Sol-gel glass as a new support matrix in immunoaffinity chromatography

Zühlke, J.; Knopp, Dietmar; Nießner, Reinhard

doi:10.1007/bf00323042

Cited by 44 publications

(20 citation statements)

References 41 publications

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“…From a column technology point of view, this mild thermal condition aspect of solgel chemistry is very important. It significantly simplifies the chemist's job to carry out sol-gel reactions within small-diameter capillaries typically used to prepare capilScheme 2 [158,[173][174][175][176][177], and capillary microextraction [178]. In the following sections we will describe various aspects of sol-gel approach to column technology for CEC.…”

Section: Methodsmentioning

confidence: 99%

Advances in sol‐gel based columns for capillary electrochromatography: Sol‐gel open‐tubular columns

Malik

2002

Electrophoresis

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The development of sol-gel open-tubular column technology in capillary electrochromatography (CEC) is reviewed. Sol-gel column technology offers a versatile means of creating organic-inorganic hybrid stationary phases. Sol-gel column technology provides a general approach to column fabrication for microseparation techniques including CEC, and is amenable to both open-tubular and monolithic columns. Direct chemical bonding of the stationary phase to the capillary inner walls provides enhanced thermal and solvent stability to sol-gel columns. Sol-gel stationary phases inherently possess higher surface area, and thus provide an effective one-step alternative to conventional open-tubular column technology. Sol-gel column technology is applicable to both silica-based and transition metal oxide-based hybrid stationary phases, and thus, provides a great opportunity to utilize advanced material properties of a wide range of nontraditional stationary phases to achieve enhanced selectivity in analytical microseparations. A wide variety of stationary phase ligands can be chemically immobilized on the capillary inner surface using a single-step sol-gel procedure. Sol-gel chemistry can be applied to design stationary phases with desired chromatographic characteristics, including the possibility of creating columns with either a positive or a negative charge on the stationary phase surface. This provides a new tool to control electroosmotic flow (EOF) in the column. Column efficiencies on the order of half a million theoretical plates per meter have been reported for sol-gel open-tubular CEC columns. The selectivity of sol-gel stationary phases can be easily fine-tuned by adjusting the composition of the coating sol solution. Open-tubular columns have significant advantages over their packed counterparts because of the simplicity in column making and hassle-free fritless operation. Open-tubular CEC columns possess low sample capacity and low detection sensitivity. Full utilization of the analytical potential of sol-gel open-tubular columns will require a concomitant development in the area of high-sensitivity detection technology.

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Section: Methodsmentioning

confidence: 99%

Advances in sol‐gel based columns for capillary electrochromatography: Sol‐gel open‐tubular columns

Malik

2002

Electrophoresis

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“…Compared to enzymes, only a few investigations with antibodies have been reported recently. [12][13][14][15][16][17][18] To our knowledge, the usefulness of an immunoadsorbent for the enrichment of analytes (sampling over a distinct period of time) from wet deposition in the field has not been reported thus far. The present article discusses initial results that were gained in the evaluation of a sol-gel -glass based immunoadsorber for trapping PAHs from rainwater.…”

Section: Introductionmentioning

confidence: 93%

“…In preliminary studies in our laboratory, it was found that the silica network can conserve the activity of encapsulated antibodies over several months. 13 But in these experiments the bioceramic matrix was stored in PBS at 4 ЊC. Whether such an adsorber would retain the same level of activity at the elevated temperatures that may occur in a precipitation collector was not known.…”

Section: Influence Of Temperature On the Maximum Capacity Of The Immumentioning

confidence: 99%

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Application of sol-gel glass immunoadsorbers for the enrichment of polycyclic aromatic hydrocarbons (PAHs) from wet precipitation

Scharnweber

Knopp

2000

Field Analyt. Chem. Technol.

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“…53 The same type of column can also be used for the analysis of polycyclic aromatic hydrocarbons. [54][55][56] Alkaline phosphatase and a number of other enzymes can also be effectively entrapped in a sol-gel glass, and they retain approximately 30% of their enzymatic activities. 57 Zusman et al immobilized protein A in a sol-gel matrix prepared from a mixture of TMOS, to purify immunoglobulin G from sheep sera by affinity chromatography.…”

Section: ·1·1 Packed Columnsmentioning

confidence: 99%

Integration of Biomolecules into analytical Systems by Means of Silica Sol-Gel Technology

Sakai‐Kato

Ishikura

2009

ANAL. SCI.

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Biomolecules, especially large polymeric molecules such as enzymes and antibodies, mediate various biological functions, including biochemical reactions and molecular recognition, with high reactivity, efficiency, selectivity and accuracy. Many researchers have investigated methods to take advantage of these characteristics in analytical devices. One way to accomplish this is to immobilize biomolecules in the devices. For example, biomolecules have been immobilized by means of silica sol-gel technology and used for basic research in the food and pharmaceutical industries. Proteins encapsulated by this method retain their structure and biological activity for a prolonged period. This review describes methodologies for immobilization of biomolecules and the applications of sol-gel technology to analytical devices, especially flow-through systems.

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Sol-gel glass as a new support matrix in immunoaffinity chromatography

Cited by 44 publications

References 41 publications

Advances in sol‐gel based columns for capillary electrochromatography: Sol‐gel open‐tubular columns

Advances in sol‐gel based columns for capillary electrochromatography: Sol‐gel open‐tubular columns

Application of sol-gel glass immunoadsorbers for the enrichment of polycyclic aromatic hydrocarbons (PAHs) from wet precipitation

Integration of Biomolecules into analytical Systems by Means of Silica Sol-Gel Technology

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