 at different concentrations showed peak activities for five days. After cell culture at a concentration of 10 mg/mL and dihydrotestosterone (DHT), the inhibition effect was higher than that for Minoxidil. Application of 10 mg of FFM to the hair of mice for 28 days resulted in a hair growth effect similar to that of Minoxidil, a positive control.

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Section: Methodsmentioning

confidence: 99%