Sorting CD4+ T Cells in Blood by Using Magnetic Nanoparticles Coated with Anti-CD4 Antibody

Khuat, N. T.; Nguyen, Vina; Phan, Tuan‐Nghia; Hoàng, Lục Huy; Thach, C. V.; Hai, Nguyen Hoang; Chau, Nguyet Trang Thanh

doi:10.3938/jkps.53.3832

Cited by 5 publications

(4 citation statements)

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“…MNP was dissolved in ethanol (1 mg mL −1 ) and 1% of 3‐aminopropyltriethoxysilane (Sigma) was treated 8 h for an amine‐modified MNP. Anti‐EpCAM and anti‐EGFR antibody (R&D systems) were immobilized using EDC method with amine‐modified MNP which previously reported method …”

Section: Methodsmentioning

confidence: 99%

Subtyping of Magnetically Isolated Breast Cancer Cells Using Magnetic Force Microscopy

et al. 2018

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Circulating tumor cell (CTC) which recently arisen as potential sources for monitoring and characterizing non-haematologic cancers and their metastatic derivatives. Immunomagnetic microbeads and magnetic nanoparticles (MNPs) have been extensively explored to isolate CTCs from blood samples. However, MNPs attached on the membrane protein are interrupted further analysis to distinguish the cancer subtype by consumption or blocking the target surface marker. Here, an MNP-mediated analysis method for surface marker expression profile by magnetic force microscopy (MFM) is described. Two MNPs, zinc ferrite and iron oxide, are showed distinct phase shift (-16.5° and -3.7°, respectively) signal on the MFM images. The antibody conjugated MNPs are successfully isolated target cells without giving damage to the cell. The MFM image of MNP decorated cells show clear differences between two breast cancer cell lines, MCF-7 and SK-BR-3, which proof the cancer subtyping property using MFM method. To confirmation of the surface marker consumption during the cell isolation, antibody-conjugated quantum dots and drug-loaded oleosome are treated on the cells, thereby MNP decorated cells are survived. This newly developed MFM analysis method provides a new direction to utilize the MNP for the surface marker expression phenotypes.

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Section: Methodsmentioning

confidence: 99%

Subtyping of Magnetically Isolated Breast Cancer Cells Using Magnetic Force Microscopy

et al. 2018

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“…Physicochemical properties of functional nanoparticles have recently drawn considerable attention due to their advantages in various applications [22,23]. For example, nanoparticles not only can be used for optical properties [24], electric properties [25], magnetic properties [23,[26][27][28], and catalytic properties [29] but also for biological properties [30,31], life science, environmental technology [32], diagnosis, and therapy [33][34][35][36].…”

Section: Laser Ablationmentioning

confidence: 99%

Synthesis and Characterization of ZnO-Nanostructured Particles Produced by Solar Ablation

Schiopu,

Oproescu,

Iana

et al. 2023

Materials

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Nowadays, nanotechnology offers opportunities to create new features and functions of emerging materials. Correlation studies of nanostructured materials’ development processes with morphology, structure, and properties represent one of the most important topics today due to potential applications in all fields: chemistry, mechanics, electronics, optics, medicine, food, or defense. Our research was motivated by the fact that in the nanometric domain, the crystalline structure and morphology are determined by the elaboration mechanism. The objective of this paper is to provide an introduction to the fundamentals of nanotechnology and nanopowder production using the sun’s energy. Solar energy, as part of renewable energy sources, is one of the sources that remain to be exploited in the future. The basic principle involved in the production of nanopowders consists of the use of a solar energy reactor concentrated on sintered targets made of commercial micropowders. As part of our study, for the first time, we report the solar ablation synthesis and characterization of Ni-doped ZnO performed in the CNRS-PROMES laboratory, UPR 8521, a member of the CNRS (French National Centre for Scientific Research). Also, we study the effect of the elaboration method on structural and morphological characteristics of pure and doped ZnO nanoparticles determined by XRD, SEM, and UV-Vis.

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“…To minimize this background interference, CD4 1 T cells can be magnetically sorted from other cells in the blood, followed by fluorescent signal detection. Hai et al (2008) and Khuat et al (2008) used Fe 3 O 4 MNPs coated with fluorescent-labeled antiCD4 antibody (antiCD4-MNPs) to count the CD4 1 T cells. The antiCD4-MNPs were prepared through covalent linking between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs.…”

Section: Cd4 1 Cell Separationmentioning

confidence: 99%

Nanoparticles: synthesis and applications

Nam

Luong

2019

Materials for Biomedical Engineering

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Sorting CD4+ T Cells in Blood by Using Magnetic Nanoparticles Coated with Anti-CD4 Antibody

Cited by 5 publications

References 1 publication

Subtyping of Magnetically Isolated Breast Cancer Cells Using Magnetic Force Microscopy

Subtyping of Magnetically Isolated Breast Cancer Cells Using Magnetic Force Microscopy

Synthesis and Characterization of ZnO-Nanostructured Particles Produced by Solar Ablation

Nanoparticles: synthesis and applications

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