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This reseach aims to determine the composition of secondary metabolites of kirinyuh leaf extract (Euphatorium ordoratum.L) and antimicrobial properties of the extract against Staphylococcus aureus and Escherichia coli. The study was conducted in three phases: the first phase is manufacture kirinyuh leaf extract (Euphatprium odoratum L) through maceration method using N-Hexane solvent and methanol. In stage II, phytochemical screening, namely the identification of secondary metabolites (alkaloids, flavonoids, terpenoids and steroids) to the crude extract of  leaves kirinyuh (Ephatorium odoratum L) in the form of antimicrobial properties of extracts against Staphylococcus aureus and with variations extract concentrations of 0%, 1 %, 5%, 10%, 15%, 20%, and 25% Echerichia coli and incubated at 37°C for 24 hours. The research found that such compounds secondary metbolit alkaloids, flavonoids, steronoid, and saponin, which dominates the secondary metabolites of four that alkaloid. While the screening test of phytochemical N-hexane extracts is not contain metbolit secondary compounds, testing against bacteria possess bioktivitas inhibition at concentrations of 15% with inhibition zone of 1.3 cm on Escherichia coli bacteria and 1.0 cm in bacteria Staphylococcus aureas. This shows kirinyuh leaf extracts have the ability as an anti-microbial.

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AbstrakPengaruh kadar asam sulfat pada hidrolisis tandan kosong kelapa sawit (tks) dan waktu fermentasi terhadap kadar bioetanol yang dihasilkan telah dilakukan. Tujuannya penelitian adalah untuk mengetahui kemampuan hidrolisis optimum oleh Saccharomyces cerevisiae untuk menghasilkan bioetanol dan gula. Sebanyak 12,5 g serbuk kelapa sawit bekas digunakan dalam percobaan ini. Bubuk tersebut beberapa kali dihidrolisis dengan asam sulfat dalam beberapa variasi konsentrasi. Produksi gula terdeteksi setiap satu jam dengan menggunakan spektrofotometer. Gula difermentasi oleh Saccharomyces cerevisiae dan diinkubasi selama beberapa hari. Hasil penelitian menunjukkan bahwa konsentrasi asam sulfat 2-4% merupakan konsentrasi oprimum untuk produksi gula 30 g / L. Produksi etanol tertinggi 4,94% terjadi setelah dua hari fermentasi. Kata kunci: asam sulfat, fermentasi, bioethanol, Saccharomyces cerevisiae AbstractThe effect of sulphuric acid fermentation of processed palm oil bunch hydrolysis and on ethanol and sugar production was conducted. The aim was to investigate the hydrolytic optimum required by Saccharomyces cerevisiae for ethanol and sugar production. About 12.5 g powdered palm oil bunch was used in this experiment. The powder was hydrolized several times using sulphuric acid in several concentration. Sugar, product of hydrolysis, was detected every one hour using spectrophotometer. The sugar was fermented by Saccharomyces cerevisiae and incubated for several days. Results showed that 2-4% sulphuric acid was oprimum concentration for 30 g/L sugar production. The highest ethanol production 4.94% occurred after two days fermentation.