Sleep deprivation decreases neuronal excitability and responsiveness in rats both in vivo and ex vivo

Borbély, Sándor; Világi, Ildikó; Haraszti, Zsófia; Szalontai, Örs; Hajnik, Tünde; Tóth, Attila; Détári, László

doi:10.1016/j.brainresbull.2017.12.003

Cited by 19 publications

(16 citation statements)

References 74 publications

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“…Previous studies have proved that long-term decline in sleep quality can lead to an imbalance in the autonomic nervous system, resulting in athletes experiencing symptoms similar to those experienced during overtraining, resulting in a decline in immunity and cognitive function (26) . A single episode of sleep deprivation was found to affect the glutamine content in rats, resulting in a decline of the body's ability to exercise (27) Although coaches and athletes believe that sleep is a part of the recovery process, sleep quality is often not considered in the training plan and competition protocol (28) . Due to the lack of attention on the importance of sleep training, in some cases, sleep time is sacri ced by increasing time for physical training or other activities.…”

Section: Discussionmentioning

confidence: 99%

Relationshp Between Sleep Quality, Mood State, and Performance of Elite Air-Rifle Shooters

Lü

Qiu

2021

Preprint

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Background To evaluate the impact of pre-competition sleep quality on the mood and performance of elite air-rifle shooters. Methods This study included 23 elite air-rifle shooters who participated in an air-rifle shooting-competition from April 2019 to October 2019. Sleep time, sleep efficiency, sleep latency, and wake-up time after sleep onset were monitored using actigraphy. The Pittsburgh sleep quality index and Profile of Mood State were used to assess sleep quality. Competitive State Anxiety Inventory-2 was used to evaluate mood state. Results The average time to fall asleep, sleep time, sleep efficiency, and subjective sleep quality were 20.6 ± 14.9 min, 7.0 ± 0.8 h, 85.9 ± 5.3%, and 5.2 ± 2.2, respectively. Sleep quality decreased as the competition progressed. Pre-competition sleep time in female athletes was significantly higher than that on the competition day (P = 0.05). Pre-competition sleep latency was significantly longer in women than in men (P = 0.021). During training and pre-competition, the tension, fatigue, depression, and emotional disturbance were significantly lower in athletes with good sleep quality than in athletes with poor sleep quality. Athletes with good sleep quality had significantly more energy. The PSQI total score was positively correlated with positive emotion, TMD, cognitive anxiety, and somatic anxiety POMS scores, and negatively correlated with energy and self-confidence scores. Race scores and depression and somatic anxiety scores were negatively correlated. Conclusion Poor sleep quality negatively impacted the mood of athletes; however, sleep indices and competition performance of athletes during competitions were not significantly correlated.

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Section: Discussionmentioning

confidence: 99%

Relationshp Between Sleep Quality, Mood State, and Performance of Elite Air-Rifle Shooters

Lü

Qiu

2021

Preprint

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“…For example, sleep deprivation can lead to a significant change in miRNA profiles in rat adipose tissues and brain, and such a change is independent of the plasma level of corticosterone (35). In addition, sleep in experimental animals can be altered by the cortical or intraventricular injection of miRNAs and specific anti-miRNAs into the brain, as suggested by the slow wave activity on electroencephalographic (EEG) (36). The role of miRNAs in the control of sleep quality may be mediated by their ability to regulate the expression of sleep associated Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Genetic variant rs3750625 in the 3'UTR of ADRA2A affects the sleep quality of patients in the ICU by�promoting miR‑34a binding to ADRA2A

