Water stress is the main environmental factor limiting cereal yield in Mediterranean environments. For particular regions, such as the Mediterranean basin, the agroecological conditions are expected to get worse. In response to this challenge attempts are being made to improve crop yield through farmmanagement practices and plant breeding efforts. Here we examine traits that may be used as selection criteria for breeding C 3 cereal crops with improved yield and stability in Mediterranean conditions. Emphasis is made on the potential implications of defining proper selection traits and target environments when adopting biotechnology approaches in breeding programmes. Abbad H, Qariani L, El Jaafari S, Laamarti A, Araus J L. 2003. Comparative relationship of the flag leaf and the ear photosynthesis with the biomass and grain yield of durum wheat under a range of water conditions. Agronomie (In Press). Acevedo E. 1993. Potential of carbon isotope discrimination as a selection criterion in barley breeding. In Stable Isotopes and Plant Carbon/Water Relations, pp. 399-417. Eds J R Ehleringer, A E Hall and G D Farquhar. New York: Academic Press. Amani I, Fischer R A, Reynolds M P. 1996. Canopy temperature depression association with yield of irrigated spring wheat cultivars in hot climate. A, Nachit M M. 1998. Relationships between ash content, carbon isotope discrimination and yield in durum wheat. Australian Journal of Plant Physiology 25:835-842. Araus J L, Brown H R, Febrero A, Bort J, Serret M D. 1993. Ear photosynthesis, carbon isotope discrimination and the contribution of respiratory CO 2 to differences in grain mass in durum wheat. Plant, Cell and Environment 16:383-392. Araus J L, Febrero A, Català M, Molist M, Voltas J, Romagosa I. 1999. Crop water availability in early agriculture: evidence from carbon isotope discrimination of seeds from a tenth millennium BP site on the Euphrates. Global Change Biology 5:233-244. Araus J L, Casadesús J, Bort J, Nachit M M, Villegas D, Aparicio N, Royo C. 2000. Some remarks on ecophysiological traits for breeding. In Durum Wheat Improvement in the Mediterranean Region: New Challenges. Serie A: Séminaires Méditerranéennes, N 40. Options Méditerranéennes, pp. 57-62. Eds C Royo, M M Nachit, N di Fonzo and J L Araus. Zaragoza: IAMZ. Araus J L, Febrero A, Buxó R, Camalich M D, Martín D, Molina F, Rodríguez-Ariza M O, Romagosa I. 1997. Changes in carbon isotope discrimination in grain cereals from different regions of the western Mediterranean Basin during the past seven millennia. Palaeoenvironmental evidence of a differential change in aridity during the late Holocene, Global Change Biology 3:107-118. Araus J L, Villegas D, Aparicio N, García Del Moral L F, El Hani S, Rharrabti Y, Ferrio J P, Royo C. 2003b. Environmental factors determining carbon isotope discrimination and yield in durum wheat under Mediterranean conditions.