Soft tissue regeneration using leukocyte-platelet rich fibrin after exeresis of hyperplastic gingival lesions: two case reports

Lauro, Alessandro; Abbate, Deena; Dell’Angelo, B.; Iannaccone, G; Scotto, Fabio Paolo; Sammartino, Gilberto

doi:10.1186/s13256-015-0714-5

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…PRF has gained tremendous momentum for a variety of regenerative procedures dentistry, including for the management of extraction sockets [20][21][22][23], gingival recessions [24][25][26], palatal wound closure [27][28][29], and the regeneration of periodontal defects [30], amongst others [31]. In other medical fields, PRF has also been utilized for the management of hard-to-heal leg ulcers including diabetic foot ulcers, venous and chronic leg ulcers, facial soft tissue defects, laparoscopic cholecystectomy, deep nasolabial folds, superficial rhytids, acne scars, chronic rotator cuff tears, and acute traumatic ear drum perforations [32].…”

Section: Discussionmentioning

confidence: 99%

The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes

Miron

Dham²,

Dham³

et al. 2018

Clin Oral Invest

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Objectives Platelet-rich fibrin (PRF) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Due to the variability in the macroscopic morphology/size of PRF membranes observed between patients, we were interested in studying the effects of patient age, gender, and time between blood draw and the start of centrifugation on the size outcomes of PRF membranes. Despite PRF therapy being increasingly more popular in private practice, to date, no study has investigated the effects of the delay between blood draw and the start of centrifugation in a clinical setting. Materials and methods A total of 60 patients enrolled in this study were divided into 6 groups of 10 patients each, including male and female patients categorized into age groups 21-40, 41-60, and 61-80 years. From each patient, a total of five PRF membranes were fabricated from 10-mL tubes following centrifugation starting after 0, 30, 60, 90, and 120 s. In total, 300 PRF membranes were produced in this study to investigate the effects of patient age, gender, and time on the size outcomes of PRF membranes. Results A longer delay by the clinician before starting centrifugation following blood draw led to a smaller final size of PRF membranes. At 90 s following blood draw, a significant (13%) reduction in PRF membrane size was observed. After 120 s, a significant (23%) reduction was observed. Additionally, female patients had on average 17% larger membranes compared to men (p < 0.05, 300 samples). Lastly, the size outcomes of the PRF membranes was largest in patients aged 61-80, followed by those aged 41-60 and 21-40. However, no statistically significant differences in PRF membrane sizes were reported between age groups. Conclusions The time at which a centrifugation procedure begins following blood draw is critical to optimize the size outcomes of PRF membranes. In general, approximately 15 s is required per tube to harvest 9-10 cc of blood. Therefore, a 60-to 90-s interval between blood draw and the start of centrifugation should be a parameter that is respected by clinicians to avoid significant changes in the macroscopic morphology/size of fabricated PRF membranes. Furthermore, females and older patients produced larger membranes, likely due to lower red blood cell counts derived from their peripheral blood. Clinical relevance The findings from the present study demonstrate that on average, a clinician has approximately 60-90 s between blood draw and the start of the centrifugation cycle to produce standard-sized PRF membranes. Shortly thereafter, a significant reduction in size is observed. Additionally, females and older patients were found to produce larger PRF membranes. Centrifugation protocols may therefore be adapted accordingly.

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Section: Discussionmentioning

confidence: 99%

The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes

Miron

Dham²,

Dham³

et al. 2018

Clin Oral Invest

View full text Add to dashboard Cite

show abstract

“…Over the past decade [10], PRF has gained tremendous momentum having been utilized for a variety of dental and medical procedures. In the dental field, PRF has been utilized for the treatment of extraction sockets [14][15][16][17], gingival recessions [18][19][20], palatal wound closure [21][22][23], regeneration of periodontal defects [24], and hyperplastic gingival tissues [25]. In other medical fields, PRF has been utilized for the successful management of hard-to-heal leg ulcers including diabetic foot ulcers, venous leg ulcers, and chronic leg ulcers [26].…”

