Some virulence genes of Escherichia coli isolated from cloacal swabs of healthy Alagoas Curassows (Pauxi mitu) in Brazil

Abstract

tfraso@usp.br Birds of the Cracidae family (curassows, guans, and chachalacas) are endemic of the Neotropics and 50 species are currently classified. Brazil has 22 species, seven of which are considered threatened. The Alagoas Curassow (Pauxi mitu) species is considered extinct in the wild; but about 120 birds are alive in captivity. Conservation of this species depends entirely on correct management. Health reports of both wildlife and captive curassows are rare. In this study the presence of Escherichia coli was evaluated in 23 healthy Alagoas Curassows from two private breeding centres. E. coli was isolated from cloacal swabs, and the presence of genes encoding cytotoxic necrotising factor 1 (cnf1), alpha-haemolysin (hly), aerobactin (iuc), serum resistance (iss) and the following adhesions: S fimbriae (sfa), pili associated with pyelonephritis (pap) and temperature-sensitive haemagglutinin (tsh) were investigated. E. coli was isolated from 78.3% (18/23) of the birds, and the percentage of curassows colonized by E. coli was similar between the two facilities. From the 22 E. coli isolates, 15 (68.2%) were positive for at least one virulence factor by PCR, and the most frequently found gene was iss (50%). No curassows had clinical signs of disease. Nevertheless, the presence of some E. coli strains may be a concern to the wildlife in captivity. Additional health surveillance studies are essential to guarantee successful conservation programmes for threatened cracids in Brazil. correto. Informações sobre o status sanitário de mutuns são raras, tanto em vida livre quanto em cativeiro. Neste estudo a presença de Escherichia coli foi avaliada em 23 mutuns-do-nordeste sadios de dois criatórios particulares. E. coli foi isolada a partir de suabes cloacais, em seguida, foi avaliada a presença de genes que codificam fator citotóxi-co necrotizante 1 (cnf1), alfa-hemolisina (hly), produção de aerobactina (iuc) e resistência sérica (iss) e genes que codificam os seguintes fatores de virulência: fímbria S (sfa), pili associado à pielonefrite (pap) e hemaglutinina termosensível (tsh

Genotyping of virulent Escherichia coli obtained from poultry and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction

“…All the E. coli isolates were able to produce green metallic-sheen colonies on EMB agar, lactose-fermenting pink colonies on MacConkey agar, and amplified E16S gene giving 231 bp amplicon. The present results were in accordance with the findings of earlier studies from Srinagar, India [ 18 ] and Sao Paulo, Brazil [ 8 ], where E. coli was isolated from 80.2% and 78.3% of the poultry fecal samples tested.…”

“…Foodborne disease outbreaks, HUS, TTP, and HC are associated with certain STEC O-serogroups mainly including O157, O26, O91, O103, O121, O113, O111, O145, O45, and O128 as well as untypeable groups [ 3 - 5 ]. Compared to cattle and sheep, which act as major reservoirs of STEC, a limited number of reports have been published on STEC from poultry species and their genetic relatedness with human STEC [ 6 - 8 ].…”

Prevalence, Antimicrobial Resistance and Substantial Virulence-Associated Genes of Escherichia Coli Isolated From Colibacillosis in Neonatal Calves in Egypt

“…Interestingly, neither the tsh nor the iutA gene was detected in any tested samples in the current investigation, which attributed to variation in the results obtained by the researchers. In accordance with the previous study, (Saidenberg et al, 2013) illustrated that the frequency of the tsh gene in E. coli avian strains was 85.3%. Another investigation in Egypt by (Abdulgayeid et al, 2015) revealed that the prevalence of the tsh gene in E. coli of diarrheic calves was 100%.…”

Phenotypic and Genotypic Characterization of Gram negative bacteria Isolated from Birds of Prey (Raptors)

“…The result of examination of E.coli isolates for the presence of the increased serum survival gene (iss) as shown in photo (3) revealed that (3/11) 27.27% of the examined E.coli strains from raptors bearing the virulence gene (iss).this results is similar to that of Knobl et al (2011) who detected iss gene in 7/24 E. coli isolates in psittassine birds by ratio 29.2%. while higher percentage was detected by Saidenberg et al (2013) who found that the most frequent virulence factor was iss(11/22 isolates)by ratio 50% of E. coli isolates recovered from healthy Alagoas Curassows (Pauximitu) in Brazil The result of investigation of E.coli isolates for the presence of (stx1and stx2) as shown in photo(4) recorded that stx1 was not found in any examined E. coli strain (0/11) 0%, while stx2 was found in (4/11)36.36% of the examined E.coli strains. Similar results are obtained by (Lilian et al, 2017) who revealed that 3/401of E.colisamples are positive for stx2 gene (0.75%) distributed among the orders of Psittaciformes, Strigiformes and Columbiformes.…”