Some properties and applications of monoclonal antibodies

Edwards, Paul A.W.

doi:10.1042/bj2000001

Cited by 70 publications

(50 citation statements)

References 63 publications

(65 reference statements)

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“…Many laboratories have been raising monoclonal antibodies to cell-surface antigens of human epithelia and epithelial tumours in the hope of applying them to the diagnosis, detection and therapy of malignancy (reviews: monoclonal antibodies in general, Edwards, 1981;used in pathology, Neville et al, 1982;Damjanov & Knowles, 1983; in therapy, Levy & Miller, 1983; antigens on tumour cells, Lloyd, 1983). Perhaps the most interesting observation that has come out of this work is that when a tumour or normal epithelium is stained with a given antibody only some of the cells in the tumour or epithelium bind the antibody.…”

mentioning

confidence: 99%

Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Edwards¹

1985

Br J Cancer

110

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confidence: 99%

Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Edwards¹

1985

Br J Cancer

110

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“…Si milar methods to those used for studying cell surface antigen systems over many years [3], and more recent ly, have been applied to a number of systems contain ing inaccessible complex antigen mixtures [1], Despite the manifest advantages of this system, however, it must be acknowledged that the technique as employ ed in the current studies is subject to several limita tions. Firstly, only those antigenic components which radiolabel readily, and retain their immunoreactivity, can be detected.…”

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Antibody Response Profiles to Components of Rye Grass Pollen Extract in Various Strains of Mice

Moran

Standring

Henderson

1982

Int Arch Allergy Immunol

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Using a combination of immunoprecipitation and polyacrylamide gel electrophoresis techniques, patterns of antibody responses to a number of components in rye pollen extract have been studied in hyperimmune mouse sera. Common laboratory strains of mice were investigated, including AKR, C₃H/H_e, SwR, Ist, Asn, Balb/c, C₅₇BL₁₀, DBA₂ and BD1: F1 hybrids. All strains responded vigorously to the immunisation protocol used (rye pollen extract/alum followed by a booster dose in aqueous media) when tested with non-discriminating assay procedures such as haemagglutination or passive cutaneous anaphylaxis directed against the whole rye pollen extract. However, markedly variable antibody responses were observed between strains and between murine and human sera when considered in relation to individual rye extract components. Particularly noteworthy was the relatively poor response of all mice to the low molecular weight rye antigen component (11,000 daltons). This material was readily detected by human sera derived from pollenosis patients. Notable also was the fact that no single rye component was detected by all mice sera. These results illustrate the complex serological response induced by heterogeneous antigen mixtures, such as grass pollen extracts.

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“…Edwards (1981) has emphasized that monoclonal antibodies are often able to bind other antigenic determinants having enough structural similarity to allow recognition. The question of multiple specificity of monoclonal anti¬ bodies has been reviewed by Lane & Koprowski (1982), who cited several examples of monoclonal antibodies binding to apparently unrelated antigens.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies recognize a cell surface marker of epithelial differentiation in the rabbit reproductive tract

Lampelo¹,

Anderson²,

Bullock³

1986

Reproduction

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Summary. Monoclonal antibodies against the cell surface were produced by immunizing mice with endometrial scrapings prepared from 6-day pregnant rabbits. Spleen cells from an immune mouse were fused with myeloma cells and cultured by standard hybridoma technology methods. Hybridoma supernatants were screened for reaction with the apical epithelial surface by immunohistochemistry on frozen sections of uterus from 6-day pregnant rabbits, and positive colonies were cloned by limiting dilution. Ascites fluid was produced in mice from hybridoma clones that gave a consistent pattern of apical epithelial surface staining through 6 sub-clonings. Antibodies in the ascites fluid were tested by immunohistochemistry on frozen sections of uterus, oviduct, lung, liver and kidney from nonpregnant or 6-day pregnant rabbits. At a dilution of 1:5000, the antibodies recognized an antigen that was specific to the apical surface of luminal but not glandular epithelium of the 6-day pregnant uterus and could not be detected in the nonpregnant uterine epithelium. At higher concentrations of antibody (1:100 to 1:1000), crossreaction was seen with antigens in stromal and myometrial cells of pregnant and nonpregnant uterus. At a dilution of 1:5000, the antibody also crossreacted with some components of lung, liver and kidney but without discriminating between the two reproductive states. In the oviduct, staining of the surface epithelium was specific to the pregnant state. We conclude that this monoclonal antibody has a high affinity for a luminal epithelial cell surface antigen in the reproductive tract of the pregnant rabbit and shows multiple organ reactivity with other tissues that is not affected by pregnancy. This antigen will provide a useful cell surface marker of epithelial differentiation in the progestational reproductive tract.

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Some properties and applications of monoclonal antibodies

Cited by 70 publications

References 63 publications

Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Antibody Response Profiles to Components of Rye Grass Pollen Extract in Various Strains of Mice

Monoclonal antibodies recognize a cell surface marker of epithelial differentiation in the rabbit reproductive tract

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