Some Properties of Cucumber Fruit Streak Virus

Abstract

An isometric virus c. 30 nm in diameter with a single RNA species (mol.wt 1.45 X isolated from cucumber plants from the island of Crete (Greece) is described under the name of cucumber fruit streak virus (CFSV). The most evident symptom on naturally infected plants consisted of longitudinal chlorotic streak of the fruits. In glasshouse, the virus was soil-transmi'tted to C. sativuSy and, mechanically, to a wide range of herbaceous hosts, most of which were infected only locally.Purified virus preparations sedimented as a single component with sedimentation coefficient of 132S. At equilibrium these preparations were homogeneous in CsCl gradient.^ but formed two bands in Cs^SO^ gradients. Virus particles were stabilized by forces involving divalent cations, pH-dependent bonds and salt links between protein and RNA. Although some of the properties of CFSV are similar to those of other small spherical viruses with single RNA species there are differences which do not allow for the assignment of 'the virus to any of established taxonomie group of plant viruses. Zusammenfassung Einige Eigenschaften des Cucumber fruit streak virusDas aus Gurkenpflanzen auf der Insel Kreta (Griechenland) isolierte isometrische Virus (ungefahr 39 nm Durchmesser, mit einer einzigen RNA-Art [Molekulargewicht 1,45 X lQe]) wird unter der Bezeichnung ,,Cucumber fruit streak virus" (CFSV) besdirieben. Das offcnsichtlichste Symptom bei natiirlich infizierren Pflanzen bestand aus langslaufenden chlorotischen Streifen an den Friichten. Im Gewachshaus wurde das Virus auf C. sativus durch die Erde und auf ein weites Spektrum anderer krautartiger Wirtspflanzen mechanisch ubertragen. Die letzjtgenannten Pflanzen waren meist nur ortlidi angegriffen. U.S. Copyright Clearance Center Code Statement: 0031-948l/83/0602-0149$02.50/0 GM.I.ITKLLI, VOVLAS and AVGELIS Virusprapiiratc scdimonticrtcn als einzigc Komponenite mit einem Sedimentations-kocfU/iontcn von 132S. Ausgcwogcn waren diese Praparate in CsCl-Gradientcn homogen, jcdoch biidcten sie in Cs.,SO^-Gradienten zwei Banden. Die Korngrofie der Viren wurde durdi Kriiftc, an denen divalen^te Kationen, pH-bedingtc Bindungen und Salzverbindungen zwischen dciii Protein und RNA beteiligt waren, stabilisiert. Obwohl einige Eigenschaften JL'S Cl'SV L\cn anJcri'ii kleinsphiirischen Viren mit einer einzigen RNA-Art ahneln, sind die Untcrsdiicdc doch zu grof^, um dieses Virus einer taxonomlsch bestehcnden Gruppe der Pflanzenviren zuzuordnen.

Characterisation of cucumber leaf spot virus isolated from recycled irrigation water of soil‐less cucumber cultures

“…2C). In contrast to the CLSV strains described by Gallitelli et al (1983), the Israeli isolate differed in host range, failing to infect Citrullus vulgaris , Cucumis melo , Cucurbita pepo and N. rustica but did infect N. tabacum cv. Samsun and White Burley.…”

“…The virus infects cucumber crops in addition to various test plants, e.g. Gomphrena globosa , Chenopodium amaranticolor and Petunia hybrida (Weber et al , 1982; Gallitelli et al , 1983). Infected cucumber leaves show necrotic spots and the plants are stunted (Weber, 1986).…”

Molecular and Serological Identification of Cucumber leaf spot virus in Iran

“…The experimental host range is moderately wide (Gallitelli et al. ). Aureusviruses have isometric particles c .…”

“…Aureusviruses are readily transmitted by sap inoculation, and some species are reported to be soil-borne. The experimental host range is moderately wide (Gallitelli et al 1983). Aureusviruses have isometric particles c. 30 nm in diameter and a structure similar to other members of the family Tombusviridae, to which this genus belongs.…”

Aureusvirus, a novel genus in the family Tombusviridae

“…In the meantime, molecular investigations of cucumber leaf spot virus (CLSV), a definitive species in the genus Carmovirus [5,9], also known under the name of cucumber fruit streak virus [3], revealed that this virus had the same genome size, structure, and organization as PoLV [4], thus representing a possible additional member of the genus.…”