et al. 2020

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Poor sleep is very common in patients in the IcU and hence, sleep quality is considered an important aspect of intensive care; however, the underlying mechanisms of poor sleep in patients in the IcU remain unknown. In this study, we aimed to explore the role of rs3750625, which is located in the 3'UTR of adrenoceptor alpha 2A (AdRA2A), in sleep quality. For this purpose, luciferase assay was conducted to investigate the association between miR-34a and AdRA2A, and the effect of rs3750625 on the binding affinity between miR-34a and AdRA2A was examined. RT-qPcR and western blot analysis were carried out to examine the regulatory association between miR-34a and AdRA2A. The differences in sleep time and efficiency were compared between groups carrying the Ac and cc genotypes of rs3750625, respectively. According to the results from an online search, miR-34a could directly bind to the 3'UTR of AdRA2A, and such binding was confirmed by the observation that miR-34a inhibited the luciferase activity of major or minor AdRA2A 3'UTR in a dose-dependent manner in HcN-1A and U251 cells. In addition, the AdRA2A protein and mRNA levels in the HcN-1A and U251 cells were evidently decreased following transfection with miR-34a precursors. Notably, patients in the Ac group exhibited a similar level of miR-34a mRNA expression compared with patients in the cc group; however, the AdRA2A mRNA and protein levels in the cc group were significantly increased in comparison with those in the AC group. In addition, the sleep time and sleep efficiency in the cc group were much higher than those in the Ac group. Furthermore, the mean arterial pressure (MAP) values in both the Ac and cc groups remained stable from 22:00 to 08:00, and the respiratory rates in both groups were quite similar. However, the heart rate of patients in the cc group was much lower than that of patients in the Ac group. On the whole, the findings of this study suggest that the genetic variant rs3750625 in the 3'UTR of AdRA2A affects the sleep quality of patients in the IcU by promoting the binding of miR-34a to AdRA2A, and hence it may serve as a novel biomarker for the prediction of the sleep quality of patients in the IcU.

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“…[66] To investigate whether DMN and MIL affect action potential, we studied the effect of DMN and MIL on current-evoked spikes in primary cortical neurons. Nine depolarizing current steps (10,20,30,40,50,60,70,80 and 90 pA for 100 ms) were injected into neurons, and we found that the rate of current-evoked firing was dramatically decreased after 50 μM DMN (p < 0.001, n = 7) or 50 μM MIL (p < 0.001, n = 6) application of the control level (Figure 6). Taken together, these experiments provide solid evidence that these two flavonoids can suppress the firing of evoked action potential.…”

Section: Dmn and Mil Suppressed The Current-evoked Firing Of Primary Cortical Neuronsmentioning

confidence: 91%

Flavonoid‐Like Components of Peanut Stem and Leaf Extract Promote Sleep by Decreasing Neuronal Excitability

Guo

Shi

Deng

et al. 2021

Molecular Nutrition Food Res

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Scope: Peanut stem and leaf (PSL), a traditional Chinese medicine, is widely used as a dietary supplement to improve sleep quality; however, the underlying mechanism is unclear. Here, the study aims to determine whether active compounds in PSL extract exert their effects by mediating neuronal excitability. Methods and Results: Aqueous PSL extract (500 mg kg −1 BW) increases the duration of total sleep (TS), slow wave sleep (SWS) and rapid eye movement sleep (REMS) in BALB/c mice after 7 and 14 continuous days of intragastric administration. Two PSL extract components with flavonoid-like structures: 4',7-di-O-methylnaringenin (DMN, 61 µg kg −1 BW) and 2'-O-methylisoliquiritigenin (MIL, 12 µg kg −1 BW), show similar effects on sleep in BALB/c mice. Moreover, incubation with DMN (50 µM) and MIL (50 µM) acutely reduces voltage-gated sodium and potassium currents and suppresses the firing of evoked action potential in mouse cortical neurons, indicating the inhibition on neuronal excitability. Meanwhile, RNA-seq analysis predicts the potential regulation of voltage-gated channels, which is according with the molecular docking simulation that both MIL and DMN can bind to voltage gated sodium channels 1.2 (Na v 1.2). Conclusions: DMN and MIL are the active ingredients of PSL that improve sleep quality, suggesting that PSL promotes sleep by regulating the excitability of neurons.

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Sleep deprivation decreases neuronal excitability and responsiveness in rats both in vivo and ex vivo

Cited by 19 publications

References 74 publications

Relationshp Between Sleep Quality, Mood State, and Performance of Elite Air-Rifle Shooters

Relationshp Between Sleep Quality, Mood State, and Performance of Elite Air-Rifle Shooters

Genetic variant rs3750625 in the 3'UTR of ADRA2A affects the sleep quality of patients in the ICU by�promoting miR‑34a binding to ADRA2A

Flavonoid‐Like Components of Peanut Stem and Leaf Extract Promote Sleep by Decreasing Neuronal Excitability

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