Sodium(1+)/D-glucose cotransporter based bilayer lipid membrane sensor for D-glucose

Abstract

A new type of amperometric blosensor for glucose was fabricated using a Na+/D-glucose cotransporter as the signal-transducing sensory element that exploits the D-glucose-triggered Na+ ion current through bilayer lipid membranes (BLMs). The planar BLM was formed by the folding method across a small aperture of a thin Teflon film. The Na+/D-glucose cotransporter, isolated and purified from small intestinal brush border membrane of guinea pigs, was embedded into BLMs through proteoliposomes. The number of the protein molecules thus incorporated in the present sensing membrane was estimated to be ca. 10(7). The sensor response was measured as an ionic current through the BLM arising from cotransported Na+ ion flux under a constant applied potential and was only induced by D-glucose above 10(-9) M, but not by the other monosaccharides except for D-galactose. The effect of applied potentials, Na+ and K+ ion concentrations, and the addition of a competitive inhibitor, phlorizin, were scrutinized to characterize the sensor output. The results were briefly discussed in terms of the potential use of the Na+/D-glucose cotransporter as a sensory element for D-glucose.

A Minisensor for the Rapid Screening of Acesulfame-K, Cyclamate, and Saccharin Based on Surface-Stabilized Bilayer Lipid Membranes

“…No signi®cant interferences were noticed from the presence of these compounds. Sensors using BLM-based devices for the determination of a number of compounds such as glutamic acid [18] and sugars have recently appeared in literature [29]. The fabrication of such a sensor requires the incorporation of a receptor [18] or sugar cotransporter [29] and these compounds are not expected presently to provide an interference.…”

Methods of Analysis for Chemicals that Promote/Disrupt Cellular Signaling

“…[7][8][9][10][11] A membrane-protein-based ion-channel sensor for glutamate and active (uphill) transport sensor for glucose were first reported later in 1990 and 1993, respectively. [12][13][14][15] Coulometric biosensors using glutamate receptor (GluR) ion channel protein as a signal-amplifying sensory element that exploits the glutamate-triggered Na + ion current through bilayer lipid membranes have been fabricated. 13 The formation of stable planar bilayer lipid membranes was achieved by applying the folding method across a small circular aperture bored through a thin polyimide film.…”

“…14 The planar BLM was formed by the folding method across a small aperture of a thin Teflon film. The Na + /Dglucose cotransporter, isolated and purified from small intestinal brush border membranes of gulinea pigs, was embedded into BLMs through proteoliposomes.…”

Recognition of hormones by membrane potential and circular dichroism of immobilized protein membranes

“…Novel types of biosensors have also been devel- [10][11][12][13] auxin-receptor ATprecipitation from 10 wt % dichloroethane in methanol. Bovine serum albumin (BSA; essenPase, 14 antibody, 15 maltose binding protein, 16 and Na / /D-glucose 17 or H / /lactose co-transporter 18 ) tially fatty acid free, 9048-46-8) and g-globulin (fraction 2, 3, 9007-83-4) were purchased from or DNA 19 were utilized as receptor sites. Zhou In our previous study, the shifts in membrane BSA and g-globulin were dissolved in a 1 wt % potential, caused by the injection of D-and Ldichloroethane solution of PMLG, respectively.…”