Some properties of spinach chloroplast fractions obtained by digitonin solubilization

Cederstrand, Carl

doi:10.1016/0926-6585(66)90294-9

Cited by 40 publications

(9 citation statements)

References 6 publications

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“…Further measurements confirmed the above results (39,116,197), whereas other detergents like Triton X-lOa (112) or sodium desoxycholate (DOC) (23) could be used also. With the latter a good separation of partial fractions-as judged on the basis of -196°C fluorescence spectra-was ob tained.…”

Section: Fluorescence Emission Spectra Of Chlorophyllssupporting

confidence: 68%

Fluorescence in Relation to Photosynthesis

Goedheer

1972

Annu. Rev. Plant. Physiol.

159

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Section: Fluorescence Emission Spectra Of Chlorophyllssupporting

confidence: 68%

Fluorescence in Relation to Photosynthesis

Goedheer

1972

Annu. Rev. Plant. Physiol.

159

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“…The membranes obtained were frozen in liquid nitrogen after addition of glycerol to 10%, and stored at -80°C. The purity of the preparations of thylakoid membrane enriched with one or the other photosystem was tested using the low temperature chlorophyll fluorescence spectra measured at 77°K with a spectrofluorimeter (Hitachi, Japan) applying a monochromatic exciting light at 435 nm (Cederstrand and Govindjee 1966).…”

Section: Methodsmentioning

confidence: 99%

Carbonic anhydrase activity in Arabidopsis thaliana thylakoid membrane and fragments enriched with PSI or PSII

et al. 2011

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The procedure of isolating the thylakoids and the thylakoid membrane fragments enriched with either photosystem I or photosystem II (PSI- and PSII-membranes) from Arabidopsis thaliana leaves was developed. It differed from the one used with pea and spinach in durations of detergent treatment and centrifugation, and in concentrations of detergent and Mg(2+) in the media. Both the thylakoid and the fragments preserved carbonic anhydrase (CA) activities. Using nondenaturing electrophoresis followed by detection of CA activity in the gel stained with bromo thymol blue, one low molecular mass carrier of CA activity was found in the PSI-membranes, and two carriers, a low molecular mass one and a high molecular mass one, were found in the PSII-membranes. The proteins in the PSII-membranes differed in their sensitivity to acetazolamide (AA), a specific CA inhibitor. AA at 5 × 10(-7) M inhibited the CA activity of the high molecular mass protein but stimulated the activity of the low molecular mass carrier in the PSII-membranes. At the same concentration, AA moderately inhibited, by 30%, the CA activity of PSI-membranes. CA activity of the PSII-membranes was almost completely suppressed by the lipophilic CA inhibitor, ethoxyzolamide at 10(-9) M, whereas CA activity of the PSI-membranes was inhibited by this inhibitor even at 5 × 10(-7) M just the same as for AA. The observed distribution of CA activity in the thylakoid membranes from A. thaliana was close to the one found in the membranes of pea, evidencing the general pattern of CA activity in the thylakoid membranes of C3-plants.

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“…Measurements have been carried out on whole bacterial cells, algae, chloro plasts, and fragments of chloroplasts and bacteria. Shear fields (65,192), electric fields (186), and magnetic fields (9 1, 194, 196--20 1) have all been used to orient samples, and a variety of polarized absorption (65,91,(181)(182)(183)(184)(185)(186)(187)(188)(189)(190)(191)(192)(193)(194)(195)(196), emission (21,195,196,199,200,202,203), and photoselection experiments (9 1, 165,169,170,196,200,[204][205][206][207][208][209][210] have been carried out. In spite of this diverse and extensive effort, only limited conclusions about molecular orientation can be drawn from the existing data.…”

Section: Heme Proteinsmentioning

confidence: 99%