Natural
fibers with functionalities have attracted considerable
attention. However, developing facile and versatile strategies to
modify natural fibers is still a challenge. In this study, cotton
fibers, the most widely used natural fibers, were partially oxidized
by sodium periodate in aqueous solution, to give oxidized cotton fibers
containing multiple aldehyde groups on their surface. Then poly(hexamethylene
guanidine) was chemically grafted onto the oxidized cotton fibers
forming Schiff bases between the terminal amines of poly(hexamethylene
guanidine) and the aldehyde groups of oxidized cotton fibers. Finally,
carbon–nitrogen double bonds were reduced by sodium cyanoborohydride,
to bound poly(hexamethylene guanidine) covalently to the surface of
cotton fibers. These functionalized fibers show strong and persistent
antibacterial activity: complete inhibition against Escherichia coli and Staphylococcus
aureus was maintained even after 1000 consecutive
washing in distilled water. On the other hand, cotton fibers with
only physically adsorbed poly(hexamethylene guanidine) lost their
antibacterial activity entirely after a few washes. According to Cell
Counting Kit-8 assay and hemolytic analysis, toxicity did not significantly
increase after chemical modification. Attributing to the hydrophilicity
of poly(hexamethylene guanidine) coatings, the modified cotton fibers
were also more hygroscopic compared to untreated cotton fibers, which
can improve the comfort of the fabrics made of modified cotton fibers.
This study provides a facile and versatile strategy to prepare modified
polysaccharide natural fibers with durable antibacterial activity,
biosecurity, and comfortable touch.