Some Aspects of PCB Metabolism by Horseradish Cells

Macková, Martina; Chroma, Ludmila; Kučerová, Petra; Burkhard, J.; Demnerova, Kateřina; Macek, Tomáš

doi:10.1080/15226510108500067

Cited by 17 publications

(7 citation statements)

References 18 publications

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“…It is important to note that the manifestation of this toxic effect was dependent on the dose of phenol to which the plants were exposed. Plant peroxidases are oxidoreductive enzymes related to the metabolism of several organic contaminants, including phenols [2,9,[27][28][29]. The level and isoenzyme pattern of peroxidases can be altered by environmental stress and these enzymes are frequently used as non-specific biomarkers of environmental pollution.…”

Section: Resultsmentioning

confidence: 99%

Removal of Phenol by Alfalfa Plants (Medicago sativa L.) Grown in Hydroponics and its Effect on Some Physiological Parameters

et al. 2002

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SummaryThe plant-assisted removal of phenol, with special emphasis on the effects of this compound on some plant's physiological parameters, was investigated. Hydroponic cultures of alfalfa (Medicago sativa L., var. Romagnola) were employed as a model system. These cultures were exposed to two phenol concentrations: 100 and 500 mg/l. A first order kinetic approach was used to describe the removal of phenol from the solution. After 30 days of cultivation, the initial amount of phenol (100 mg/l) was reduced to non-detectable levels in the presence of plants. In the absence of plants, 20% of phenol remained in the solution. The half-life of phenol was reduced from 7.2 to 4.5 days in the presence of plants. After 25 days, the initial amount of 500 mg/l of phenol was reduced to non-detectable levels in the presence of plants not previously exposed to phenol and to approximately 20% with plants previously exposed to the contaminant. In the absence of plants, almost 40% remained in the solution. The presence of plants reduced the half-life of phenol from 18.3 days to 10.4 in the case of plants previously exposed and to 7.8 days in the case of plants without previous contact. Chlorophyll contents in alfalfa leaves of plants exposed to 100 mg/l of phenol were similar to those of control plants and a decrease in total chlorophyll content was observed when plants were exposed to 500 mg/l of phenol. The activity of soluble peroxidases of the roots increased in the presence of 100 mg/l of phenol but the amount of 500 mg/l had a negative effect on the peroxidase fraction. No changes were observed in the case of the ionically-bound cell wall fraction. The growth index of the plants exposed to 100 mg/l of phenol was comparable to that of non-exposed plants, while this parameter was negatively affected in the case of plants exposed to 500 mg/l of phenol. Although alfalfa plants were able to survive an exposure to 500 mg/l of phenol, their physiological parameters and their removal capacity were negatively affected.

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Section: Resultsmentioning

confidence: 99%

Removal of Phenol by Alfalfa Plants (Medicago sativa L.) Grown in Hydroponics and its Effect on Some Physiological Parameters

et al. 2002

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“…A positive correlation between the peroxidase content of plant tissues and the disappearance of polychlorinated biphenyls and PAHs has been reported (Kucerová et al 1998(Kucerová et al , 1999. A recently published work 240092.pgs 9/17/02, 10:10 AM 182 (Macková et al 2001) presents more data supporting the theory that peroxidases may play a role in the metabolism of aromatic molecules. Taking into account a possible role of peroxidases in the detoxification of PAHs or in stress responses to the presence of organic contaminants, we evaluated the peroxidase activity of roots of alfalfa plants during the removal assay.…”

Section: Resultsmentioning

confidence: 76%

Some Physiological, Microbial, and Toxicological Aspects of the Removal of Phenanthrene by Hydroponic Cultures of Alfalfa (Medicago sativaL.)

