SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce <i>ACO1</i> expression in apple

Jia, Meiru; Li, Xingliang; Wang, Wei; Li, Tianyu; Dai, Zhengrong; Chen, Yating; Zhang, Kaikai; Zhu, Haocheng; Mao, Wendong; Feng, Qianqian; Liu, Liping; Yan, Jiaqi; Zhong, Silin; Li, Bingbing; Jia, Wensuo

doi:10.1111/nph.18040

Cited by 26 publications

(15 citation statements)

References 73 publications

(155 reference statements)

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“…However, the result was different with Lee et al (2007) and Meng et al (2020). This may be caused by the short expression time of VaAPL1 in tobacco mesophyll cells observed in this study (Jia et al, 2022;Ma et al, 2022) ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH − ; Rodrigo-Moreno et al, 2013). High accumulation of ROS destroys redox homeostasis, leads to cell death, and reduces biomass (Czarnocka and Karpiński, 2018).…”

Section: Discussionmentioning

confidence: 65%

“…However, the result was different with Lee et al (2007) and Meng et al (2020) . This may be caused by the short expression time of VaAPL1 in tobacco mesophyll cells observed in this study ( Jia et al, 2022 ; Ma et al, 2022 ). Our results indicated that overexpression of VaAPL1 resulted in a decrease in REC in VaAPL1 -overexpressing A. thaliana plants and OEs after LT stress ( Figures 2F , 5B ).…”

Section: Discussionmentioning

confidence: 83%

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VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis and Tomato

Liang

Wang

et al. 2022

Front. Plant Sci.

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ADP-glucose pyrophosphorylase (AGPase) is a key rate-limiting enzyme involved in starch synthesis. APL1, an AGPase large subunit, plays an important role in the growth and development of grapes; however, its function in withstanding low temperature (LT) remains elusive. Hence, VaAPL1 was cloned from Vitis amurensis (Zuoshan I), and its function was characterized. The gene was highly expressed in the phloem of V. amurensis during winter dormancy (0, −5, and − 10°C). Phylogenetic relationships demonstrated that VaAPL1 was closely genetic related to SlAPL1 (from Solanum lycopersicum), and clustered into I group. Further, VaAPL1 was ectopically expressed in Arabidopsis thaliana (ecotype Columbia, Col) and tomato (“Micro-Tom” tomato) to characterize its function under LT. Compared with Col, the average survival rate of VaAPL1-overexpressing A. thaliana exceeded 75.47% after freezing treatment. Moreover, reactive oxygen species (ROS) content decreased in VaAPL1-overexpressing A. thaliana and tomato plants under LT stress. The activities of AGPase, and starch contents in VaAPL1-overexpressing A. thaliana were higher than in Col after LT stress. The contents of sucrose and glucose were accumulated in overexpressing plants compared with wild-type at 0 h and 24 h after LT stress. Transcriptome sequencing of overexpressing tomato plants revealed involvement in sugar metabolism and the hormone signal pathway, and Ca2+ signaling pathway-related genes were up-regulated. Hence, these results suggest that overexpression of VaAPL1 not only ensured sufficient starch converting into soluble sugars to maintain cell osmotic potential and provided energy, but also indirectly activated signal pathways involved in LT to enhance plant tolerance.

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Section: Discussionmentioning

confidence: 65%

“…However, the result was different with Lee et al (2007) and Meng et al (2020) . This may be caused by the short expression time of VaAPL1 in tobacco mesophyll cells observed in this study ( Jia et al, 2022 ; Ma et al, 2022 ). Our results indicated that overexpression of VaAPL1 resulted in a decrease in REC in VaAPL1 -overexpressing A. thaliana plants and OEs after LT stress ( Figures 2F , 5B ).…”

Section: Discussionmentioning

confidence: 83%

VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis and Tomato

Liang

Wang

et al. 2022

Front. Plant Sci.

