Some Clinical and Epizootological Observations of Infectious Sialoadenitis in Rats

Utsumi, Kenta; Maeda, Toshiro; Tazaki, Hiroshi; Fujiwara, Kôsaku

doi:10.1538/expanim1978.27.3_283

Cited by 19 publications

(17 citation statements)

References 3 publications

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“…However, there seemed to be certain problems in antigenicity and reactivity [1]. In Japan, MHV antigen prepared from infected mouse liver has been widly used [4,15,17] and anti-MHV-2 antibody detected in conventionl rat breeder or user colonies was indicative of SDAV infection [4,14]. Using enzymeliked immunosorbent assay with SDAV-TG, Parker's coronavirus (PCV) 8190, MHV-S and MHV-NuU by Machii et al [13] revealed lower reactivities of MHV-S and MHV-NuU antigens for detection of anti-SDAV antibody in rats.…”

Section: Resultsmentioning

confidence: 99%

“…is widely conducted for detection of infectious diseases in breeding and test colonies of laboratory rats and mice [1,3,4,9,10,13,[15][16][17] . For sialodacryoadenitis virus (SDAV), complement fixation (CF) reaction with mouse hepatitis virus (MHV), which shares common antigens with SDAV, has been routinely used in Japan [4,15,17], while antigenic relatedness between SDAV and MHV has not been thoroughly investigated.…”

Section: Seromonitoringmentioning

confidence: 99%

“…For sialodacryoadenitis virus (SDAV), complement fixation (CF) reaction with mouse hepatitis virus (MHV), which shares common antigens with SDAV, has been routinely used in Japan [4,15,17], while antigenic relatedness between SDAV and MHV has not been thoroughly investigated. Of SDAV antigen used in the U. S. A. certain problems in its preparation procedures and specificity have been described [1,2,161.…”

Section: Seromonitoringmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Serum Antibodies Using LBC Cell-grown Sialodacryoadenitis (SDA) Virus

Utsumi

Ishikawa

Yokota

et al. 1991

Experimental Animals

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Section: Resultsmentioning

confidence: 99%

Section: Seromonitoringmentioning

confidence: 99%

Section: Seromonitoringmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Serum Antibodies Using LBC Cell-grown Sialodacryoadenitis (SDA) Virus

Utsumi

Ishikawa

Yokota

et al. 1991

Experimental Animals

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“…SDAV can also cause reproductive disorders and behavioral changes in the infected animals. Serologic surveys indicate that coronavirus infections are common in laboratory rats housed in research facilities (11,16), and several outbreaks of SDAV in rat colonies have been reported (2,6,12,22,32; J. Storz, personal communication). Therefore, SDAV is an important viral pathogen in comparative laboratory medicine.…”

mentioning

confidence: 99%

Primary Structure of the Sialodacryoadenitis Virus Genome: Sequence of the Structural-Protein Region and Its Application for Differential Diagnosis

Yoo

Pei

Christie

et al. 2000

Clin Diagn Lab Immunol

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Sialodacryoadenitis virus (SDAV) is a coronavirus that is commonly found in laboratory rats and that causes sialodacryoadenitis and respiratory illness. We cloned and sequenced the 3 terminal 9.8 kb of the genomic RNA and analyzed the structure of the viral genome. As with mouse hepatitis coronaviruses (MHVs), the SDAV genome was able to code for a spike protein, a small membrane protein, a membrane-associated protein, and a nucleocapsid protein. In addition, the hemagglutinin-esterase gene capable of encoding a protein of 439 amino acids (aa) was identified. The putative functional site for acetylesterase activity was present in the HE protein as Phe-Gly-Asp-Ser (FGDS), suggesting that the SDAV HE protein might have retained the esterase activity. Immediately upstream of the HE gene and downstream of the polymerase 1b gene, the NS2 nonstructural-protein gene was identified with a coding capacity of 274 aa. A motif of UCUAAAC was identified as a potential transcription signal for subgenomic mRNA synthesis. Large insertions of 172, 127, and 44 aa were detected in the N-terminal half of the predicted S protein of SDAV when its sequence was compared to the sequences of MHV 2, MHV JHM, and MHV A59, respectively. The sequence information on the SDAV S-protein gene was applied to a differential diagnostic PCR to detect and distinguish the rat coronavirus from mouse coronaviruses. This is the first report on the comprehensive genetic information of any rat coronavirus.Sialodacryoadenitis virus (SDAV) is distributed worldwide in laboratory rats. SDAV infects the lacrimal and salivary glands and the upper and lower respiratory tracts of rats, causing the clinical manifestations of enlarged salivary glands, sialoadenitis, dacryoadenitis, rhinitis, tracheitis, and bronchoalveolitis (3, 9, 10). SDAV can also cause reproductive disorders and behavioral changes in the infected animals. Serologic surveys indicate that coronavirus infections are common in laboratory rats housed in research facilities (11,16), and several outbreaks of SDAV in rat colonies have been reported (2,6,12,22,32; J. Storz, personal communication). Therefore, SDAV is an important viral pathogen in comparative laboratory medicine.SDAV is antigenically related to the mouse hepatitis virus (MHV) serogroup of the family Coronaviridae in the order of Nidovirales (20). The MHV serogroup includes Parker's rat coronavirus (PRCV), bovine coronavirus (BCV), and human coronavirus (HCV) strain OC43. Coronavirus is an enveloped virus with a single-stranded positive-sense RNA genome of approximately 31 kb. The 5Ј-most 22 kb of the coronavirus genome encodes the nonstructural RNA-dependent RNA polymerase, while all the structural proteins are encoded in the 3Ј terminal 9 kb of the genome (15). Although a large amount of genetic information has been accumulated for MHV and other coronaviruses, such information is not available for any rat coronavirus, mainly due to the difficulty with propagation of the virus in cell cultures. Percy and coworkers (25) reported that a su...

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“…In breeding colonies infected with sialodacryoadenitis virus (SDAV) considerable disorders in estrous cycle [8] and decreased reproductive performance [9] of female rats were reported.…”

mentioning

confidence: 99%

Reproductive Disorders in Female Rats Infected with Sialodacryoadenitis Virus

Utsumi

Maeda

Yokota

et al. 1991

Experimental Animals

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Some Clinical and Epizootological Observations of Infectious Sialoadenitis in Rats

Cited by 19 publications

References 3 publications

Detection of Serum Antibodies Using LBC Cell-grown Sialodacryoadenitis (SDA) Virus

Detection of Serum Antibodies Using LBC Cell-grown Sialodacryoadenitis (SDA) Virus

Primary Structure of the Sialodacryoadenitis Virus Genome: Sequence of the Structural-Protein Region and Its Application for Differential Diagnosis

Reproductive Disorders in Female Rats Infected with Sialodacryoadenitis Virus

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