Solid Ehrlich carcinoma reproduces functional and biological characteristics of cancer cachexia

Frajacomo, Fernando Tadeu Trevisan; Padilha, Camila S.; Marinello, Poliana Camila; Guarnier, Flávia Alessandra; Cecchini, Rúbens; Duarte, José Alberto; Deminice, Rafael

doi:10.1016/j.lfs.2016.08.009

Cited by 36 publications

(29 citation statements)

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“…Ehrlich's tumor is a spontaneous murine mammary adenocarcinoma adapted to ascitic form and carried in mice by serial intraperitoneal passages (Cassali et al 2006;Calixto-Campos et al 2013). This tumor was described for the first time by Ehrlich and Apolant (1905) and is used for testing antineoplastic drugs, cancer pain and cachexia (Calixto-Campos et al 2013;Frajacomo et al 2016;Albiero et al 2016). The Ehrlich's tumor model is widely used in experimental cancer studies due to its versatility.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

et al. 2019

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Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.

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Section: Introductionmentioning

confidence: 99%

“…The Ehrlich's tumor model is widely used in experimental cancer studies due to its versatility. It evolves into an ascitic form when inoculated by intraperitoneal route, and into a solid form when inoculated subcutaneously, and is able to grow in any type of mice (Calixto-Campos et al 2013;Albiero et al 2016;Frajacomo et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

et al. 2019

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“…For this reason, EST was successfully used as a model to study tumor development and progression and to evaluate the therapeutic potential of new anti-cancer agents [20]. Moreover, this model was successfully used by several researchers to study the anticancer effects of natural products, synthetic chemicals, and nanoparticles [19,[21][22][23][24]. Recently, EST was used as a tumor model to investigate the anti-cancer effects of sulforaphane (an active ingredient of cruciferous vegetables such as broccoli and cabbage) [25] and Avenanthramides (a group of phenolic alkaloids found mainly in oats and white cabbage) [26].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Sharawi

2020

BMC Complement Med Ther

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Background: The anti-cancer effect of the halophyte Arthrocnemum indicum, a member of Arthrocnemum family of salt-tolerant plants, was evaluated against colorectal cancer cell, CaCo2. However, the anti-cancer effect of another halophyte Arthrocnemum machrostachyum was not investigated yet. Herein, the anticancer effect of A. machrostachyum methanolic extract (AME) was evaluated against Ehrlich solid tumor (EST) in mice and the potential mechanism of action was also studied. Methods: Male Swiss albino mice (n = 28) were randomly divided into 4 groups (n = 7/group). Group 1 (negative control group); group 2 (EST) injected intramuscularly by 0.2 mL Ehrlich ascitic carcinoma (2 × 10 6 cells); and groups 3 and 4 injected intratumorally with AME (180 and 360 mg/kg body weight, respectively) at D12 trice weekly for 2 weeks. Gene expression, protein expression, DNA damage, and TNFa level in tumors were determined by real-time PCR, western blot, comet assay, and Elisa, respectively. Results: Treatment with AME induced anti-tumor effects against EST as indicated by 1) notable reduction in tumor size; 2) elevation in tissue necrosis and apoptosis, as confirmed histologically; 3) increased DNA fragmentation; 4) decreased expression of the apoptotic genes (p53, Bax and caspase 3), and increased expression of the antiapoptotic marker Bcl2; 5) significantly upregulated cell cycle regulatory genes Cdc2 and connexin26, and; 6) decreased TNFa levels in tumor tissues. Interestingly, a high dose of AME exhibited a more potent anti-tumor effect against EST. Conclusion: These findings indicate that AME has a potent antitumor effect against EST and could be used as an adjuvant to anticancer drugs to combat tumor, but after application of further confirmatory clinical trials.

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“…Ehrlich tumor is a spontaneous murine mammary adenocarcinoma adapted to the ascitic form and inoculated in mice by serial intraperitoneal passages [9,10]. This tumor model is widely used in experimental cancer studies due to its versatility; it is able to adapt to an ascitic form when inoculated by the intraperitoneal route or a solid form when inoculated by the subcutaneous route [9,11,12].…”

Section: Introductionmentioning

confidence: 99%

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

Rodrigues

Albiero

Nery

et al. 2019

Sci Med

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AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.

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Solid Ehrlich carcinoma reproduces functional and biological characteristics of cancer cachexia

Cited by 36 publications

References 38 publications

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

Therapeutic effect of Arthrocnemum machrostachyum methanolic extract on Ehrlich solid tumor in mice

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

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