Flocco¹,

Lobalbo

Carranza³

et al. 2002

International Journal of Phytoremediation

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Alfalfa (Medicago sativa L.) and other plants bearing an important root system have been shown to be effective in the removal of organic compounds, including polycyclic aromatic hydrocarbons (PAHs). Phenanthrene is one of the main contaminants arising from the petrochemical industry and is included in the USEPA's list of priority toxic pollutants. Hydroponic cultures of alfalfa were employed as a model system to evaluate their capability of removing phenanthrene and to study the plantpollutant interaction without the interference of a soil matrix. The removal of phenanthrene was followed over a period of 30 days. The half-life of phenanthrene in hydroponics (initial concentration 50 mg L -1 ) was reduced 2.7 times when plants were present. Growth index, chlorophyll content of leaves, and peroxidase activity of the roots of plants exposed to phenanthrene were lower than the corresponding values of nonexposed plants. Phenanthrene produced an acute negative effect on the total bacterial counts but also caused an increase in degraders/total bacteria ratio. The Ames Salmonella plate incorporation assay was employed to screen for potential genotoxic metabolites, which could be generated by metabolic activation of the parent compound. None of the samples exhibited a positive response. While lack of a positive response to this test is not a definitive evidence of the absence of genotoxic substances, these results suggest that the plant-assisted removal of phenanthrene merits further investigation.

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“…Earlier phytoremediation studies have been carried out using in vitro cell and tissue culture techniques and genetic engineering (Mackova et al 2001;Eapen and D'Souza 2005;Padmavathiamma and Loretta 2007;Guillon et al 2008) which offer unique opportunities that complement and extend the existing options. Against this background, attempts have been made in the present work on the potential of in vitro grown Sesuvium plantlets to degrade the sulfonated dye, Green HE4B (GHE4B), with an insight into the involvement of different enzymes and prediction of metabolic pathway responsible for the biotransformation of these recalcitrant compounds.…”

Section: Introductionmentioning

confidence: 99%

Sesuvium portulacastrum (L.) L.: a potential halophyte for the degradation of toxic textile dye, Green HE4B

Patil

Lokhande

Suprasanna

et al. 2011

Planta

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Sesuvium portulacastrum is a common halophyte growing well in adverse surroundings and is exploited mainly for the environmental protection including phytoremediation, desalination and stabilization of contaminated soil. In the present investigation, attempts have been made on the decolorization of a toxic textile dye Green HE4B (GHE4B) using in vitro grown Sesuvium plantlets. The plantlets exhibited significant (70%) decolorization of GHE4B (50 mg l(-1)) that sustain 200 mM sodium chloride (NaCl) within 5 days of incubation. The enzymatic analysis performed on the root and shoot tissues of the in vitro plantlets subjected to GHE4B decolorization in the presence of 200 mM NaCl showed a noteworthy induction of tyrosinase, lignin peroxidase and NADH-DCIP reductase activities, indicating the involvement of these enzymes in the metabolism of the dye GHE4B. The UV-visible spectrophotometer, HPLC and Fourier Transform Infrared Spectroscopy (FTIR) analyses of the samples before and after decolorization of the dye confirmed the efficient phytotransformation of GHE4B in the presence of 200 mM NaCl. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis of the products revealed the formation of three metabolites such as p -amino benzene, p -amino toluene and 1, 2, 7-amino naphthalene after phytotransformation of GHE4B. Based on the FTIR and GC-MS results, the possible pathway for the biodegradation of GHE4B in the presence of 200 mM NaCl has been proposed. The phytotoxicity experiments confirmed the non-toxicity of the degraded products. The present study demonstrates for the first time the potential of Sesuvium for the efficient degradation of textile dyes and its efficacy on saline soils contaminated with toxic compounds.

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Some Aspects of PCB Metabolism by Horseradish Cells

Cited by 17 publications

References 18 publications

Removal of Phenol by Alfalfa Plants (Medicago sativa L.) Grown in Hydroponics and its Effect on Some Physiological Parameters

Removal of Phenol by Alfalfa Plants (Medicago sativa L.) Grown in Hydroponics and its Effect on Some Physiological Parameters

Some Physiological, Microbial, and Toxicological Aspects of the Removal of Phenanthrene by Hydroponic Cultures of Alfalfa (Medicago sativaL.)

Sesuvium portulacastrum (L.) L.: a potential halophyte for the degradation of toxic textile dye, Green HE4B

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