‘Slings’ enable neutrophil rolling at high shear

Sundd, Prithu; Gutierrez, Edgar; Koltsova, Ekaterina K.; Kuwano, Yoshihiro; Fukuda, Satoru; Pospieszalska, Maria K.; Groisman, Alexander; Ley, Klaus

doi:10.1038/nature11248

Cited by 163 publications

(253 citation statements)

References 39 publications

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“…For instance, the stabilization of the rolling (e.g., increase of adhesion numbers or decrease of rolling velocities) at high shear stress was observed only for the experiments using cells (21,23). Those differences may be ascribed to the cellular features, such as the formation of microvilli, slings (26), and cell flattening, which also contribute to stable rolling (27).…”

Section: Discussionmentioning

confidence: 99%

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Suzuki

Ogino

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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The authors note that Fig. 4 and Fig. S4 appeared incorrectly. The corrected figures and their legends appear below. The SI has been corrected online.The authors also note that on page 1 of the supporting information, right column, second full paragraph, lines 5-13, "The starting structure was solvated in a 62 × 129 × 69-Å TIP3 water box (6) with 54 chloride ions to neutralize the system, resulting in 53,872 atoms. The system was minimized for three consecutive 500-conjugate gradient steps, during which the protein was first held fixed; next, only the heavy atoms of the protein were held fixed, and finally, all atoms were allowed to move. After the energy minimizations, the system was heated gradually from 0 K to 310 K over 52 ps, and the temperature was maintained using Langevin dynamics" should instead appear as "The starting structure was solvated in a 68 × 116 × 79-Å TIP3 water box (6) with 53 chloride ions to neutralize the system, resulting in 59,606 atoms. The system was minimized for two consecutive 500-conjugate gradient steps, during which the protein was first held fixed; next, only the heavy atoms of the protein were held fixed. After the energy minimizations, the system was heated gradually from 0 K to 310 K over 9.3 ps, and the temperature was maintained using Langevin dynamics. Subsequently, the system was equilibrated by 4 rounds, first the heavy atoms were held fixed, then only backbone atoms, then only Cα atoms, and finally all atoms were allowed to move." These errors do not affect the conclusions of the article.A B C Fig. 4. Steered molecular dynamics (SMD) simulation of CD44 HABD by pulling force. (A) Snapshots of the HABD-HA8 complex at 0 ns (Left) and 3.5 ns (Right) during the SMD simulation. In the SMD simulation, the Cα carbon of the C-terminal residue Ile173 (gray sphere) was pulled at 10 Å/ns in the indicated direction (arrow), whereas the C2 atom of the N-acetylglucosamine residue (green sphere) was kept fixed. In the snapshot after 3.5 ns in the SMD simulation, the C-terminal mechanosensitive latch was completely separated from the α1 helix. (B) The time course of the hydrogen bond donor-acceptor distances between HA NAG1177 and I100 (red) and E52 and Y166 (blue). (C) Schematic depiction of the mechanosensitive latch in the force-induced conformational change of CD44 HABD.

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Section: Discussionmentioning

confidence: 99%

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Suzuki

Ogino

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…A sample of the images of reviews and original research publications of the 'leukocyte cascade': (a) and (b) from Ley et al (2007), in Nature Reviews Immunology; (c) and (d) from Kolaczkowska and Kubes (2013), also in Nature Reviews Immunology; Snudd et al (2012) in Nature; and (f) from Karino and Goldsmith (1987) in Haemostasis and thrombosis. Parts (a)-(d) are classic immunology-type schematics which divide the stages of the leukocyte recruitment process into steps which attribute causality to particular molecular (receptor-ligand) interactions.…”

Section: Review-type Images Curate the Leukocyte Cascadementioning

confidence: 99%

Curating blood: how students’ and researchers’ drawings bring potential phenomena to light

Hay

Pitchford

2016

International Journal of Science Education

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This paper explores students and researchers drawings of white blood cell recruitment. The data combines interviews with exhibit of review-type academic images and analyses of student modeldrawings. The analysis focuses on the material aspects of bioscientific data-making and we use the literature of concrete bioscience modelling to differentiate the qualities of students model-making choices: novelty versus reproduction; completeness versus simplicity; and the achievement of similarity towards selected model targets. We show that while drawing on already published images, some third-year undergraduates are able to curate novel, and yet plausible causal channels in their graphic representations, implicating new phenomenal potentials as lead researchers do in their review-type academic publications. Our work links the virtues of drawing to learn to the disclosure of potential epistemic things, involving close attention to the contours of non-linguistic stuff and corresponding sensory perception of substance; space; time; shape and size; position; and force. The paper documents the authority and power students may achieve through making knowledge rather than repeating it. We show the ways in which drawing on the images elicited by others helps to develop physical, sensory, and sometimes affective relations towards the real and concrete world of scientific practice. ARTICLE HISTORY