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“…The lines with the highest CmNAC‐NOR expression ( CmNAC‐NOR S,N / cr‐nor #2 and #16, CmNAC‐NOR A,S / cr‐nor #9 and #13) were selected via RT‐qPCR and immunoblot assays (Figure S10B–D). The CmNAC‐NOR S,N / cr‐nor lines showed a typical phenotype associated with high levels of ethylene (Li et al, 2021; Jia et al, 2022), such as dwarfed plants with shorter internodes and more branches, while the CmNAC‐NOR A,S / cr‐nor lines showed no significant difference compared to the WT. Further, CmNAC‐NOR S,N overexpression fully rescued the unripening phenotype of the cr‐nor mutant, while CmNAC‐NOR A,S overexpression only partially rescued the unripening phenotype of the cr‐nor mutant (Figure 5A).…”

Section: Resultsmentioning

confidence: 99%

Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening

Wang

Tian

et al. 2022

JIPB

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The NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has climacteric and nonclimacteric fruit ripening varieties and is an ideal model to study fruit ripening. Two natural CmNAC-NOR variants, the climacteric haplotype CmNAC-NOR S,N and the non-climacteric haplotype CmNAC-NOR A,S , have effects on fruit ripening; however, their regulatory mechanisms have not been elucidated. Here, we report that a natural mutation in the transcriptional activation domain of CmNAC-NOR S,N contributes to climacteric melon fruit ripening. CmNAC-NOR knockout in the climacteric-type melon cultivar "BYJH" completely inhibited fruit ripening, while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant fruits. CmNAC-NOR directly activated carotenoid, ethylene, and abscisic acid biosynthetic genes to promote fruit coloration and ripening. Furthermore, CmNAC-NOR mediated the transcription of the "CmNAC-NOR-CmNAC73-CmCWINV2" module to enhance flesh sweetness. The transcriptional activation activity of the climacteric haplotype CmNAC-NOR S,N on these target genes was significantly higher than that of the nonclimacteric haplotype CmNAC-NOR A,S . Moreover, CmNAC-NOR S,N complementation fully rescued the non-ripening phenotype of the tomato (Solanum lycopersicum) cr-nor mutant, while CmNAC-NOR A,S did not. Our results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.

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“…Ethylene is an important regulator of fruit ripening, and ethylene biosynthesis, ethylene receptors, and ethylene response-related genes all affect fruit ripening ( Sunako et al, 1999 ; Varanasi et al, 2011 ; Tan et al, 2013 ). In apple, MdSnRK2-1 can phosphorylate MdHB1 and MdHB2 to enhance their protein stability and transcriptional activity toward MdACO1 , thereby promoting ethylene synthesis and fruit ripening ( Jia et al, 2022 ). Besides, the TF MdMYC2 is responsive to jasmonate treatment and directly binds to the promoters of MdACS1 , MdACO1 , and MdERF3 , thereby activating the ethylene signaling pathway during apple fruit ripening ( Li et al, 2017 ).…”

Section: Ripening and Storage Quality Of Apple Fruitmentioning

confidence: 99%

Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Malus × domestica)

et al. 2022

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Identifying the genetic variation characteristics of phenotypic traits is important for fruit tree breeding. During the long-term evolution of fruit trees, gene recombination and natural mutation have resulted in a high degree of heterozygosity. Apple (Malus × domestica Borkh.) shows strong ecological adaptability and is widely cultivated, and is among the most economically important fruit crops worldwide. However, the high level of heterozygosity and large genome of apple, in combination with its perennial life history and long juvenile phase, complicate investigation of the genetic basis of fruit quality traits. With continuing augmentation in the apple genomic resources available, in recent years important progress has been achieved in research on the genetic variation of fruit quality traits. This review focuses on summarizing recent genetic studies on apple fruit quality traits, including appearance, flavor, nutritional, ripening, and storage qualities. In addition, we discuss the mapping of quantitative trait loci, screening of molecular markers, and mining of major genes associated with fruit quality traits. The overall aim of this review is to provide valuable insights into the mechanisms of genetic variation and molecular breeding of important fruit quality traits in apple.

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SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple

Cited by 26 publications

References 73 publications

VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis and Tomato

VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis and Tomato

Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening

Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Malus × domestica)

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