Skrining Fitokimia dan Antimikroba Ekstrak Daun Kirinyuh terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli

Abstract

This reseach aims to determine the composition of secondary metabolites of kirinyuh leaf extract (Euphatorium ordoratum.L) and antimicrobial properties of the extract against Staphylococcus aureus and Escherichia coli. The study was conducted in three phases: the first phase is manufacture kirinyuh leaf extract (Euphatprium odoratum L) through maceration method using N-Hexane solvent and methanol. In stage II, phytochemical screening, namely the identification of secondary metabolites (alkaloids, flavonoids, terpenoids and steroids) to the crude extract of leaves kirinyuh (Ephatorium odoratum L) in the form of antimicrobial properties of extracts against Staphylococcus aureus and with variations extract concentrations of 0%, 1 %, 5%, 10%, 15%, 20%, and 25% Echerichia coli and incubated at 37°C for 24 hours. The research found that such compounds secondary metbolit alkaloids, flavonoids, steronoid, and saponin, which dominates the secondary metabolites of four that alkaloid. While the screening test of phytochemical N-hexane extracts is not contain metbolit secondary compounds, testing against bacteria possess bioktivitas inhibition at concentrations of 15% with inhibition zone of 1.3 cm on Escherichia coli bacteria and 1.0 cm in bacteria Staphylococcus aureas. This shows kirinyuh leaf extracts have the ability as an anti-microbial.

Bauji Shallot Variety (Allium ascalonicum L.) Growth Respond And Yield After Kirinyuh Plant Methanol Extract (Chromolaena odorata L.) Treatment

“…This is assumed to be due to various bioactive compounds such as alkaloids, flavonoids, terpenoids, steroids, and saponins contained in Kirinyuh extract, which can trigger plant growth and development. Kirinyuh contains a secondary metabolite substance (Munte et al, 2016) and amino acid (Ngozi et al, 2009). Pengkajian et al (2019) state that biostimulants derived from seaweed extracts contain amino acids, cytokinins, auxins, laminarin, fucoidan, alginate, and betaine which stimulate plant metabolism to increase plant growth and yield.…”

“…Kirinyuh plant is a plant that contains secondary metabolites in the form of alkaloids, flavonoids, steroids, terpenoids, and saponins (Munte et al, 2016) containing nutrients (N, P, K, Ca, Mg) (Koutika & Rainey, 2010) and amino acids (Ngozi et al, 2009) The Kirinyuh extract treatment on mustard greens had a significant effect on germination time (Damayanti, 2012). Research using Kirinyuh extract on horticultural plants such as soybeans, beans, and radishes significantly affected plant height, fresh weight, root length, and pods quantity (Damayanti, 2012).…”

BIOSINTESIS NANOPARTIKEL PERAK (AgNPs) DENGAN EKSTRAK DAUN KIRINYUH (Chromolaena odorata) TERIMPREGNASI ZEOLIT DALAM MENGHAMBAT BAKTERI PENYEBAB JERAWAT

“…Saat ini AgNPs banyak disintesis menggunakan metode biologi atau disebut dengan biosintesis green synthesis. Dalam proses tersebut dibutuhkan reduktor berupa ekstrak tumbuhan, jamur, dan bakteri tetapi reduktor yang yang banyak digunakan berupa ekstrak tumbuhan karena memiliki kelebihan seperti lebih cepat bereaksi dan tidak membutuhkan perlakuan tambahan seperti isolasi mikroorganisme sehingga proses sintesis menjadi lebih mudah (Haryani et al, 2016a;Munte & Lubis, 2016). Pada beberapa penelitian yang telah dilakukan AgNPs yang disintesis dengan ekstrak tumbuhan mampu menghambat pertumbuhan bakteri.…”

“…Sumbawa sebagai salah satu wilayah di Indonesia, yang memiliki potensi keragaman tumbuhan, khususnya untuk tumbuhan obat seperti tumbuhan kirinyuh (Chromolaena odorata). Kirinyuh (Cromolaena odorata) memiliki kandungan senyawa metabolit sekunder seperti flavonoid, steroid (Hidayatullah, 2018), tannin, alkaloid, fenol, dan sponin (Munte & Lubis, 2016). Senyawa-senyawa tersebut berperan sebagai pereduksi ion perak menjadi atom perak dan membentuk nanopartikel perak (Rahmayani et al, 2019).…”

“…Senyawa-senyawa tersebut berperan sebagai pereduksi ion perak menjadi atom perak dan membentuk nanopartikel perak (Rahmayani et al, 2019). Pada penelitian lainnya ekstrak daun kirinyuh juga memiliki kemampuan antibakteri dalam menghambat pertumbuhan beberapa bakteri penyebab penyakit infeksi pada kulit seperti Bacillus cereus, Enterococcus faecalis, Streptococcus pyogenes, Proteus vulgaris, termasuk bakteri Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes (Fadia et al, 2020;Munte & Lubis, 2016).…”