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“…These slings lay down an adhesion substrate at the front of the cell that will eventually become weight-bearing as the cell rolls forward [67]. …”

Section: Purinergic Control Of Vascular Inflammationmentioning

confidence: 99%

“…E-selectin (CD62E) also plays important rolls in rolling, but its expression is induced downstream of EC activation by mechanisms involving NFkBdependent gene transcription [65,66]. Recently, new evidence has distinguished a tethering mechanism by which rolling leukocytes utilize a catch-bond system to crawl along the endothelium [67]. As circulating leukocytes make contact with ECs, they form tethers at the trailing edge that are weight-bearing and consist of P-sel/PSGL-1 bonds.…”

Section: Purinergic Control Of Vascular Inflammationmentioning

confidence: 99%

“…This process has been highly studied and is now known to occur in a step-wise manner beginning with local recruitment, rolling (fast and slow), adhesion and final extravasation into the surrounding tissue (reviewed in [62] tethering mechanism by which rolling leukocytes utilize a catch-bond system to crawl along the endothelium [67]. As circulating leukocytes make contact with ECs, they form tethers at the trailing edge that are weight-bearing and consist of P-sel/PSGL-1 bonds.…”

Section: Purinergic Control Of Vascular Inflammationmentioning

confidence: 99%

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Function and Regulation of Pannexin 1 Channels in the Vascular Endothelium

Lohman¹

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The nucleotide adenosine 5'-triphosphate (ATP) has classically been considered the cell's primary energy currency; however, a novel role for ATP as an extracellular autocrine/paracrine signaling molecule has evolved over the past century. Purinergic signaling is now known to regulate a plethora of physiological and pathophysiological processes in almost every organ system. In the vasculature, ATP and its metabolites elicit dual control over blood vessel tone and tissue perfusion, and purinergic signaling events have been implicated in vascular pathologies including atherosclerosis and inflammation.While the importance of extracellular ATP in the vascular system is well recognized, the mechanism(s) mediating the regulated release of the purine from vascular cells are less well understood and are a key target of current investigation. One such ATP-liberation mechanism has been ascribed to the recently identified pannexin (Panx) channels, namely Panx1. Initially, we characterized the expression and localization profiles of Panx isoforms across the systemic vasculature, identifying predominant representation by the Panx1 isoform in both smooth muscle (SMC) and endothelial cells (EC) comprising the blood vessel wall, with more heterogeneous expression profiles observed in specialized vascular systems including the heart, lung and kidney. In particular, the Panx1 isoform is highly expressed in ECs regardless of blood vessel type or size, while Panx1 expression is limited to SMCs of small arteries and arterioles. Panx1 forms hexameric channels in the plasma membrane of ECs, functioning to release ATP in response to a number of stimuli. However, prolonged Panx1 channel activity is extremely detrimental to cell viability. Here we report a novel negative regulatory mechanism that may govern the activity of Panx1 channels in ECs, by which the bioactive gas nitric oxide (NO) covalently modifies two cysteine (Cys) residues in the channel by a process termed S- reinforce the dual effect of purines on peripheral resistance and blood pressure. Purinergic Control of Vascular InflammationWhile the foundation for purinergic control of vascular functions was initially characterized extensively in the context of vascular reactivity and overall blood flow and pressure regulation, it has become increasingly clear that extracellular ATP also plays important regulatory roles in the vascular inflammatory response. Vascular inflammation can be characterized as acute (physiological) or chronic (pathological) in nature.The acute vascular inflammatory response is an innate process of inflammatory cell homing to infected or damaged tissues resulting from integrated cross-talk between circulating leukocytes and the vascular endothelium, primarily at the level of postcapillary venules [61]. This process has been highly studied and is now known to occur in a step-wise manner beginning with local recruitment, rolling (fast and slow), adhesion and final extravasation into the surrounding tissue (reviewed in [62...

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‘Slings’ enable neutrophil rolling at high shear

Cited by 163 publications

References 39 publications

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Curating blood: how students’ and researchers’ drawings bring potential phenomena to light

Function and Regulation of Pannexin 1 Channels in the Vascular Endothelium

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