Section: Introductionmentioning

confidence: 99%

Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?

et al. 2017

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“…Following centrifugation, a three-dimensional fibrin matrix is produced that may serve as a tissue engineering scaffold for various medical procedures that necessitate either soft-or hard-tissue regeneration [11][12][13]. In dentistry alone, PRF has been utilized for the treatment of extraction sockets [14][15][16][17], gingival recessions [18][19][20], palatal wound closure [21][22][23], the regeneration of periodontal defects [24], and hyperplastic gingival tissues [25]. In other medical fields, PRF has been utilized for the successful management of hard-to-heal leg ulcers, including diabetic foot ulcers, venous leg ulcers, and chronic leg ulcers [26].…”

Section: Introductionmentioning

confidence: 99%

A novel method for harvesting concentrated platelet-rich fibrin (C-PRF) with a 10-fold increase in platelet and leukocyte yields

et al. 2019

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Background and objectives Liquid platelet rich fibrin (PRF; often referred to as injectable PRF) has been utilized as an injectable formulation of PRF that is capable of stimulating tissue regeneration. Our research group recently found that following standard L-PRF protocols (2700 RPM for 12 min), a massive increase in platelets and leukocytes was observed directly within the buffycoat layer directly above the red blood cell layer. The purpose of this study was to develop a novel harvesting technique to isolate liquid PRF directly from this buffy coat layer and to compare this technique to standard i-PRF. Materials and methods Standard high g-force L-PRF and low g-force i-PRF protocols were utilized to separate blood layers. Above the red blood corpuscle layer, sequential 100-μL layers of plasma were harvested (12 layers total; i.e., 1.2 mL, which represents the total i-PRF volume), and 3 layers (3 × 100 μL) were harvested from the red blood cell layer to quantify blood cells. Each layer was then sent for complete blood count (CBC) analysis, and the cell numbers were quantified including red blood cells, leukocytes, neutrophils, lymphocytes, monocytes, and platelets. The liquid PRF that was directly collected from the buffycoat layer following L-PRF protocols was referred to as concentrated PRF (C-PRF).Results The i-PRF protocol typically yielded a 2-to 3-fold increase in platelets and a l.5-fold increase in leukocyte concentration from the 1-to 1.2-mL plasma layer compared to baseline concentrations in whole blood. While almost no cells were found in the first 4-mL layer of L-PRF, a massive accumulation of platelets and leukocytes was found directly within the buffy coat layer demonstrating extremely high concentrations of cells in this 0.3-0.5-mL layer (~20-fold increases). We therefore proposed harvesting this 0.3-to 0.5-mL layer directly above the red blood cell corpuscle layer as liquid C-PRF. In general, i-PRF was able to increase platelet numbers by ~250%, whereas a 1200-1700% increase in platelet numbers could easily be achieved by harvesting this 0.3-0.5 mL of C-PRF (total platelet concentrations of > 2000-3000 × 10 9 cells/L). Conclusion While conventional i-PRF protocols increase platelet yield by 2-3-fold and leukocyte yield by 50%, we convincingly demonstrated the ability to concentrate platelets and leukocytes over 10-fold by harvesting the 0.3-0.5 mL of C-PRF within the buffy coat following L-PRF protocols. Clinical relevance Previous studies have demonstrated only a slight increase in platelet and leukocyte concentrations in i-PRF. The present study described a novel harvesting technique with over a 10-fold increase in platelets and leukocytes that can be further utilized for tissue regeneration.

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Soft tissue regeneration using leukocyte-platelet rich fibrin after exeresis of hyperplastic gingival lesions: two case reports

Cited by 13 publications

References 11 publications

The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes

The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes

Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?

A novel method for harvesting concentrated platelet-rich fibrin (C-PRF) with a 10-fold increase in platelet and leukocyte yields

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