Snapshotting the transient conformations and tracing the multiple pathways of single peptide folding using a solid-state nanopore

Abstract

A solid-state nanopore based method is described for resolving protein-folding-related problems via snapshotting the folding intermediates and characterizing the kinetics of a single peptide.

Encoding Manipulation of DNA‐Nanoparticle Assembled Nanorobot Using Independently Charged Array Nanopores

“…However, whether this will affect the manipulation effectiveness still remains a mystery. In real experiments, strong binding between streptavidin (gold) and biotin (thiol) [ 41 ] could be used to attach biotinylated DNA to streptavidin‐coated nanoparticles for assembling the multi‐leg nanorobot, the substrate could be fabricated by the existing MEMS and CMOS technology, and the surface charge density of each nanopore could be changed by tuning the gate voltage on the embedded metal electrodes in the membrane. Anyway, the experimental setup of the encoding manipulation system is possible in the near future though some challenges still remain to be solved firstly, which is also the future study researchers may focus on.…”

Nanopore-based technologies beyond DNA sequencing

“…In one example, a protein stopper was introduced to immobilize a biotinylated peptide inside a nanopore, allowing the measurement of multiple conformational transition pathways (Fig. 2e) 83 . In another recent report, a DNA lid was added to one side of a lipid-coated nanopore, and proteins were added to the opposite side (Fig.…”

“…Examples include many aspects of the chemistry of thiols introduced as cysteine side chains 85 . Groups other than thiols can be examined after they have been introduced by site-directed chemical 83 . f, Immobilization of a protein (blue) inside a nanopore (grey) using a DNA origami sphere (red) as a NEOtrap (top left) 84 .…”

Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels

“…Specific recognition in the nanopore/nanochannel analyses can be achieved by surface modification of carefully selected probes with a strong interaction with targets. − The captured targets tailor the properties of the nanopore/nanochannel, mainly including space charge and steric hindrance, and change the ionic signal. Up until now, it has realized the detection of multiple targets, covering ions, , small molecules, , polypeptides, , carbohydrates, nucleic acids, , proteins, − and so on. The critical issue regarding specific recognition is to efficiently convert target characteristics into a detectable ionic signal in view of practical necessity for detecting trace targets in a complex matrix.…”