Small-angle solution scattering using the mixed-mode pixel array detector

Koerner, Lucas J.; Gillilan, Richard E.; Green, Katherine S.; Wang, Suntao; Grüner, Sol M.

doi:10.1107/s0909049510045607

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…Holton & Frankel (2010) calculated that a micrometre-sized lysozyme crystal should yield a complete cryocooled data set to 2 Å resolution, provided that every diffracted X-ray is detected and there is no competing background scatter. Modern pixel array detectors can detect every X-ray with nearly 100% efficiency (Koerner et al, 2011). Graphene can be used to effectively eliminate external background scatter: although no attempt was made to eliminate air scatter in the path length from the collimator to the main beamstop in this proof-of-principle study, one can readily extrapolate how this may be done.…”

Section: Resultsmentioning

confidence: 99%

Graphene as a protein crystal mounting material to reduce background scatter

et al. 2013

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The overall signal-to-noise ratio per unit dose for X-ray diffraction data from protein crystals can be improved by reducing the mass and density of all material surrounding the crystals. This article demonstrates a path towards the practical ultimate in background reduction by use of atomically thin graphene sheets as a crystal mounting platform for protein crystals. The results show the potential for graphene in protein crystallography and other cases where X-ray scatter from the mounting material must be reduced and specimen dehydration prevented, such as in coherent X-ray diffraction imaging of microscopic objects.

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Section: Resultsmentioning

confidence: 99%

Graphene as a protein crystal mounting material to reduce background scatter

et al. 2013

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“…Note that these focal intensities were limited by a conservative choice of the used attenuators, i.e. maximum countable flux per pixel in the detector, and may be different if an integrating detector with a higher dynamic range is used (Koerner et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Versatility of a hard X-ray Kirkpatrick–Baez focus characterized by ptychography

Giewekemeyer

Osterhoff

Bartels

et al. 2013

J Synchrotron Radiat

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In the past decade Kirkpatrick-Baez (KB) mirrors have been established as powerful focusing systems in hard X-ray microscopy applications. Here a ptychographic characterization of the KB focus in the dedicated nano-imaging setup GINIX (Göttingen Instrument for Nano-Imaging with X-rays) at the P10 coherence beamline of the PETRA III synchrotron at HASLYLAB/DESY, Germany, is reported. More specifically, it is shown how aberrations in the KB beam, caused by imperfections in the height profile of the focusing mirrors, can be eliminated using a pinhole as a spatial filter near the focal plane. A combination of different pinhole sizes and illumination conditions of the KB setup makes the prepared optical setup well suited not only for high-resolution ptychographic coherent X-ray diffractive imaging but also for moderate-resolution/large-field-of-view propagation imaging in the divergent KB beam.

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“…The biological interest is primarily in using these methods to examine nonperiodic biologically-derived materials. Recently, the MMPAD has been demonstrated as a quantum limited detector biomacromolecular solution scattering [32].…”

Section: Mixed-mode Pad (Mmpad)mentioning

confidence: 99%

Development and Applications Of Photosensitive Device Systems To Studies Of Biological And Organic Materials

Grüner¹

2012

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The primary focus of the grant is the development of new x-ray detectors for biological and materials work at synchrotron sources, especially Pixel Array Detectors (PADs), and the training of students via research applications to problems in biophysics and materials science using novel x-ray methods. By any measure, the work of this grant has been extremely successful, resulting in technology that has had worldwide impact and roughly 250 papers, abstracts and reports. Details about these accomplishments have been given in the annual progress reports and the 3-year renewal proposals. This Final Progress Report will, therefore, be restricted to a highlevel overview of the most important accomplishments. These major areas of accomplishment include: (1) Development and application of x-ray Pixel Array Detectors. (2) Development and application of methods of high pressure x-ray crystallography as applied to proteins. (3) Studies on the synthesis and structure of novel mesophase materials derived from block co-polymers. Note that this grant was a logical and seamless continuation of an earlier DOE-BER grant to the P.I. when he was at Princeton University. The grant being reported on below commenced when the P.I. moved to Cornell University in 1997. The earlier grant period also had numerous major accomplishments which will not be described below. However, if a single most significant item had to be chosen from that grant period it would be the development of much of the CCD detector technology that now dominates macromolecular data collection at synchrotron x-ray sources [1, 2]. It is fair to say that a majority fraction of the entries of macromolecular structure in the protein data bank resulted from data collected on CCD detectors, much of the technology of which was accomplished under the prior grant. Other significant accomplishments of the prior grant period, which have continued into the 1997-2011 period, were seminal x-ray studies of biomembranes [3-5], development of technology to study macromolecular systems under high pressure [6], and elucidation of bicontinuous mesophase materials derived from block co-polymers [7]. 1.2 Pixel Array Detectors (PADs) 1.2.1 PAD Development Introduction The need for better x-ray detectors has been repeatedly emphasized by the synchrotron radiation community as necessary to improve utilization of synchrotron radiation (SR) resources [8-12]. Significantly, detectors have consistently been cited as the progress-limiting technology at existing storage ring-based synchrotron sources; detectors will be even more limiting at proposed next generation XFEL and ERL sources unless new technologies are developed. For example, exciting SR experiments that have been proposed in areas such as multiple-frame Laue protein crystallography, dynamics of muscle, liquid crystal and polymer phase transitions and dynamics, crack propagation and materials failure, x-ray speckle diffraction, laser processing of materials, photochemical surface reactions, and chemical kinetics. Even though existing SR so...

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Small-angle solution scattering using the mixed-mode pixel array detector

Cited by 7 publications

References 32 publications

Graphene as a protein crystal mounting material to reduce background scatter

Graphene as a protein crystal mounting material to reduce background scatter

Versatility of a hard X-ray Kirkpatrick–Baez focus characterized by ptychography

Development and Applications Of Photosensitive Device Systems To Studies Of Biological And Organic Materials

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