Somatic embryogenesis of Turkish Cyclamen persicum Mill

Koçak, Mustafa; İzgü, Tolga; Sevindik, Başar; Tütüncü, Mehmet; Çürük, Pembe; Şimşek, Özhan; Kaçar, Yıldız Aka; Silva, Jaime A. Teixeira da; Mendi, Yeşim Yalçın

doi:10.1016/j.scienta.2014.03.044

Cited by 22 publications

(18 citation statements)

References 22 publications

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“…Ovaries were divided into four equally sized pieces and ovules were removed with a surgical blade from ovaries and placed onto callus induction medium (CIM) [25 ovules/Petri dish (90 9 15 mm), 15 leaf segments and petiole segments/Petri dish, 8 pieces of ovary parts/Petri dishes and five replicates of each explant type], as described by Schwenkel and Winkelmann (1998) and Kocak et al (2014). Leaf (approx.…”

Section: Explant Preparation and Callus Inductionmentioning

confidence: 99%

“…Callus was incubated at 25 ± 2°C in constant darkness and subcultured every 4 weeks, as described by Kocak et al (2014). To convert ELSs into plantlets, mature ELSs from different explant types were transferred to magenta boxes (76 9 76 9 100 mm) containing PGR-free CIM, 30 % sucrose, 3.5 g l -1 gelrite, at pH 5.7, and maintained for 2 months under a 16-h photoperiod and 75 lmol m -2 s -1 provided by cool white fluorescent lamps at 24 ± 1°C.…”

Section: Differentiation and Germination Of Elssmentioning

confidence: 99%

“…Regeneration from callus derived from different explants, such as tubers (Loewenberg 1969;Geier 1977;Bian et al 2008Bian et al , 2010, leaves (Wicart et al 1984;Otani and Shimada 1991;Dillen et al 1996;Terakawa et al 2008;Gou et al 2008;You et al 2008;Prange et al 2010b;Naderi et al 2012;Kocak et al 2014), petioles (Wicart et al 1984;Kiviharju et al 1992;You et al 2008;Naderi et al 2012; Kocak et al ) and 4 mg l -1 2,4-D ? 0.1 mg l -1 Kin (Jalali et al 2010b).…”

Section: Callus Induction From Different Explant Types and Pgrs Of Enmentioning

confidence: 99%

“…In this study we obtained different ratio of callus formation from leaf explant in all endemic species (92 % for C. pseudibericum, 56 % for C. cilicium, 4 % for C. mirabile, 59 % for C. parviflorum; Tables 1, 2, 3 and 4, respectively). Kocak et al (2014) investigated the potential of somatic embryogenesis from different explants (ovules, divided ovary parts, leaves and petiole segments) of 15 distinct wild C. persicum genotypes. Explants were cultured on CIM containing 2.0 mg l -1 2,4-D and 0.8 mg l -1 2iP to induce callus: all explants formed regenerative and nonregenerative callus, and the former was generally light brown, soft, friable and separable.…”

Section: Differentiation Of Elss (Cim With Pgrs)mentioning

confidence: 99%

“…(Schwenkel and Grunewaldt 1988;Al-Majathoub 1999;Al-Majathoub and Karam 2000;Karam and Al-Majathoub 2000a, b;Abu-Qaoud 2004;Jalali et al 2012;Nhut et al 2012), but also for C. mirabile (Yamaner and Erdag 2008). Somatic embryogenesis has been found to be an efficient system for the in vitro propagation of C. persicum ; Motoyasu and Takiko 1991;Kreuger et al 1995;Schwenkel and Winkelmann 1998;Ruffoni et al 1998;Hohe et al 1999a, b;Winkelmann et al 2003;Winkelmann and Serek 2005;Winkelmann et al 2006;Naderi et al 2012;Kocak et al 2014). Only few reports have dealt with somatic embryogenesis or the formation of embryo-like structures (ELSs) in wild Cyclamen species, namely C. cilicium and C. mirabile (Furukawa et al 2001(Furukawa et al , 2002Seyring et al 2009;Prange et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

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Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

İzgü

Sevindik

Çürük

et al. 2016

Plant Cell Tiss Organ Cult

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In this study, embryo-like structures (ELSs) were induced in four endemic Turkish Cyclamen species (C. cilicium Boiss. et Heldr., C. parviflorum Pobed., C. mirabile Hildebr. and C. pseudibericum Hildebr.) in the presence of 13 combinations of two plant growth regulators (PGRs) (2,4-dichlorophenoxyacetic acid and 6-(c,cdimethylallylamino)purine) and four explant types (ovules, ovaries, leaves and petioles). The ratio of callus induction, different stages of ELS formation and the conversion of ELSs to plantlets were quantified. The most effective explant types for callus induction were leaves (56 % for C. cilicium and 59 % for C. parviflorum) and petioles (80 % for C. mirabile and 100 % for C. pseudibericum). Callus growth from the leaves and petioles of C. cilicium was 30 days earlier than that of C. mirabile and C. pseudibericum. In contrast, most callus formed from the petioles of C. pseudibericum (100 %) in medium with 2.5 mg l -1 2,4-D and 1 mg l -1 2iP. The highest number of ELSs was obtained succesfully from petioles (2.5 mg l -1 2,4-D and 1 mg l -1 2iP) and ovaries (2.5 mg l -1 2,4-D and 0.5 mg l -1 2iP) of C. pseudibericum, in 39 % and as 32 % of explants, respectively. The percentage conversion of ELSs to plantlets was 38, 31, 16 and 15 % for C. mirabile, C. cilicium, C. pseudibericum and C. parviflorum, respectively. The plantlets were successfully acclimatized in the greenhouse with 54, 70, 63 and 25 % of C. cilicium, C. mirabile, C. pseudibericum and C. parviflorum plantlets, respectively surviving after transfer to ex vitro conditions. This paper describes a unique, reliable and consistent protocol for the induction of ELSs from four endangered endemic Turkish Cyclamen species, opening up the possibility of preserving these valuable genetic resources in vitro and also other applied biotechnologies that rely on a stable embryogenic or callus-based protocol.

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Section: Explant Preparation and Callus Inductionmentioning

confidence: 99%

Section: Differentiation and Germination Of Elssmentioning

confidence: 99%

Section: Callus Induction From Different Explant Types and Pgrs Of Enmentioning

confidence: 99%

Section: Differentiation Of Elss (Cim With Pgrs)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

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Sevindik

Çürük

et al. 2016

Plant Cell Tiss Organ Cult

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Comparative anatomical survey of Cyclamen L. (Primulaceae) taxa and their traditional medicinal uses in Turkey

Zare

Çilden

Çelemli

2022

Microscopy Res & Technique

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The genus Cyclamen is native to Turkey, where it is represented by 12 perennial tuberous geophytic taxa. In this study, a detailed anatomical description of all Cyclamen taxa found in Turkey is provided for the first time. Tuber, root, petiole, and leaf cross-sections were stained with Astra blue and safranin, and tuber slides were also stained with sartur. Tubers of these taxa used by local people as a treatment for infertility were separately investigated. Stomatal index values and epidermis cell features of the studied taxa were measured. Indumentum features of the leaves were described for the first time. Petiole and leaf anatomy and particularly stomatal density, size, and index, provided valuable evidence for species identification. Although the anatomical features of these taxa are not sufficient as diagnostic characters, they can be used together with morphological features.

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Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant

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et al. 2022

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Somatic embryogenesis of Turkish Cyclamen persicum Mill

Cited by 22 publications

References 22 publications

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

Comparative anatomical survey of Cyclamen L. (Primulaceae) taxa and their traditional medicinal uses in Turkey

Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant

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